Navigation Links
RT-PCR Primer Sets for Human and Mouse Mismatch Repair Genes

nd hGTBP12 proteins. Some mouse analogs to previously characterized mismatch repair genes have also been identified. These genes include mMSH2,13 mPMS2 14 and GTMBP 15 (mGTBP). Rep-3 in mice is similar to the MutS gene but shares the greatest homology to yeast MSH3.16

Rapid advances in the field of DNA repair have begun to elucidate the precise role of human mismatch repair proteins.17 In the current model, hMSH2 and hGTBP form a dimer, which recognizes the mismatch and binds to it. Another complex formed by hPMS2 and hMLH1 subsequently recognizes and binds to the DNA-bound, hMSH2-hGTBP complex. Other proteins are then recruited to complete the processes of incision and repair. The human hPMS2 protein performs the analogous function of the PMS1 protein in yeast. The biochemical function of the hPMS1 protein is unclear; however, mutations in the hPMS1 gene, as well as hMSH2, hMLH1 and hPMS2 genes, have been associated with hereditary nonpolyposis colon cancer, a condition marked by microsatellite instability.

RT-PCR Primer Sets for Mismatch Repair

Stratagenes RT-PCR primer sets for mismatch repair are designed to amplify cDNA segments representing transcripts of currently characterized mismatch repair genes in humans and mice. Because the RT-PCR primer sets amplify regions that span intron-exon boundaries, amplification using genomic DNA templates produces either no product at all or one that can easily be distinguished from the RT-PCR product by gel electrophoresis. The primer sets can be used in conjunction with quantitative RT-PCR techniques to study gene expression in areas such as genetic toxicology, where changes in the regulation of these genes may be indicative of the genotoxicity of a substance or its biologically activated form. The primer sets may also be used to quickly amplify a region of the featured genes for later use as
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. New Kit Generates Exceptionally Pure Total RNA for RT-PCR
2. Brilliant Core Reagent Kits for QPCR and QRT-PCR
3. Control RT-PCR Primers for Human Gene Transcripts with Varying Abundance
4. prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification
5. Performance Comparisons of Commercial RT-PCR Systems
6. RT-PCR Primers for the Study of Apoptosis
7. cMaster RTplusPCR System for increased sensitivity and product length in RT-PCR
8. Single-cell RT-PCR Analysis of Paramecium Primaurelia with the Eppendorf cMaster RTplusPCR System
9. Optimized Dengue RT-PCR
10. QuantiTect SYBR Green RT-PCR Kit
11. C. therm. Polymerase One-Step RT-PCR System
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: PCR Primer Sets for Human and Mouse Mismatch Repair Genes

(Date:1/22/2015)... 2015 The laboratory information management systems ... a number of technological advancements due to factors such ... need to integrate healthcare systems, and increasing government support ... system integration. Key players in the market focus on ...
(Date:1/22/2015)... San Diego, California (PRWEB) January 22, 2015 ... R Promotional Offer to their comprehensive portfolio of ... Offer allows customers to purchase a Centrifuge 5424/5424 R ... Pipette Bundle of either Eppendorf Research plus or Eppendorf ...
(Date:1/22/2015)... (PRWEB) January 22, 2015 Controlled Substance ... Alliance, which helps companies check the legal requirements around ... expand to China. , As their international operations ... globally adopting software solutions built as a result of ...
(Date:1/22/2015)... 22 de enero de 2015  El Dr. Paul ... llamada a nominaciones para 2015. Este prestigioso premio reconoce ... tiene el potencial para hacer, contribuciones destacadas para la ... hasta el 15 de marzo de 2015 a través ...
Breaking Biology Technology:Laboratory Information Management Systems Market is Expected to Reach $1,323.6 Million by 2019 - New Research Report by MarketsandMarkets 2Laboratory Information Management Systems Market is Expected to Reach $1,323.6 Million by 2019 - New Research Report by MarketsandMarkets 3Laboratory Information Management Systems Market is Expected to Reach $1,323.6 Million by 2019 - New Research Report by MarketsandMarkets 4Eppendorf Announces Promotional Bundle Which Includes: Eppendorf Centrifuge 5424, 3-Pack of Pipettes, and Tips - Available Now at Pipette.com 2Global Compliance Service for Controlled Substances to Expand to China 2El Dr. Paul Janssen Award for Biomedical Research emite su llamada a los nominados para 2015 2El Dr. Paul Janssen Award for Biomedical Research emite su llamada a los nominados para 2015 3El Dr. Paul Janssen Award for Biomedical Research emite su llamada a los nominados para 2015 4
... information security firm Symantec released the 11th edition ... Symantec's attempt to get out of the day-to-day and see ... ominous, according to Dave Cole, a director in the Symantec ... game today is data theft, and the creation of malicious ...
... the growing dependence on transportation infrastructure between Illinois ... connect new and existing developments was highlighted. Building ... having a great supporting infrastructure, but I was ... reader Larry Zielonka, a CPA, commented that the ...
... reported net income of $113.5 million during the first ... revenues rising 11 percent over the same quarter in 2006. ... services industry, said net income was down from the $116.2 ... per share were slightly higher than the $0.64 in the ...
Cached Biology Technology:Symantec: Cyber fraudsters look for low-hanging fruit 2Symantec: Cyber fraudsters look for low-hanging fruit 3Symantec: Cyber fraudsters look for low-hanging fruit 4Symantec: Cyber fraudsters look for low-hanging fruit 5Symantec: Cyber fraudsters look for low-hanging fruit 6Symantec: Cyber fraudsters look for low-hanging fruit 7Mega-Metro Center may go beyond Chicago and Wisconsin 2Mega-Metro Center may go beyond Chicago and Wisconsin 3Mega-Metro Center may go beyond Chicago and Wisconsin 4Mega-Metro Center may go beyond Chicago and Wisconsin 5
(Date:1/22/2015)... Issued by Small Cap IR. In 2011, Nilson Report,s annual ... merchants on the leading payment cards rose to $135.3 billion ... prepaid cards reached 6.54 billion.  Eight million credit card users ... 2012, and credit and debit card fraud resulted in $11.27 ...
(Date:1/22/2015)... has launched its new NXG series of time clocks with ... higher standard for collecting attendance and labor data and complements ... touch screen interface and seamless connection to cloud-based iSolved, the ... for the small to mid-size employer. The G2 ...
(Date:12/22/2014)... 22, 2014  The 2014 Holiday Season may be the ... Intelligence reports that the long anticipated floodgates for consumer ... demand for smart phones, tablets, and wearable mobile devices ... 2.5 billion users with nearly 4.8 billion biometric devices ...
Breaking Biology News(10 mins):$4.80 Near Term Price Target with "Buy" given by CapRock Research for NXT-ID, Inc. (NASDAQ: NXTD) issued by Small Cap IR 2Infinisource's NXG series sets new time clock standard 2First Season of Holiday Shopping with Mobile Biometric Payments Wraps Up With a Present for Biometrics Industry: A Rosy Forecast for More Than 2 Billion Users of 4.8 Billion Mobile Biometric Devices by 2020 2
... discovered how a network of repair proteins enables bacteria to ... the DNA molecules that carry the instructions necessary for living ... at the University of Bristol and published in Molecular ... between the DNA repair systems of bacteria and humans than ...
... 38 million people worldwide are currently infected with HIV ... For scientists to design treatment therapies that are effective ... about how the virus mutates and develops resistance to ... biomedical engineer Stephanie Willerth has significantly advanced the understanding ...
... Researchers presented Phase III efficacy data from the GeparQuinto ... in combination with chemotherapy for patients with early breast ... Cancer Symposium, held Dec. 8-12. "We had ... combination of chemotherapy, trastuzumab, with the new combination of ...
Cached Biology News:New research reveals details of microbe's extraordinary maintenance and repair system 2UVic biomedical engineer 'outsmarts' HIV 2Phase III study compared neoadjuvant therapy with lapatinib or trastuzumab for early breast cancer 2