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RNeasy Mini, Midi, and Maxi Kits

l 8002400 l RNeasy Mini, Midi, and Maxi Kits allow efficient purification of total RNA from very small (see figure "RT-PCR of RNA from =100 Cells") to very large amounts of starting material. Total RNA is easily purified from animal cells or tissues, Gram-positive or Gram-negative bacteria,* or yeast (See table "Total RNA Yields Obtained with RNeasy Mini, Midi, and Maxi Kits" and figure "High-Quality RNA from a Variety of Samples"). Protocols are also included for isolation of cytoplasmic RNA from eukaryotic cells as well as for cleanup of partially purified RNA, in vitro transcripts, and RNA from enzymatic reactions. A variety of special application protocols, including a protocol for vacuum processing of RNeasy mini columns, are also available. QIAshredder Homogenizers for convenient cell-lysate homogenization are available as an accessory.

RNeasy Kits provide the highest-quality RNA with minimum copurification of DNA. For certain RNA applications that are sensitive to very small amounts of DNA, the residual amounts of DNA remaining can be removed using the RNase-Free DNase Set for convenient on-column DNase treatment during the RNeasy procedure.

* Lysozyme (required for bacterial samples) is not provided.
Lyticase, zymolase, or glass beads (required for yeast samples) are not provided.
Additional buffer required. The buffer composition is provided in the protocols.

High-Quality RNA from a Variety of Samples Formaldehyde agarose ge
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