Stratagene has developed reverse transcriptase-mediated polymerase chain
reaction (RT-PCR) primer sets for genes whose products are important in the
induction or regulation of the apoptotic response. These primers will find
application in diverse disciplines, including developmental biology, immunology,
and tumor biology. By using these primers, researchers will be able to analyze
the transcription state of genes in uninduced cells and cells induced to undergo
The RT-PCR primer sets for studying the apoptotic response were designed to
meet several criteria: (1) The primers sets, based on known genomic sequences,
amplify a region that spans at least one intron. (2) To distinguish the
amplification products from genomic sequences, which are longer than the cDNA
products, the primer sets amplify PCR products that are 400 bp to 650 bp in
length. (3) The primers were synthesized as 18- to 27-mer oligonucleotides and
maintain close to 50% GC content whenever possible. (4) Each set of primers
amplifies only a specific target
Testing Primers with cDNA Templates
Target Genes and Expected Size of the PCR Product
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