Navigation Links
RNase Activity in Mouse Tissue: Classification, Hierarchy, and Methods for Control

Protecting RNA During Isolation
RNase activity varies dramatically across different tissues. A comparison of total RNase activities for 8 different mouse tissues showed that total RNase activity spans a 181,000-fold range from pancreas to brain (Figure 1), which highlights the level of RNase control required for successful RNA isolation.

Figure 1. Quantitative Hierarchy of RNase Activity in Mouse Tissues. Mouse tissues were dounced in non-denaturing, neutral pH buffer (50 mM NaCl and 1 mM Mg2+), and total RNase activity was measured using Ambions fluorescence-based RNaseAlert QC System. Lysates were treated with EDTA (5 mM) or Ambions RNase Inhibitor protein (40 U/100 l reaction).

The tissues profiled for intrinsic RNase activity belonged in one of two groups. While RNases in Group A tissues were insensitiveto EDTA (and thus, Mg2+ independent), these tissues contained very high levels of RNase activity that were effectively inhibited by Ambions recombinant RNase Inhibitor, an inhibitor of RNase A-type enzymes. Group B tissues exhibited much lower levels of RNase activity that were more thoroughly inhibited by EDTA than by Ambions RNase Inhibitor (both before and after heating the lysates to inactivate endogenous inhibitors). Because RNase A enzyme activity is unaffected by the addition of EDTA, whereas other RNases are usually EDTA-sensitive, these results suggest that the RNas e A family of enzymes are the source of RNase activity in Group A tissues, whereas a more functionally heterogeneous group of RNases are active in Group B tissues.

For RNA isolation from tissues containing very high levels of RNase, we recommend TRI Reagent (see RNA, DNA, and Protein from a Single Sample and High Throughput RNA Isolation for High Performance Microarray Analysis). For tissues exhibiting more typical levels of RNase activity, Ambions convenient hands-free MELT Total RNA Isolation System maximizes yields with minimal handling (see Watch Your Tissue MELT into High Quality Total RNA).


Scientific Contributors

Julie Krosting, Gary Latham Ambion, Inc.


back to top


Ordering Information
Cat# Product Name Size 1839 MagMAX-96 for Microarrays Total RNA Isolation Kit 96 purifications 1964 RNaseAlert Lab Test Kit 25 rxns 1966 RNaseAlert QC System 5 x 96 assays 1982 MELT Total Nucleic Acid Isolation System 50 purifications 2682 RNase Inhibitor (Cloned) 40 U/l 2500 U 2684 RNase Inhibitor (Cloned) 40 U/l 10,000 U 9738 TRI Reagent 100 ml
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Protector RNase Inhibitor
2. Protector RNase Inhibitor Enhance the Protection of RNA against Degradation
3. RNase Protection Assay (RPA) Using DIG-Labeled RNA Probes
4. Inducing RNAi with siRNA Cocktails Generated by RNase III
5. Dicer vs. RNase III for Preparation of siRNA Cocktails
6. Combat RNase Contamination in the Lab
7. Measuring RNase Activity A Real-time Kinetic Analysis
8. Increase Protein Activity and Yield
9. Reagents for Up and Down-Regulation of miRNA Activity in Mammalian Cells
10. Translate More Protein with Higher Biological Activity
11. Detection of Reporter Gene Activity in Cell Cultures and Murine Epidermis After Helios Gene Gun-Mediated Particle Bombardment
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:10/9/2017)... ... October 09, 2017 , ... At its national board meeting in ... professor in Harvard University’s Departments of Physics and Astronomy, has been selected for membership ... the winning team for the 2015 Breakthrough Prize in Fundamental physics for the discovery ...
(Date:10/9/2017)... ... October 09, 2017 , ... ... October 5, 2017, in the medical journal, Epilepsia, Brain Sentinel’s SPEAC® System ... standard, video EEG, in detecting generalized tonic-clonic seizures (GTCS) using surface electromyography ...
(Date:10/7/2017)... ... October 06, 2017 , ... ... Hi-C-based genomic technologies, launched its ProxiMeta™ Hi-C metagenome deconvolution product, featuring the ... and accompanying cloud-based bioinformatics software to perform Hi-C metagenome deconvolution using their ...
(Date:10/6/2017)... ... October 06, 2017 , ... The ... and technology sector at their fourth annual Conference where founders, investors, innovative practitioners ... and the ELEVATE pitch competition showcasing early stage digital health and med tech ...
Breaking Biology Technology:
(Date:4/17/2017)... , April 17, 2017 NXT-ID, Inc. (NASDAQ: ... announces the filing of its 2016 Annual Report on Form 10-K ... Commission. ... 10-K is available in the Investor Relations section of the Company,s ... the SEC,s website at http://www.sec.gov . 2016 Year ...
(Date:4/13/2017)... PUNE, India , April 13, 2017 According ... Identity Proofing, Identity Authentication, Identity Analytics, Identity Administration, and Authorization), Service, Authentication ... by MarketsandMarkets™, the IAM Market is expected to grow from USD 14.30 ... Annual Growth Rate (CAGR) of 17.3%. ... ...
(Date:4/11/2017)... Research and Markets has announced the addition of the ... ... to grow at a CAGR of 30.37% during the period 2017-2021. ... been prepared based on an in-depth market analysis with inputs from ... prospects over the coming years. The report also includes a discussion ...
Breaking Biology News(10 mins):