Navigation Links
RNase Activity in Mouse Tissue: Classification, Hierarchy, and Methods for Control

Protecting RNA During Isolation
RNase activity varies dramatically across different tissues. A comparison of total RNase activities for 8 different mouse tissues showed that total RNase activity spans a 181,000-fold range from pancreas to brain (Figure 1), which highlights the level of RNase control required for successful RNA isolation.

Figure 1. Quantitative Hierarchy of RNase Activity in Mouse Tissues. Mouse tissues were dounced in non-denaturing, neutral pH buffer (50 mM NaCl and 1 mM Mg2+), and total RNase activity was measured using Ambions fluorescence-based RNaseAlert QC System. Lysates were treated with EDTA (5 mM) or Ambions RNase Inhibitor protein (40 U/100 l reaction).

The tissues profiled for intrinsic RNase activity belonged in one of two groups. While RNases in Group A tissues were insensitiveto EDTA (and thus, Mg2+ independent), these tissues contained very high levels of RNase activity that were effectively inhibited by Ambions recombinant RNase Inhibitor, an inhibitor of RNase A-type enzymes. Group B tissues exhibited much lower levels of RNase activity that were more thoroughly inhibited by EDTA than by Ambions RNase Inhibitor (both before and after heating the lysates to inactivate endogenous inhibitors). Because RNase A enzyme activity is unaffected by the addition of EDTA, whereas other RNases are usually EDTA-sensitive, these results suggest that the RNas e A family of enzymes are the source of RNase activity in Group A tissues, whereas a more functionally heterogeneous group of RNases are active in Group B tissues.

For RNA isolation from tissues containing very high levels of RNase, we recommend TRI Reagent (see RNA, DNA, and Protein from a Single Sample and High Throughput RNA Isolation for High Performance Microarray Analysis). For tissues exhibiting more typical levels of RNase activity, Ambions convenient hands-free MELT Total RNA Isolation System maximizes yields with minimal handling (see Watch Your Tissue MELT into High Quality Total RNA).


Scientific Contributors

Julie Krosting, Gary Latham Ambion, Inc.


back to top


Ordering Information
Cat# Product Name Size 1839 MagMAX-96 for Microarrays Total RNA Isolation Kit 96 purifications 1964 RNaseAlert Lab Test Kit 25 rxns 1966 RNaseAlert QC System 5 x 96 assays 1982 MELT Total Nucleic Acid Isolation System 50 purifications 2682 RNase Inhibitor (Cloned) 40 U/l 2500 U 2684 RNase Inhibitor (Cloned) 40 U/l 10,000 U 9738 TRI Reagent 100 ml
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Protector RNase Inhibitor
2. Protector RNase Inhibitor Enhance the Protection of RNA against Degradation
3. RNase Protection Assay (RPA) Using DIG-Labeled RNA Probes
4. Inducing RNAi with siRNA Cocktails Generated by RNase III
5. Dicer vs. RNase III for Preparation of siRNA Cocktails
6. Combat RNase Contamination in the Lab
7. Measuring RNase Activity A Real-time Kinetic Analysis
8. Increase Protein Activity and Yield
9. Reagents for Up and Down-Regulation of miRNA Activity in Mammalian Cells
10. Translate More Protein with Higher Biological Activity
11. Detection of Reporter Gene Activity in Cell Cultures and Murine Epidermis After Helios Gene Gun-Mediated Particle Bombardment
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:2/10/2016)... , Feb. 10, 2016 NX Prenatal Inc., ... proprietary NeXosome® technology for early warning of adverse ... most recent study by Dr. Thomas McElrath ... Society for Maternal Fetal Medicine,s (SMFM) annual meeting held ... th , 2016.  The presentation reported initial positive ...
(Date:2/10/2016)... RICHLAND, Wash., Feb. 10, 2016  IsoRay, Inc. (NYSE ... seed brachytherapy and medical radioisotope applications for the treatment ... cancers, today announced its financial results for the second ... December 31, 2015. --> ... second quarter of fiscal 2016, which ended December 31, ...
(Date:2/10/2016)... ... February 10, 2016 , ... Benchmark Research, a fully-integrated ... of two long-standing principal investigators (PI) to the roles of Chief Medical Officer, ... , Dr. Laurence Chu, a Benchmark Research PI in the Austin office, will ...
(Date:2/10/2016)... ... February 10, 2016 , ... ... their comprehensive training and support program, Sonalinkā„¢ remote monitoring. The inaugural launch of ... on Friday, February 5th, connecting Dr. Samuel Peretsman to a HIFU technical expert ...
Breaking Biology Technology:
(Date:1/20/2016)... MINNETONKA, Minn. , Jan. 20, 2016   ... that supports the entire spectrum of clinical research, is ... in 2015. MedNet,s significant achievements are the result of ... of) iMedNet eClinical , it,s comprehensive, ... --> --> Key MedNet growth ...
(Date:1/15/2016)... , Jan. 15, 2016 Recent publicized breaches ... to find new ways to ensure data security and ... iOS and Android that ties a ... transforming it into a hardware authorization token. Customer service ... their fingerprint on their KodeKey enabled device to verify ...
(Date:1/11/2016)... Jan. 11, 2016 Synaptics Incorporated (NASDAQ: ... today announced that its ClearPad ® TouchView ™ ... won two separate categories in the 8 th ... Best Technology Breakthrough. The Synaptics ® TDDI solution ... supply chain, thinner devices, brighter displays and borderless designs. ...
Breaking Biology News(10 mins):