RNeasy Mini, Midi, and Maxi Kits allow efficient purification
of total RNA from very small (see figure "RT-PCR of
RNA from =100 Cells
") to very large amounts of starting material.
Total RNA is easily purified from animal cells or tissues, Gram-positive
or Gram-negative bacteria,* or yeast (See table "Total
RNA Yields Obtained with RNeasy Mini, Midi, and Maxi Kits
figure "High-Quality RNA from a Variety of Samples
Protocols are also included for isolation of cytoplasmic RNA from
eukaryotic cells as well as for cleanup of partially purified RNA, in vitro
transcripts, and RNA from enzymatic reactions. A variety of special application
protocols, including a protocol for vacuum processing of RNeasy mini columns,
are also available. QIAshredder Homogenizers for convenient cell-lysate
homogenization are available as an accessory.
RNeasy Kits provide the highest-quality RNA with minimum copurification
of DNA. For certain RNA applications that are sensitive to very small
amounts of DNA, the residual amounts of DNA remaining can be removed using
the RNase-Free DNase Set for convenient on-column DNase treatment during
the RNeasy procedure.
* Lysozyme (required for bacterial samples) is not provided.
High-Quality RNA from a Variety of Samples
Lyticase, zymolase, or glass beads (required for yeast samples)
are not provided.
Additional buffer required. The buffer composition is provided
in the protocols.
Formaldehyde agarose ge
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. RNeasy Protect Mini, Midi, and Maxi Kits2
. miRNeasy Mini Kit and miRNeasy 96 Kit