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RNeasy Mini, Midi, and Maxi Kits

l and northern blot of total RNA purified with the RNeasy Maxi Kit. 10 g of total RNA isolated from each source was loaded per lane. All tissues were from mouse. Yeast: Saccharomyces cerevisiae; E. coli: HB101. 32P-labeled probes recognized (G) GAPDH; (E) translation elongation factor EF-1a ; and (O) outer membrane protein A sequences. (E and O were kindly provided by P. Philippsen, University of Basel, Switzerland and U. Henning, Max Planck Institute of Biology, Tbingen, Germany, respectively.) B. subtilis was not probed. M: 0.249.5 kb RNA ladder. 7.5 kb band (indicated) in embryo, Huh7, and HeLa cell lanes is a nuclear precursor RNA.
RT-PCR of RNA from >100 Cells RT-PCR of total RNA isolated with the RNeasy Mini Kit from the indicated numbers of HeLa cells. 10 l (1/5) of eluate was digested with RNase-free DNase and reverse transcribed with oligo-dT primer. 2.5 l (1/20) of the cDNA mix was used in 50 l PCR. A 452 bp fragment of GAPDH was amplified. C: negative control; C+: positive control; M: 100 bp ladder.
Total RNA Yields Obtained with RNeasy Mini, Midi, and Maxi Kits Amounts can vary due to developmental stage, species, growth conditions used, etc. Since the RNeasy procedure enriches for RNA species >200 nt, RNA yield does not include 5S rRNA, tRNAs, or other low-molecular-weight RNAs.
* Using the specialized protocol for heart, muscle, and skin tissue, included in handbook. Proteinase K (required for this protocol) is not provided.
Higher yields can be obtained from stabilized bacteria using RNeasy Protect Bacteria Kits.<
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