Navigation Links
RNArticles: RNA Amplification Used to Develop a New In Vitro HCMV Assay

href="http://www.ambion.com/catalog/CatNum.php?1918">MicroPoly(A)Pure Kit. cDNA probes were made by reverse transcription of the mRNA (for fibroblasts*) or amplified RNA (for CD34+ cells).

While all of the genes expressed in an active viral infection were observed with the fibroblasts during the complete course of viral replication (Figure 1, Panel A), the CD34+ cells showed expression of only a subset of viral genes during the 8 day time course following infection (Figure 1, Panel B). (Reactivation of viral replication was achieved in the CD34+ cells by
co-culturing with fibroblasts.) Analysis of the gene expression microarray data obtained from the latent CD34+ cells was validated by RT-PCR analysis. Thus the subset of genes expressed in the absence of active viral replication could be indicative of the subset of genes that are necessary for latent infection.

Figure 1. Comparison of HCMV Gene Expression in CD34+ and Primary Human Fibroblasts. (A) HCMV arrays were analyzed using cDNA probes made against primary human fibroblasts. (B) HCMV arrays were analyzed using cDNA probes against CD34+ cells. Each of the arrays has viral sequences spotted in triplicate, the bottom right most triplicate is spotted with cellular housekeeping genes as controls.


*Note: For the human fibroblast RNA, the gene expression profile did not vary significantly between amplified vs. unamplified samples.

We would like to thank Dr. Felicia Goodrum for pr
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. RNArticles: Keeping RNA Intact Prior to RNA Isolation - RNAlater
2. prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification
3. Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels
4. Greater Amplification Specificity with New Hot Start PCR Enzyme
5. Enhanced Amplification of Long Targets with PfuTurbo DNA Polymerase
6. Optimizing pfuturbo DNA Polymerase Amplification Reactions with Perfect Match PCR Enhancer
7. Improve Amplification Specificity with Hot Start PCR Enzyme
8. Amplification of the amoA Gene of Nitrosococcus oceani Bacteria Using Eppendorf MasterTaq
9. Amplification of Mouse cDNAs for Microarrays Using the Eppendorf MasterTaq Kit
10. Optimizing the Direct Amplification of Missing cDNA 5 Ends Using the Eppendorf Mastercycler gradient
11. Optimizing DNA Amplification Protocols using the Eppendorf Mastercycler
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: RNArticles RNA Amplification Used Develop New Vitro HCMV Assay

(Date:4/16/2015)... Capillus, LLC announces plans to ... in Europe and Asia. The Miami-based medical device ... former Business Development Director of Svenson Group Europe, ... Bermúdez’s professional achievements include increasing the number of ... penetrating the Portuguese marketplace with centers in Lisbon ...
(Date:4/16/2015)... -- Schulman Associates IRB, Inc., the industry-leading central IRB in ... on Laboratory Drive in the heart of ... will house review board and service staff, which will ... customer service to clients. CBRE | ... deal, while Capital Associates Management, LLC represented the landlord.   ...
(Date:4/16/2015)... 2015 According to the 2014 ... outside source was ranked first among the leading ... to lifting, pushing, pulling, holding, carrying, or throwing ... The David Round Company takes statistics like this ... hard to develop and design equipment that prevents ...
(Date:4/16/2015)... Ames, IA (PRWEB) April 16, 2015 ... (CAST) announces that the prestigious 2015 Borlaug CAST ... honored faculty member at Tuskegee University in Alabama. ... by written material; public presentations; and/or the use ... excels in all three areas. As a specialist ...
Breaking Biology Technology:Capillus, LLC Extends Laser Hair Therapy Products to Europe and Asia 2Schulman IRB to Open Office in Research Triangle Park, N.C. 2The David Round Company Helps Companies Meet OSHA Standards by Combining Safety, Ergonomics, and Productivity 2The 2015 Borlaug CAST Communication Award Goes to Channapatna Prakash 2
... Oct. 20 Academia, science, industry,and government leaders ... at the Dedication and Open House celebration for ... Nutrition Research Building and the NC,State Fruit and ... is home to NC A&T University, NC Central ...
... GlaxoSmithKline plc (NYSE: GSK ) will announce its ... 30, 2008. The,announcement is scheduled for release to the ... At 10:00 am EDT, media are invited to ... Andrew Witty, Chief Executive Officer,and Julian Heslop, Chief Financial ...
... Branding joins Pierpoint International as Global VP of Recruitment Operations and ... ... 2008 -- Pierpoint International is pleased to announce the appointment of ... Cantu, most recently served as AVP of Staffing and Diversity for ...
Cached Biology Technology:Campus Celebrates Historic Milestone 2Campus Celebrates Historic Milestone 3Campus Celebrates Historic Milestone 4Campus Celebrates Historic Milestone 5Robert Cantu Appointed Vice President of Pierpoint International 2
(Date:3/30/2015)... Mar. 26, 2015 Research and Markets ... "Global Gesture Recognition Market in Automotive Sector 2014-2018" ... forecast the Global Gesture Recognition market in Automotive Sector ... period 2013-2018. Gesture recognition is the ability ... gestures of an individual. Gesture recognition technology can be ...
(Date:3/24/2015)...   NexID Biometrics LLC, whose spoof-mitigation technology ... announced the beginning of shipments of version 2.0 of ... company, based in Potsdam, N.Y. , ... which began here today at the Walter E. Washington ... its SDK boosts the accuracy rate range to 96.5 ...
(Date:3/23/2015)... , March 23, 2015  In the 2014 ... fraud case conviction losses. This figure accounts for a ... according to The Nilson Report, exceeds $11 billion globally. ... tool after a crime has occurred. Investor Mark ... polygraph expert Joe Paolella partnered to administer ...
Breaking Biology News(10 mins):Global Gesture Recognition Market in Automotive Sector Report 2015 - Growing Number of Partnerships 2NexID Begins Shipments Of Version 2.0 Fake-Finger-Detection Solution 2Polygraph Test Now Used to Protect Investors From Fraud 2Polygraph Test Now Used to Protect Investors From Fraud 3
... has allowed social spiders to stretch the laws of nature ... The findings, published in this week,s edition of the ... explain why social spiders thrive in tropical areas but dwindle ... tends to be constrained by a scaling principle scientists call ...
... delivers a comprehensive next-generation identity management platform, ... ... 5 Sun Microsystems, Inc.,and Aware, Inc. (Nasdaq: ... the integration of Aware,s Biometric Services Platform,(BioSP(TM)) with Sun ...
... deforestation threatens the world,s tropical forests but offers ... coauthored by William Laurance of the Smithsonian Tropical ... Tropical Forests" will be featured in the September ... Ecology & Evolution ., Rhett Butler of ...
Cached Biology News:Spiders who eat together, stay together: UBC research 2Sun Microsystems and Aware, Inc. Announce Integration of Aware's BioSP with Sun Identity Management Solutions 2Sun Microsystems and Aware, Inc. Announce Integration of Aware's BioSP with Sun Identity Management Solutions 3Sun Microsystems and Aware, Inc. Announce Integration of Aware's BioSP with Sun Identity Management Solutions 4Sun Microsystems and Aware, Inc. Announce Integration of Aware's BioSP with Sun Identity Management Solutions 5The drivers of tropical deforestation are changing, say scientists 2
MagSi monodispers, magnetic silica particles with modified surface for SO3 coupling chemistry....
Anti-Chicken IgY, (IgG) F(ab')2 Fragment Specific....
... Polyclonal Antibody to Asb-2 (Ankyrin repeat and ... raised against synthetic peptides containing amino acids ... Human spleen lysate (Imgenex Catalog No. 40149) ... It should cross react with mouse and ...
... polyclonal to ErbB 2 - BSA ... for all tested applications). ... conjugated to KLH, corresponding to C ... c-ErbB2 Entrez GeneID: ...
Biology Products: