Navigation Links
RT-PCR Primer Sets for Human and Mouse Mismatch Repair Genes

Primer sets facilitate study of DNA mismatch repair

Mark Dycaico
Stratagene Cloning Systems

Stratagene has designed RT-PCR primer sets from the cDNA sequences of mismatch repair genes. These genes code for specialized proteins that participate in correcting areas of base mispairing in DNA. We have assembled primer pairs specific for human (hMSH2, hMLH1, hGTBP, hPMS1 and hPMS2) and mouse (mMSH2, mGTBP, mPMS2 and Rep-3) mismatch repair genes. The primer pairs can be used to detect mismatch repair gene transcripts by the reverse transcriptase polymerase chain reaction (RT-PCR). The RT-PCR primer sets for mismatch repair may also be used as positive controls for RT-PCR experiments unrelated to mismatch repair.

Mismatched base pairs in DNA can form in vivo through processes such as replication errors, heteroduplex formation, deamination of 5-methylcytosine and the presence of DNA adducts.1 Cells cope with mismatches by enlisting specialized proteins that can recognize, excise and correct mismatched bases. This process is known as DNA mismatch repair. In addition to correcting single base mispairing, mismatch repair pathways have been suspected to play a role in controlling microsatellite instability in proto-oncogenes2 and in modulating adaptive mutation in nondividing cells.3

The characterization of the E. coli MutHLS mismatch repair system4 led to the identification of an analogous eukaryotic system in Saccharomyces cerevisiae. Proteins in this system include a yeast homologue of MutS, called MSH2,5 and two yeast homologues of MutL, called MLH16 and PMS1.7 Discovery of the human mismatch repair homologues soon followed with the identification of the genes for the hMSH2,8 hMLH1,9 hPMS1,10 hPMS211 a
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. New Kit Generates Exceptionally Pure Total RNA for RT-PCR
2. Brilliant Core Reagent Kits for QPCR and QRT-PCR
3. Control RT-PCR Primers for Human Gene Transcripts with Varying Abundance
4. prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification
5. Performance Comparisons of Commercial RT-PCR Systems
6. RT-PCR Primers for the Study of Apoptosis
7. cMaster RTplusPCR System for increased sensitivity and product length in RT-PCR
8. Single-cell RT-PCR Analysis of Paramecium Primaurelia with the Eppendorf cMaster RTplusPCR System
9. Optimized Dengue RT-PCR
10. QuantiTect SYBR Green RT-PCR Kit
11. C. therm. Polymerase One-Step RT-PCR System
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: PCR Primer Sets for Human and Mouse Mismatch Repair Genes

(Date:7/23/2008)... SAN DIEGO, July 23 /- An...ced today that it will report second quarter 2008,...er the U.S. financial,markets close., Anadys will... second,quarter 2008 financial results and highlig...ograms on Wednesday, July 30, 2008 at 5:00 p.m. Ea...
(Date:7/23/2008)... CHAPEL HILL, N.C., July 23 /- Best...ishing firm that is a world-leader in the,field of...eutical, biotech,medical device and health care i...ess function specific Webinars with a focus on,pha...s Scheduled in July 2008, Best Practices, LLC,s W...
(Date:7/23/2008)... DETROIT, July 23 /- Cara... announced the resignation as a Director of,the Co...a Director of the,Company of Gurpartap ("GP") Sing...trategies of the Company, effective July 22, 2008....nt of Business,Strategies in July 2007, Mr. Singh ...
(Date:7/23/2008)... NORTH VANCOUVER, BC, July 23 /PRNewswire-FirstC...r the "Company") announced today that the,securiti...n the Provinces,of British Columbia, Alberta, Mani...ade orders against the Company effective July 22, ...ade orders outstanding against the,Company., The ...
Breaking Biology Technology:Anadys Pharmaceuticals to Report Second Quarter 2008 Financial Results 2Web-Conferences by Best Practices, LLC for July 2008 2Caraco Pharmaceutical Laboratories, Ltd. Announces Resignation from Board of Directors of Jitendra Doshi and Appointment of GP Singh Sachdeva 2Chromos Announces Full Revocation of Cease Trade Orders 2
... Laboratories, which are found in all engineeri...the education experience. Not only do laboratorie... also bring the course theory alive so students c... affect real-world measurements and control algor... expense and its maintenance can be difficult. Te...
... by Erwin Hauser, Chief Engineer, KNF Neuberger,... Where gases with high moisture content must be ev...te may collect in the vacuum pump head. Consequent...e more time to evacuate the designated volume. New...head during the evacuation process and thus drama...
...-Jin Yoon and Kyung-Ah Lee Infertility Medical Cen... , Abstract , Laser Capture Microdisse...ts the rapid and reliable procurement of pure cell...fter LCM, RNA isolated from pure cell populations ...sense RNA (aRNA) for hybridization, and the differ...
..., , Modern technologies developed in recent...o support biomedical research. The ability to stud... genomic material to detect genetic disordersmade ...mePlex whole-genome amplification (WGA) systemis...f high-throughput genomics and DNA sequencing has ...
Other Biology Technology:Distance-Learning Remote Laboratories using LabVIEW 2Distance-Learning Remote Laboratories using LabVIEW 3Distance-Learning Remote Laboratories using LabVIEW 4Distance-Learning Remote Laboratories using LabVIEW 5Distance-Learning Remote Laboratories using LabVIEW 6Distance-Learning Remote Laboratories using LabVIEW 7Distance-Learning Remote Laboratories using LabVIEW 8Distance-Learning Remote Laboratories using LabVIEW 9Distance-Learning Remote Laboratories using LabVIEW 10Distance-Learning Remote Laboratories using LabVIEW 11Distance-Learning Remote Laboratories using LabVIEW 12Self-Drying Vacuum Pumps... the solution for applications producing condensation 2Self-Drying Vacuum Pumps... the solution for applications producing condensation 3Self-Drying Vacuum Pumps... the solution for applications producing condensation 4Differential Gene Expression Analysis of Pure Cell Populations from Frozen UterineTissue Using Laser Capture Microdissection (LCM) and cDNA Microarrays 2Differential Gene Expression Analysis of Pure Cell Populations from Frozen UterineTissue Using Laser Capture Microdissection (LCM) and cDNA Microarrays 3Differential Gene Expression Analysis of Pure Cell Populations from Frozen UterineTissue Using Laser Capture Microdissection (LCM) and cDNA Microarrays 4Differential Gene Expression Analysis of Pure Cell Populations from Frozen UterineTissue Using Laser Capture Microdissection (LCM) and cDNA Microarrays 5Differential Gene Expression Analysis of Pure Cell Populations from Frozen UterineTissue Using Laser Capture Microdissection (LCM) and cDNA Microarrays 6Differential Gene Expression Analysis of Pure Cell Populations from Frozen UterineTissue Using Laser Capture Microdissection (LCM) and cDNA Microarrays 7Differential Gene Expression Analysis of Pure Cell Populations from Frozen UterineTissue Using Laser Capture Microdissection (LCM) and cDNA Microarrays 8Amplification of genome-representative DNA from limited sources with GenomePlex WGA technology for use in genetic alterations studies 2Amplification of genome-representative DNA from limited sources with GenomePlex WGA technology for use in genetic alterations studies 3
(Date:7/22/2008)...ring professor at The University of Texas at Austi...tolerant membrane that should simplify the water d...ter and possibly reducing greenhouse gases. , "I...with better membranes, it will be less expensive t...the availability of clean water around the world,"...
(Date:7/22/2008)... Spanish . , ,, Plants undergo stress bec... or to excess of light. A research team from the U...substances that are triggered in plants to protect... that is best suited to the environment during ref... , Droughts, extreme temperatures, contamination...
(Date:7/22/2008)...hat a frog that lives near noisy springs in centra...encies, much like the tuner on a radio can shift f... example of an animal that can actively select wha...he findings, from a collaborative effort led by th...ifornia at Los Angeles, appear this week in Proce...
(Date:7/21/2008)... now if it wants to reach its Minnesota Next Gener...emissions by 15 percent by 2015, according to a te...ublic policy researchers. , The research team, w...modeled emissions for Minnesota and found that it ...025 and 80 percent by 2050 and possibly exceed tho...
Breaking Biology News(10 mins):New chlorine-tolerant, desalination membrane hopes to boost access to clean water 2Measuring the stress of forested areas 2Measuring the stress of forested areas 3Ultrasonic frogs can tune their ears to different frequencies 2Ultrasonic frogs can tune their ears to different frequencies 3Action needed now for Minnesota to reach goals in reducing greenhouse gas emissions by 2015 2Action needed now for Minnesota to reach goals in reducing greenhouse gas emissions by 2015 3Stanford biologist awarded 2008 Sophie Prize in Norway 13600 1The Pennsylvania Breast Cancer Coalition Announces Last Chance to Enter Makeover for Mom Contest 13598 1The Pennsylvania Breast Cancer Coalition Announces Last Chance to Enter Makeover for Mom Contest 13598 2Is Your Dog a Canine stein or a Bonehead 3F 13596 1Is Your Dog a Canine stein or a Bonehead 3F 13596 2Is Your Dog a Canine stein or a Bonehead 3F 13596 3Genetic Test Predicts Response to Warfarin 13594 1Genetic Test Predicts Response to Warfarin 13594 2Genetic Test Predicts Response to Warfarin 13594 3
...tain different gene mutations, but just how these ... been a mystery. , A new clue comes from scientist...ll Schools of Medicine and Public Health. Their re...m birth to 20 years old, may specifically increase... different mutation, on the NRAS gene, was found i...
...e shown a significant increase in Northern Ireland...ct men, older people and those living in more affl... the June issue of British Journal of Dermatology....stics for nearly 23,000 patients over a 12-year pe...and a 62 per cent increase in skin cancer samples ...
... team has published a new mechanistic description ... in the early stages of tumor metastasis. This new...eloping anti-metastasis therapies. , Scientists k...em, but they don’t understand the details of how t... issue of the journal Cancer Cell, EPFL Professor ...
...k physicist has uncovered how female cells are abl...es and why that choice is always lucky. , Human ma...ave two X chromosomes. This means that in an early...egg the cells need to silence one of those two X c...d problems with the process are related to serious...
Other Biology News:Sun exposure early in life linked to specific skin cancer gene mutation 2Rising skin cancer rates are more likely to affect wealthy people, says 12-year review 2Rising skin cancer rates are more likely to affect wealthy people, says 12-year review 3Migration alert -- How tumor cells home in on the lymphatic system 2Physicist cracks women's random but always lucky choice of X chromosome 2
...ally and functionally related BMPs, which are memb...lly identified as protein regulators of cartilage ...yogenesis and morphogenesis of various tissues and..., chemotaxis and apoptosis of various cell types, ...
RABBIT ANTI HUMAN TRRAP (CT) Immunogen: Recombinant C-terminal kinase-related domain of human TRRAP.
RABBIT ANTI HUMAN CHYMOTRYPSIN
MSE ANTI CAN. CD3:FITC/RAT ANTI CD4:RPE
Biology Products: