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RT-PCR Primers for the Study of Apoptosis

Human ICE-b

600 bp

CPP32

540 bp

ICE-Lap-3

600 bp

Human Fas Antigen

653 bp

Rat Fas Antigen

661 bp

Mouse Fas Antigen

590 bp

Mouse Fas Ligand

551 bp

A variety of cell lines were used to generate cDNA libraries to test the specificity of the RT-PCR primers for studying apoptosis. The target genes and the expected size of the PCR product are listed in Table 1. Each 50-l PCR amplification contained 10- to 15-l aliquots of cDNA libraries, 5 l of 10X Taq polymerase buffer, 2 l of 100 M primer sets, 4 l of 2.5 mM dNTP mixture, 0.5 l of Taq2000 DNA polymerase (5 U/l), and 28.5 l of distilled, deionized H2
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