( tissues in RNA later can ...)RNArticles: Keeping RNA Intact Prior to RNA Isolation - RNAlater

tissues in RNAlater can be stored at room temperature, allowing for easy integration of this collection procedure into complex autopsy protocols. The tissue is also easily transported, thereby facilitating interinstitutional collaborations.

Fine Dissection of Tissue Samples for RNA Isolation
Rodrigo MC, Martin DS, Redetzke RA, Eyster KM (2002) A method for the extraction of high-quality RNA and protein from single small samples of arteries and veins preserved in RNAlater. J Pharmacol Toxicol Methods 47: 87-92.

The veins and arteries comprising the mesenteric vasculature play a critical role in cardiovascular homeostasis. It is therefore of interest to examine gene expression in these vessels for various cardiovascular studies. For such studies it is important to separate veins from arteries (and to remove contaminating adipose, nerve and connective tissue) since they function differently under normal conditions and respond differently to drugs. The time required for such intricate dissection and the high levels of RNases in these tissues generally results in RNA degradation. Fortunately, Rodrigo et al. report the prevention of RNA degradation in rat mesenteric vasculature following dissection and storage of the mesenteric arcade in RNAlater. Coarsely dissected tissue was stored at -20C in RNAlater for a minimum of 3 days. Storage resulted in a stiffening of the
'"/>

Source:

Page: All 1 2 3 4 5 6 7
Related biology technology :

1. RNArticles: RNA Amplification Used to Develop a New In Vitro HCMV Assay
2. Comparison of PCR Kleen Spin Columns to Traditional Methods for Purification of PCR Products Prior to Sequencing, Rev A
3. Fast and Easy Isolation of PCR-Ready Genomic DNA from Whole Blood
4. High-Throughput Isolation of Total RNA
5. Simple Isolation of RNA from Tissue and Cultured Cells
6. Fast Isolation of Total RNA from Small Numbers of Cells
7. Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit
8. Isolation of Microorganisms
9. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
10. Mouse Tail Genomic DNA Isolation Protocol(1)
11. Genomic DNA Isolation Protocol(1,2,3)