Navigation Links
RT-PCR Primer Sets for Human and Mouse Mismatch Repair Genes

a probe in other analytical techniques, such as Northern blot hybridization, nuclease protection assays or in situ hybridization. In addition, these probes might be useful for screening cDNA libraries for gene homologues in related species. Because the mismatch repair genes serve an important function in maintaining the genetic integrity of the cell, they are likely to be expressed in detectable levels in most cell types. In this regard, they could be classified as housekeeping genes. Thus, the RT-PCR primer sets could be used as positive controls for cDNA templates in RT-PCR studies unrelated to mismatch repair.

PCR Amplifications Using Primer Sets

figure 1

The human RT-PCR primer sets for mismatch repair were used in amplification reactions containing either cDNA template from the human promyelocytic cell line (HL60) or human genomic DNA template (figure 1). Total RNA from the HL60 cell line was isolated using Stratagenes RNA Isolation Kit and quantitated by measuring absorbance at 260 nm. First-strand cDNA was synthesized in a 50-l volume using 5 g of total RNA and Stratagenes RT-PCR Kit according to the recommended protocol. Either 1 to 2 l of the cDNA or 25 to 50 ng of the human genomic DNA was used in 50-l PCR reactions using RoboCycler temperature cyclers. Each reaction was performed in 1X Taq polymerase buffer, 250 M of each dNTP, 10 pmol of each primer and 2.5 U of Taq2000TM DNA polymerase. The temperature cycling profile was as follows: One cycle at 95C for 3 minutes; 30 cycles of 95C for 30 seconds, TA (the recommended annealing temperature for each primer set) for 1 minute and 72C for 1 minute; one cycle at 72C for 10 minutes.
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. New Kit Generates Exceptionally Pure Total RNA for RT-PCR
2. Brilliant Core Reagent Kits for QPCR and QRT-PCR
3. Control RT-PCR Primers for Human Gene Transcripts with Varying Abundance
4. prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification
5. Performance Comparisons of Commercial RT-PCR Systems
6. RT-PCR Primers for the Study of Apoptosis
7. cMaster RTplusPCR System for increased sensitivity and product length in RT-PCR
8. Single-cell RT-PCR Analysis of Paramecium Primaurelia with the Eppendorf cMaster RTplusPCR System
9. Optimized Dengue RT-PCR
10. QuantiTect SYBR Green RT-PCR Kit
11. C. therm. Polymerase One-Step RT-PCR System
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: PCR Primer Sets for Human and Mouse Mismatch Repair Genes

(Date:1/22/2015)... Dr. Greg Leyer of UAS Labs recently was ... Pre-Conference seminar on probiotics in San Diego, CA. , ... conference for health care professionals. This year’s pre-conference seminar was ... in health. Dr. Leyer spoke about the emerging topics and ...
(Date:1/22/2015)... BRUNSWICK , Nueva Jersey , 22 de ... Award for Biomedical Research abre hoy su llamada a ... personas cuya investigación científica ha hecho, o tiene el ... la salud humana. Las nominaciones se aceptarán hasta el ...
(Date:1/22/2015)... 22, 2015  Derma Sciences, Inc. (Nasdaq: ... advanced wound care, announces that AMNIOEXCEL® and AMNIOMATRIX®, ... added to the Premier, Inc. Regenerative Skin Grafting ... the AMNIOEXCEL® and AMNIOMATRIX® product lines, which continue ...
(Date:12/24/2014)... 2014  Vermillion, Inc. (Nasdaq:   VRML), a bio-analytical ... that the Company closed its previously announced private ... Jack W. Schuler , Birchview Fund LLC and ... before offering expenses.  The proceeds will be used ...
Breaking Biology Technology:El Dr. Paul Janssen Award for Biomedical Research emite su llamada a los nominados para 2015 2El Dr. Paul Janssen Award for Biomedical Research emite su llamada a los nominados para 2015 3El Dr. Paul Janssen Award for Biomedical Research emite su llamada a los nominados para 2015 4Derma Sciences Expands Access of its AMNIOTIC TISSUE Product Line with New Premier, Inc. Agreement 2Derma Sciences Expands Access of its AMNIOTIC TISSUE Product Line with New Premier, Inc. Agreement 3Derma Sciences Expands Access of its AMNIOTIC TISSUE Product Line with New Premier, Inc. Agreement 4Derma Sciences Expands Access of its AMNIOTIC TISSUE Product Line with New Premier, Inc. Agreement 5Vermillion Announces Closing of Equity Financing 2Vermillion Announces Closing of Equity Financing 3
... that a new market research report is available in ... 2010 http://www.reportlinker.com/p0481350/China-Bio-breeding-Industry-Report-2010.html ... China reached RMB 29.8 billion, up 4.9% from a ... capable of R&D independently, but they seem inferior when ...
... University, the Salk Institute for Biological Studies, and other ... patient,s own brain cells, which then can be studied ... brings researchers a step closer to understanding the biological ... be used to study other mysterious diseases such as ...
... cooperative research center program (I/UCRC) will "provide a one-stop ... wide range of metamaterials." Dr. David Crouse, associate ... Engineering at The City College of New York, serves ... Participating institutions are The City University of ...
Cached Biology Technology:Reportlinker Adds China Bio-breeding Industry Report, 2010 2Reportlinker Adds China Bio-breeding Industry Report, 2010 3Reportlinker Adds China Bio-breeding Industry Report, 2010 4Reportlinker Adds China Bio-breeding Industry Report, 2010 5Scientists recreate brain cells from skin cells to study schizophrenia safely 2Scientists recreate brain cells from skin cells to study schizophrenia safely 3Grove School professor leads new metamaterials center 2Grove School professor leads new metamaterials center 3Grove School professor leads new metamaterials center 4
(Date:1/22/2015)... man-made form of insulin delivered by nasal spray may ... with mild cognitive impairment and Alzheimer,s disease dementia, according ... Forest Baptist Medical Center. The study,s subjects ... (MCI) or mild to moderate Alzheimer,s dementia (AD). Those ...
(Date:1/22/2015)... NEW YORK and POINT ROBERTS, Washington ... global news source covering leading sectors including technology and tech ... Personal Privacy marketplace featuring master pickpocket and security consultant ... wallet is stolen and talks about the Wocket™ biometric smart ...
(Date:1/22/2015)... , Europe,s most ... Patent Office to present a video retrospective of the most exciting ... Christofer Toumazou and Sophie Wilson   ... the Award   Starting on 22 January 2015 on   ...
Breaking Biology News(10 mins):Nasal Spray with Insulin Equivalent Shows Promise as Treatment for Adults with Mild Cognitive Impairment, Alzheimer's Dementia 2Nasal Spray with Insulin Equivalent Shows Promise as Treatment for Adults with Mild Cognitive Impairment, Alzheimer's Dementia 3Apollo Robbins Talks about the Illusion of Personal Safety and How Easily Your Wallet is Stolen; If a Wocket Wallet is Stolen the Thief Can't do Anything With it! 2Apollo Robbins Talks about the Illusion of Personal Safety and How Easily Your Wallet is Stolen; If a Wocket Wallet is Stolen the Thief Can't do Anything With it! 3Ten Years of the European Inventor Award: A Retrospective Look at the Inventors and Ideas That Have Changed Our Lives 2Ten Years of the European Inventor Award: A Retrospective Look at the Inventors and Ideas That Have Changed Our Lives 3
... 2009 A St. Louis-based team of plant and ... the developing world today announced the formation of ... involving St. Louis Children,s Hospital, The Donald Danforth ... Medicine. Though they share a commonality of ...
... 2009 - Temperature explains much of why cold-blooded ... longer at higher latitudes than at lower latitudes, according ... of the National Academy of Sciences ( PNAS ... candidate Santiago Salinas, both of Stony Brook University,s School ...
... STILLWATER, Okla. In a first to the ... Sciences and Natural Resources will receive $226,890 in grant ... Oklahoma Water Resources Research Institute. OWRRI and USGS ... encroachment and the water cycle in the tallgrass prairie, ...
Cached Biology News:Researchers team up to provide new hope for childhood hunger 2Researchers team up to provide new hope for childhood hunger 3Researchers team up to provide new hope for childhood hunger 4Warmer environment means shorter lives for cold-blooded animals 2Key OSU water research receives national funding 2
Mouse monoclonal antibody raised against a partial recombinant VEGFB. NCBI Entrez Gene ID = VEGFB...
... The Infinite 200 series of microplate ... or absorbance detection in an affordable, easy-to-use ... science applications. The unique modular design and ... researchers with a choice of a monochromator ...
...
The Discovery Series PDQuest 2-D analysis software is used for imaging, analyzing, and databasing two-dimensional electrophoretic gels. The version upgrade allows upgrade of an established version of...
Biology Products: