Navigation Links
RNase Protection Assay (RPA),,,Using DIG-Labeled RNA Probes

he sample by the appearance of an appropriately sized probe fragment.

Factors Influencing the Assay

Transcript quality
  • Long transcripts tend to give incomplete transcribed probes. We suggest the use of a relatively small probe for this assay (100300 nucleotides).
  • RNase contamination
  • Presence of transcription-termination signals in the template.
  • Wraparound transcripts resulting from the use of REs that produce either a 3 overhang or a blunt end. It is strongly recommended to use a RE that produces a 5 overhang.

Quality of target RNA
  • RNase contamination: When probe fragmentation is observed in the protection assay, check the integrity of your RNA sample by agarose electrophoresis.
  • DNA contamination

Amount of sample and labeled probe

The sample size is usually in the milligram-range and the amount of labeled probe is around 500 pg1,000 pg.





'"/>

Source:


Page: All 1 2

Related biology technology :

1. Protector RNase Inhibitor
2. Protector RNase Inhibitor Enhance the Protection of RNA against Degradation
3. Inducing RNAi with siRNA Cocktails Generated by RNase III
4. Dicer vs. RNase III for Preparation of siRNA Cocktails
5. Combat RNase Contamination in the Lab
6. Measuring RNase Activity A Real-time Kinetic Analysis
7. RNase Activity in Mouse Tissue: Classification, Hierarchy, and Methods for Control
8. Nuclease Protection Assays: The Basics
9. A Positive Selection Assay for Mutation Analysis in Big Blue Animals
10. Luminol-enhanced Assay for Superoxide Anion (O2 face="Symbol" )
11. Organize Your Data from the Big Blue Mutation Assay

Post Your Comments:
*Name:
*Comment:
*Email:
TAG: RNase Protection Assay RPA Using DIG Labeled RNA Probes

(Date:11/6/2009)... The agenda for Arrowhead&rsqu...ence, which is set to take place on February 22-24...s three day conference we will highlight key issue...kers to share their experiences. , ... -- The agenda for Arrowhead’s 2010 Persona...
(Date:11/5/2009)... GAITHERSBURG, Md., Nov. 5 /PRNewswire-FirstCall/ ...ts financial results for the third quarter ended S...3.6 million ($0.04 per share) for the three months...f $6.8 million ($0.08 per share) in the comparable...ber 30, 2009, GenVec,s net loss was $14.1 million ...
(Date:11/5/2009)... PARSIPPANY, N.J., Nov. 5 DSM Phar...de a manufacturing alliance with Galenix, Saint Je...d commercial scale manufacturing partner for Galen...M and Galenix will collaborate on business develop...ery technologies, based on the strength of Galenix...
(Date:11/5/2009)... RANCHO CORDOVA, Calif., Nov. 5 /PRNewswire-FirstC...ading supplier of innovative products for processi...sults for the first quarter of fiscal 2010. ,, ...any reported revenues of $5.2 million, a 15 percen...rst quarter a year ago. The Company said the key c...
Breaking Biology Technology:Leaders in Biopharma and Molecular Diagnostics to Discuss Key Issues and Opportunities in Forming Partnerships at 2010 Personalized Medicine Partnerships Conference 2Leaders in Biopharma and Molecular Diagnostics to Discuss Key Issues and Opportunities in Forming Partnerships at 2010 Personalized Medicine Partnerships Conference 3GenVec Reports Third Quarter 2009 Financial Results 2GenVec Reports Third Quarter 2009 Financial Results 3GenVec Reports Third Quarter 2009 Financial Results 4GenVec Reports Third Quarter 2009 Financial Results 5GenVec Reports Third Quarter 2009 Financial Results 6DSM Pharmaceutical Products Forms Manufacturing Alliance With Galenix 2DSM Pharmaceutical Products Forms Manufacturing Alliance With Galenix 3ThermoGenesis Reports 15 Percent Increase in First Quarter Revenues; Improved Bottom Line 2ThermoGenesis Reports 15 Percent Increase in First Quarter Revenues; Improved Bottom Line 3ThermoGenesis Reports 15 Percent Increase in First Quarter Revenues; Improved Bottom Line 4ThermoGenesis Reports 15 Percent Increase in First Quarter Revenues; Improved Bottom Line 5ThermoGenesis Reports 15 Percent Increase in First Quarter Revenues; Improved Bottom Line 6ThermoGenesis Reports 15 Percent Increase in First Quarter Revenues; Improved Bottom Line 7ThermoGenesis Reports 15 Percent Increase in First Quarter Revenues; Improved Bottom Line 8
.../PRNewswire-FirstCall/ - OncoGenex Pharmaceuticals...ry results of a Phase 1 trial presented during an ...cal Oncology (ASCO) Annual Meeting. Preliminary re...l tolerated as a monotherapy. In addition, OGX-427...at all doses evaluated as well as evidence of redu...
...es Therapeutics, Inc. (Nasdaq: TPTX ) today anno...after consideration of potential strategic and fin...sts of the Company and its stockholders to liquida...y. The Company,s Board of Directors has unanimousl...f the Company (the "Plan of Dissolution") subject ...
...bbott as One of the Best Places to Work in Industr...tly named one of the Top 10 companies on The Scie..." list. Abbott was ranked No. 6 on the list of La...s the sixth time that Abbott has been on the list ...ies in 2003. This prestigious list is based on a ...
Other Biology Technology:OncoGenex Pharmaceuticals Announces OGX-427 Treatment Demonstrates Safety, Evidence of Declines in Circulating Tumor Cells and Reductions in Tumor Markers in a Phase 1 Cancer Trial 2OncoGenex Pharmaceuticals Announces OGX-427 Treatment Demonstrates Safety, Evidence of Declines in Circulating Tumor Cells and Reductions in Tumor Markers in a Phase 1 Cancer Trial 3OncoGenex Pharmaceuticals Announces OGX-427 Treatment Demonstrates Safety, Evidence of Declines in Circulating Tumor Cells and Reductions in Tumor Markers in a Phase 1 Cancer Trial 4OncoGenex Pharmaceuticals Announces OGX-427 Treatment Demonstrates Safety, Evidence of Declines in Circulating Tumor Cells and Reductions in Tumor Markers in a Phase 1 Cancer Trial 5TorreyPines Therapeutics Announces its Board of Directors' Approval of Plan of Liquidation and Dissolution 2TorreyPines Therapeutics Announces its Board of Directors' Approval of Plan of Liquidation and Dissolution 3Abbott Named One of the Top 10 Companies for Scientists 2Abbott Named One of the Top 10 Companies for Scientists 3
(Date:11/4/2009)...at,s the cost of saving a teenage life? When it co...prescription medication abuse, there,s no price ta...naware that the lock-boxes they,ve installed are n...b of prescription drugs. That,s why MEDNET(TM), a ...s, has formed an exclusive partnership with Axius ...
(Date:11/4/2009)...l Rainmakers, Inc., an intellectual property holdi...announced that the company has exited its "stealth...ts suite of technologies designed to leverage cutt...g the past four years, the company has spent tens ...ies of core--but expandable--technologies built ar...
(Date:11/4/2009)... Software, Inc., the commercial arm of the Open Mi...enmicroscopy.org ), is proud to announce its coll...y,s exciting new project titled "The Cell: An Imag...ulus grant through the National Institutes of Heal... the American Reinvestment and Recovery Act, to bu...
Breaking Biology News(10 mins):MEDNET Launches MedSafe to Prevent Teenage Prescription Drug Abuse 2MEDNET Launches MedSafe to Prevent Teenage Prescription Drug Abuse 3Global Rainmakers Brings Cutting-Edge Biometric Security Products to Reality 2Global Rainmakers Brings Cutting-Edge Biometric Security Products to Reality 3Glencoe Software to Support the ASCB 'Stimulus Grant' to Build Virtual Image Library of the Cell 2Palkion Selects Novel Oral Development Candidate for the Treatment of Anemia 12813 1Palkion Selects Novel Oral Development Candidate for the Treatment of Anemia 12813 2Palkion Selects Novel Oral Development Candidate for the Treatment of Anemia 12813 3Jillian Michaels Americas Toughest Trainer Announces New Line of Weight Loss Supplements 50243 1Jillian Michaels Americas Toughest Trainer Announces New Line of Weight Loss Supplements 50243 2Jillian Michaels Americas Toughest Trainer Announces New Line of Weight Loss Supplements 50243 3New Source of Information on Psoriasis Medications 50240 1New Source of Information on Psoriasis Medications 50240 2
...d that modern lifestyle habits may play a bigger r... , A review of the scientific evidence over the ...toothpaste, good oral hygiene and health education...decay. The research is published online in a Suppl...or Monty Duggal, an author of the review explained...
...roscientists at Georgetown University Medical Cent...vity looks like when someone anticipates an action...bruary 25 issue of the Journal of Neuroscience , ...activity in areas of the brain responsible for pre...y using functional magnetic resonance imaging (fMR...
...26, 2009) The Wildlife Conservation Society (WCS),...nt Facility (GEF), announced today a commitment of... range. WCS will lead a new project, Tiger Future...ations with long-term field experience in tiger c...cat,s geographical range in Asia., The Tiger Futu...
Other Biology News:Researchers capture wave of brain activity linked to anticipation 2Tigers get a stimulus plan 2Tigers get a stimulus plan 3
Mouse Anti-Vesicular Glutamate Transporter 2 (VGLUT2) Antibody, Unconjugated from CHEMICON
Proteinase K Buffer from BD Biosciences Pharmingen
RNase Buffer from BD Biosciences Pharmingen
Mouse cAMP / Ca2+ Signaling PathwayFinder, PCR Array for expression profiling from SuperArray Bioscience
Biology Products: