Navigation Links
RNase Activity in Mouse Tissue: Classification, Hierarchy, and Methods for Control

Protecting RNA During Isolation
RNase activity varies dramatically across different tissues. A comparison of total RNase activities for 8 different mouse tissues showed that total RNase activity spans a 181,000-fold range from pancreas to brain (Figure 1), which highlights the level of RNase control required for successful RNA isolation.

Figure 1. Quantitative Hierarchy of RNase Activity in Mouse Tissues. Mouse tissues were dounced in non-denaturing, neutral pH buffer (50 mM NaCl and 1 mM Mg2+), and total RNase activity was measured using Ambions fluorescence-based RNaseAlert QC System. Lysates were treated with EDTA (5 mM) or Ambions RNase Inhibitor protein (40 U/100 l reaction).

The tissues profiled for intrinsic RNase activity belonged in one of two groups. While RNases in Group A tissues were insensitiveto EDTA (and thus, Mg2+ independent), these tissues contained very high levels of RNase activity that were effectively inhibited by Ambions recombinant RNase Inhibitor, an inhibitor of RNase A-type enzymes. Group B tissues exhibited much lower levels of RNase activity that were more thoroughly inhibited by EDTA than by Ambions RNase Inhibitor (both before and after heating the lysates to inactivate endogenous inhibitors). Because RNase A enzyme activity is unaffected by the addition of EDTA, whereas other RNases are usually EDTA-sensitive, these results suggest that the RNase A family of enzymes are the source of RNase activity in Group A tissues, whereas a more functionally heterogeneous group of RNases are active in Group B tissues.

For RNA isolation from tissues containing very high levels of RNase, we recommend TRI Reagent (see RNA, DNA, and Protein from a Single Sample and High Throughput RNA Isolation for High Performance Microarray Analysis). For tissues exhibiting more typical levels of RNase activity, Ambions convenient hands-free MELT Total RNA Isolation System maximizes yields with minimal handling (see Watch Your Tissue MELT into High Quality Total RNA).


Scientific Contributors

Julie Krosting, Gary Latham Ambion, Inc.


back to top


Ordering Information
Cat# Product Name Size 1839 MagMAX-96 for Microarrays Total RNA Isolation Kit 96 purifications 1964 RNaseAlert Lab Test Kit 25 rxns 1966 RNaseAlert QC System 5 x 96 assays 1982 MELT Total Nucleic Acid Isolation System 50 purifications 2682 RNase Inhibitor (Cloned) 40 U/l 2500 U 2684 RNase Inhibitor (Cloned) 40 U/l 10,000 U 9738 TRI Reagent 100 ml
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Protector RNase Inhibitor
2. Protector RNase Inhibitor Enhance the Protection of RNA against Degradation
3. RNase Protection Assay (RPA) Using DIG-Labeled RNA Probes
4. Inducing RNAi with siRNA Cocktails Generated by RNase III
5. Dicer vs. RNase III for Preparation of siRNA Cocktails
6. Combat RNase Contamination in the Lab
7. Measuring RNase Activity A Real-time Kinetic Analysis
8. Increase Protein Activity and Yield
9. Reagents for Up and Down-Regulation of miRNA Activity in Mammalian Cells
10. Translate More Protein with Higher Biological Activity
11. Detection of Reporter Gene Activity in Cell Cultures and Murine Epidermis After Helios Gene Gun-Mediated Particle Bombardment
Post Your Comments:
(Date:12/24/2014)... , Dec. 24, 2014   BioLife Solutions ... developer, manufacturer and marketer of proprietary clinical grade ... and precision thermal shipping products for cells ... it will hold its 2015 Annual Meeting of Stockholders ... Because the expected date for the Annual Meeting represents ...
(Date:12/24/2014)... MD (PRWEB) December 23, 2014 ... into law H.R. 83, the Omnibus and Continuing ... first time, made hydrocephalus a condition eligible to ... Programs (CDMRP) administered by the Department of Defense ... with its allies on Capitol Hill, is celebrating ...
(Date:12/24/2014)... Diego, CA (PRWEB) December 23, 2014 ... where their Twitter followers can submit their #HolidayInTheLab ... themselves and their lab mates. , For those struggling ... lab mates, the Pipette.com Holiday in the Lab Contest ... Pipette.com Twitter followers to send pictures of ...
(Date:12/24/2014)... Fla. , Dec. 23, 2014  Rock Creek ... that it has filed a Clinical Trial Application (CTA) ... Healthcare products Regulatory Agency (MHRA) seeking regulatory approval to ... lead molecule, Anatabine Citrate. Contingent on the ... conduct a Phase I trial to assess the safety, ...
Breaking Biology Technology:BioLife Solutions Sets Date for Annual Meeting of Stockholders 2Rare Brain Condition Now Eligible for Department of Defense Research Dollars 2Major Pipette Distributor Pipette.com Announces their Holiday in the Lab Contest 2Rock Creek Pharmaceuticals Files Clinical Trial Application in the UK for Clinical Trials of Anatabine Citrate Formulations 2Rock Creek Pharmaceuticals Files Clinical Trial Application in the UK for Clinical Trials of Anatabine Citrate Formulations 3
... , HOPKINTON, Mass., Dec. 3 Caliper ... of tools and services for drug discovery and life sciences ... vivo imaging systems. Living Image software drives the more than ... Image 4.0 features advanced spectral unmixing tools to enhance fluorescent ...
... , MANILA, Philippines, Dec. 2 Spectrum Blue Steel ... companies on the planet. Through the efforts of True Green ... Leroy Johnston, who has been in the recycling business for ... years ahead of everyone and is setting the stage for ...
... , NEW YORK, Dec. 2 ... available in its catalogue: , Drug Discovery Technologies ... , http://www.reportlinker.com/p0164246/Drug-Discovery-Technologies---A-Global-Update-of-Market-Trends--Opportunities.html , Drug discovery has ... company,s business strategy. There are a number of new ...
Cached Biology Technology:Caliper Life Sciences Launches Living Image(R) 4.0 Software 2Spectrum Blue Steel partners with Famous Chemists for Procuring Profitable Applications from Garbage Using the Biosphere MKV and Electrostatic Precipitators 2Spectrum Blue Steel partners with Famous Chemists for Procuring Profitable Applications from Garbage Using the Biosphere MKV and Electrostatic Precipitators 3Reportlinker Adds Drug Discovery Technologies - A Global Update of Market Trends & Opportunities 2Reportlinker Adds Drug Discovery Technologies - A Global Update of Market Trends & Opportunities 3Reportlinker Adds Drug Discovery Technologies - A Global Update of Market Trends & Opportunities 4Reportlinker Adds Drug Discovery Technologies - A Global Update of Market Trends & Opportunities 5Reportlinker Adds Drug Discovery Technologies - A Global Update of Market Trends & Opportunities 6Reportlinker Adds Drug Discovery Technologies - A Global Update of Market Trends & Opportunities 7Reportlinker Adds Drug Discovery Technologies - A Global Update of Market Trends & Opportunities 8Reportlinker Adds Drug Discovery Technologies - A Global Update of Market Trends & Opportunities 9
(Date:12/22/2014)... -- NXT-ID, Inc. (NASDAQ: NXTD and NXTDW) ... on the growing mobile commerce market, announces it has ... Robbins for the 2015 International CES debut of ... Apollo Robbins will be at the NXT-ID booth ... of his famous theft techniques to visiting media and ...
(Date:12/19/2014)... 18, 2014  23andMe, Inc., the leading personal genetics ... pinpoints fine-scale differences in genetic ancestry of individuals from ... Since immigrants first arrived more than four hundred ... has served as a meeting place for peoples from ... the ongoing mixing of peoples with African, European, and ...
(Date:12/17/2014)... 16, 2014 Valencell, a leader in performance ... technology to industry leaders such as Intel, Jabra, Atlas, ... biometric wearable products. These products will be showcased at ... Las Vegas . "Our partners ... and robust – with the clinical data to back ...
Breaking Biology News(10 mins):NXT-ID Hires Master Pickpocket and Security Consultant Apollo Robbins for Wocket Smart Wallet CES 2015 Debut 2NXT-ID Hires Master Pickpocket and Security Consultant Apollo Robbins for Wocket Smart Wallet CES 2015 Debut 3NXT-ID Hires Master Pickpocket and Security Consultant Apollo Robbins for Wocket Smart Wallet CES 2015 Debut 423andMe Study Sketches Genetic Portrait of the United States 223andMe Study Sketches Genetic Portrait of the United States 323andMe Study Sketches Genetic Portrait of the United States 4Valencell PerformTek Biometrics Power the Most Accurate Wearables at CES 2015 2
... SALT LAKE CITYResearchers have discovered two enzymes that, when ... cancer. Manipulating these enzymes genetically might lead to targeted ... tumors. "We could conceivably reactivate a completely normal ... could prevent the growth of a tumor if reactivated," ...
... crimefighter who protected the innocent from pirates, hijackers and ... its costumed namesake, this electromagnetic phantoma carbon and polymer ... the National Institute of Standards and Technology (NIST) for ... The NIST phantom serves as a mannequin in a ...
... Researchers at The University of Texas School of Public Health ... of community-acquired antibiotic-resistant Staph ylococcus aureus ... (PVL). The Panton Valentine leukocidin is made ... is typically produced by community-acquired methicillin-resistant S. aureus ...
Cached Biology News:Newly found enzymes may play early role in cancer 2Electromagnetic phantom exorcises specters of metal detector tests 2UT Public Health researchers find link to severe Staph infections 2
5 minutes from gel band to purified DNA...
...
...
Rabbit polyclonal to Staphylococcus Enterotoxin E (Biotin) ( Abpromise for all tested applications)....
Biology Products: