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RNArticles: Keeping RNA Intact Prior to RNA Isolation - RNAlater

re to proceed quickly through a difficult collection process. RNAlater not only makes tissue collection easier, but it can actually enable the collection of tissues for RNA isolation that could not previously be subjected to RNA analysis.


Make Tissue Collection Easier by Using the Tissue Storage Solution, RNAlater

Nasal Epithelium Samples
Dreskin SC, Dale SN, Foster SM, Martin D, Buchmeier A, Nelson HS (2002) Measurement of changes in mRNA for IL-5 in noninvasive scrapings of nasal epithelium taken from patients undergoing nasal allergen challenge. J Immunol Methods 268: 189-95.

Scrapings of the nasal epithelium provide a simple, non-invasive way of collecting samples to measure changes in gene expression following allergen challenge. In a study by Dreskin and colleagues, 10 patients were challenged with velvet grass or ragweed extracts, after which 2 scrapings of the nasal epithelium were collected. The collection sticks used for the scrapings were immediately immersed in RNAlater and stored at 4C. Insufficient RNA was obtained from the scrapings of two of the patients. However the remaining patients' samples yielded approximately 150 ng/scraping. The RNA, which resolved into "tight" 18S and 28S ribosomal RNA bands upon gel electrophoresis, was used to analyze IL-5 mRNA levels by RT-PCR. IL-5 mRNA levels were found to correlate with patients' scores of
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