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RNArticles: Keeping RNA Intact Prior to RNA Isolation - RNAlater


RNAlater is a tissue storage solution that stabilizes and protects cellular RNA in fresh tissues. (Ambion's new RNAlater-ICE is designed specifically for use with frozen tissues - see "Stop Grinding Frozen Tissue" for more information.) Tissue harvested and immediately submerged in RNAlater can be stored indefinitely. In doing so, the quality and quantity of the RNA will be maintained, eliminating the need to immediately process or snap-freeze samples.

The first part of this article summarizes findings from three peer reviewed journal articles, each of which cites the use of Ambion's RNAlater and describes how RNAlater can be a real advantage when tissue is difficult to collect or work with. Samples derived from nasal swabs, postmortem tissue, and finely dissected blood vessels are all described. In the second citation, researchers were able to look at protein levels as well as RNA in the tissue. In the third article, the change in sample texture -- in this case stiffening of the blood vessels -- made fine dissection much easier.

In the second part of this article we present another example of how RNAlater can facilitate the tissue collection process when it is complicated by the need for fine dissection or tissue punching. By stabilizing the RNA first, there is no longer any pressu
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