Navigation Links
RNArticles: RNA Amplification Used to Develop a New In Vitro HCMV Assay

href="http://www.ambion.com/catalog/CatNum.php?1918">MicroPoly(A)Pure Kit. cDNA probes were made by reverse transcription of the mRNA (for fibroblasts*) or amplified RNA (for CD34+ cells).

While all of the genes expressed in an active viral infection were observed with the fibroblasts during the complete course of viral replication (Figure 1, Panel A), the CD34+ cells showed expression of only a subset of viral genes during the 8 day time course following infection (Figure 1, Panel B). (Reactivation of viral replication was achieved in the CD34+ cells by
co-culturing with fibroblasts.) Analysis of the gene expression microarray data obtained from the latent CD34+ cells was validated by RT-PCR analysis. Thus the subset of genes expressed in the absence of active viral replication could be indicative of the subset of genes that are necessary for latent infection.

Figure 1. Comparison of HCMV Gene Expression in CD34+ and Primary Human Fibroblasts. (A) HCMV arrays were analyzed using cDNA probes made against primary human fibroblasts. (B) HCMV arrays were analyzed using cDNA probes against CD34+ cells. Each of the arrays has viral sequences spotted in triplicate, the bottom right most triplicate is spotted with cellular housekeeping genes as controls.


*Note: For the human fibroblast RNA, the gene expression profile did not vary significantly between amplified vs. unamplified samples.

We would like to thank Dr. Felicia Goodrum for pr
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. RNArticles: Keeping RNA Intact Prior to RNA Isolation - RNAlater
2. prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification
3. Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels
4. Greater Amplification Specificity with New Hot Start PCR Enzyme
5. Enhanced Amplification of Long Targets with PfuTurbo DNA Polymerase
6. Optimizing pfuturbo DNA Polymerase Amplification Reactions with Perfect Match PCR Enhancer
7. Improve Amplification Specificity with Hot Start PCR Enzyme
8. Amplification of the amoA Gene of Nitrosococcus oceani Bacteria Using Eppendorf MasterTaq
9. Amplification of Mouse cDNAs for Microarrays Using the Eppendorf MasterTaq Kit
10. Optimizing the Direct Amplification of Missing cDNA 5 Ends Using the Eppendorf Mastercycler gradient
11. Optimizing DNA Amplification Protocols using the Eppendorf Mastercycler
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: RNArticles RNA Amplification Used Develop New Vitro HCMV Assay

(Date:5/15/2008)...Calif., May 15 /- NeurogesX...ny focused on developing novel,pain management the...esent,on Monday, May 19, 2008 at the Rodman & Rens...ny DiTonno, President and Chief Executive,Officer,...inancial Officer,will be available to respond to ...
(Date:5/15/2008)... May 15 /- Escalon Medical ... its fiscal third quarter and,nine months ended Ma...08, product revenue increased 18% to,$8,139,000 co... last fiscal,year. Product revenues were driven by... units., Net revenue for the third quarter of fis...
(Date:5/15/2008)...d BARCELONA, Spain, May 15 /PRNewswire-FirstCall/ ...boratorios Almirall, S.A. (ALM.MC),will host a web...e-clinical,data findings presented at the American...ich will include a slide presentation and a Q&A se...30am New York time, 3:30pm CET,2:30pm UKT., (Log...
(Date:5/15/2008)...ncides with the ,60s cleanup of toxic pollution in...ton, SEATTLE, May 15 /- Evolution is..., at least in the case of a little fish called the...ative warp-speed reverse,according to a study led...,Center and published online today ahead of print ...
Breaking Biology Technology:NeurogesX to Present at the Rodman & Renshaw 5th Annual Global Healthcare Conference 2NeurogesX to Present at the Rodman & Renshaw 5th Annual Global Healthcare Conference 3Escalon(R) Reports Third Quarter Fiscal 2008 Results 2Escalon(R) Reports Third Quarter Fiscal 2008 Results 3Escalon(R) Reports Third Quarter Fiscal 2008 Results 4Escalon(R) Reports Third Quarter Fiscal 2008 Results 5Escalon(R) Reports Third Quarter Fiscal 2008 Results 6Escalon(R) Reports Third Quarter Fiscal 2008 Results 7Escalon(R) Reports Third Quarter Fiscal 2008 Results 8Forest Laboratories, Inc. and Laboratorios Almirall, S.A. to Host Webcast to Discuss Aclidinium Bromide Data Presentations at the American Thoracic Society Annual Congress 2Researchers Document Rapid, Dramatic 'Reverse Evolution' in the Threespine Stickleback Fish 2Researchers Document Rapid, Dramatic 'Reverse Evolution' in the Threespine Stickleback Fish 3Researchers Document Rapid, Dramatic 'Reverse Evolution' in the Threespine Stickleback Fish 4
...rinceton, Wis. - The newest online tool for fishi... big one," but it might help you locate some promi...b application featured on Lake-Link.com integrat...es with global positioning information to provide ...ations. The application requires only an Internet ...
...adison, Wis. - Research into the genetic underpin...nks in part to technology from a local biotechnolo...ashington and the Howard Hughes Medical Institut...tion, documented in a study published online in N...ed by a large deletion that spans six genes on chr...
...In the last couple years, many organizations tried... infrastructure. Rather than spending money to upg...ey had in place. Has that hesitation to upgrade te...iveness? I believe it has. , ,There is a continual... cycle, you move from competitive advantage to com...
...ilwaukee, Wis. - A research consortia comprised o...f lithium ion battery technology for hybrid-electr...ry power in low temperatures, and it is counting o...ht chemistry. , ,The U.S. Advanced Battery Consor...ge technologies operating under the FreedomCAR an...
Other Biology Technology:Online fishing site introduces interactive maps 2Online fishing site introduces interactive maps 3NimbleGen microarrays speed genetic findings 2Technology cycles from advantage to necessity to disadvantage 2Technology cycles from advantage to necessity to disadvantage 3Johnson Controls partnership wins new contract 2Johnson Controls partnership wins new contract 3
(Date:5/15/2008)...d nearly 150 years ago, bird beaks are exquisitely... MIT mathematicians and engineers has now explaine... beaks to defy gravity and transport food into the...stern North America, takes advantage of surface in...propel bits of food from the tip of its long beak ...
(Date:5/15/2008)...ng-held assumptions, high-salt diets may not incre...from the Albert Einstein College of Medicine of Ye...ter examining dietary intake among a nationally re...tein researchers actually observed a significantly...se (CVD) associated with lower sodium diets. They ...
(Date:5/15/2008)...oved the power output of one type of fuel cell by ... help these environmentally friendly energy storag... in portable electronics. , The new material key...han its conventional industrial counterpart, among...itional fuel-cell membranes with these cost-effect...
(Date:5/15/2008)...ling-Hampton, Ph.D., Managing Director of Molecula...r, collaborated with colleagues from around the wo...ions in chick and mouse model systems also play an...vertebral abnormalities. , The discovery was pu...nal of Human Genetics. , Working with samples f...
Breaking Biology News(10 mins):MIT solves gravity-defying bird beak mystery 2MIT solves gravity-defying bird beak mystery 3New study casts further doubt on risk of death from higher salt intake 2MIT creates new material for fuel cells 2MIT creates new material for fuel cells 3Stowers Institute researchers identify gene linked to vertebral defects in patient populations 2Mayo Clinic Proceedings examines link between bacteria in the digestive system and obesity 15719 1Mayo Clinic Proceedings examines link between bacteria in the digestive system and obesity 15719 2Mayo Clinic Proceedings examines link between bacteria in the digestive system and obesity 15719 3Occupational Therapy Month 2008 Highlights Profession as Career Choice 15716 1Occupational Therapy Month 2008 Highlights Profession as Career Choice 15716 2Susan G Komen for the Cure 28C 29 Opens Registration For 19th Annual Susan G Komen National Race for the Cure 28C 29 15713 1Susan G Komen for the Cure 28C 29 Opens Registration For 19th Annual Susan G Komen National Race for the Cure 28C 29 15713 2Susan G Komen for the Cure 28C 29 Opens Registration For 19th Annual Susan G Komen National Race for the Cure 28C 29 15713 3Susan G Komen for the Cure 28C 29 Opens Registration For 19th Annual Susan G Komen National Race for the Cure 28C 29 15713 4UHW Outlines Solutions to Californias Health Crisis 15710 1
...robes built by researchers at Duke,s Pratt School ...ogs. The engineers said their demonstration showed... during human endoscopic surgeries in which operat...s. , If the probes prove beneficial in human testi...er endoscopic surgeries, said the Duke engineers. ...
...ng cocaine –?made with inexpensive, off-the-shelf ...archers at the University of California, Santa Bar...aureate participated in the discovery. The potenti... include bioterrorism detection and important medi...n sensors and collected data shown in a graph in t...
... active antiretroviral therapy (HAART) is very cos...the study also shows that reimbursement to physici...threatening patients, access to care. The study fi...al Infectious Diseases, now available online. , Wh... and death of patients with HIV, it does so at a p...
...(cartilage covering the ends of bones where they m... illness, and can lead to degenerative disease. Tr...s articular cartilage have achieved limited result...re this type of injured cartilage. Tissue engineer... to the injured site is a promising new approach t...
Other Biology News:3D ultrasound device poised to advance minimally invasive surgery 23D ultrasound device poised to advance minimally invasive surgery 3Portable cocaine sensor developed at UC Santa Barbara 2Portable cocaine sensor developed at UC Santa Barbara 3Study shows AIDS drugs cost-effective, care underfunded 2Study shows AIDS drugs cost-effective, care underfunded 3Stem cells from muscles can repair cartilage 2Stem cells from muscles can repair cartilage 3
DETACHaBEAD Mouse CD4 from Dynal Biotech
Antibodies were affinity purified using epitopes specific to USP33 immobilized on solid support.
10% Normal Goat Serum from Invitrogen
This monoclonal antibody recognizes 30% of IgM-rheumatoid factor paraproteins from unrelated individuals. It does not cross-react with IgM, IgA, or IgG proteins lacking rheumatoid factor nor with p
Biology Products: