Navigation Links
RNAqueous-96 RNA Isolation Kit Automation Protocol


This protocol was developed for the MultiPROBE II HT Liquid Handling System with a Gripper Integration Platform. Time required: 1 hr 20 min.

For more information on the protocol, see the RNAqueous-96 Instruction Manual (pdf).

  1. Add 150 l Lysis Solution to each well.

  2. Add 150 l 64% ethanol to each well.

  3. Mix and transfer to the 96-well filter plate.

  4. Apply vacuum suction (20 mm Hg) for 3 min (RNA remains bound to the filter).

  5. Add 400 l Wash Solution A to each well and apply vacuum suction (20 mm Hg) for 2 min.

  6. Add 40 l (100 units) DNase I.

  7. Incubate 20 min at room temperature.

  8. Add 400 l Wash Solution B to each well and apply vacuum suction (20 mm Hg) for 2 min.

  9. Add 400 l Wash Solution C/D to each well and apply vacuum suction (20 mm Hg) for 2 min.

  10. Add 400 l 100% ethanol to each well and apply vacuum suction (20 mm Hg) for 2 min.

  11. Move 96-well filter plate to dry heat block covered with fresh paper towels and heat at 42C for 3 min (this removes any residual wash solution).

  12. Move Spacer and Collection plates to the vacuum manifold. Place 96-well filter plate on top.

  13. Add 80 l Elution Buffer to each well.

  14. Apply vacuum suction at 10 mm Hg for 30 sec then at 20 mm Hg for 2 min (RNA is transferred to collection plate).

  15. (optional) Manually check for fluid remaining on filter plate. If fluid is present, centrifuge the filter plate/collection assembly to recover residual sample.


Related Articles
RNAqueous-96 Kit Instruction Manual

High Throughput RNA Purification with Ambion RNAqueous Kits on the Robbins Tango Liquid Handling System (PDF, 136 k)


back to top


Ordering Information
Cat# Product Name Size 1920 RNAqueous-96 Kit 192 purifications
'"/>

Source:


Page: All 1 2

Related biology technology :

1. Fast and Easy Isolation of PCR-Ready Genomic DNA from Whole Blood
2. High-Throughput Isolation of Total RNA
3. Simple Isolation of RNA from Tissue and Cultured Cells
4. Fast Isolation of Total RNA from Small Numbers of Cells
5. Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit
6. Isolation of Microorganisms
7. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
8. Mouse Tail Genomic DNA Isolation Protocol(1)
9. Genomic DNA Isolation Protocol(1,2,3)
10. Basic Plasmid DNA Isolation Protocol
11. Protocol for RNA Isolation Using TRIzol Reagent with Phase Lock Gel-Heavy
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:2/11/2016)... , Germany and ... QGEN ; Frankfurt Prime Standard: QIA) today ... Targeted RNA Panels for gene expression profiling, expanding QIAGEN,s ... (NGS). The panels enable researchers to select from over ... changes and discover interactions between genes, cellular phenotypes and ...
(Date:2/11/2016)... Feb. 11, 2016  Dovetail Genomics™ LLC today announced ... program for a planned metagenomic genome assembly service. ... metagenomic genome assembly method in a talk on Friday, ... & Technology conference in Orlando, Fla. ... complex datasets is difficult. Using its proprietary ...
(Date:2/11/2016)... ... February 11, 2016 , ... Global Stem Cells Group, ... Quito, Ecuador. The new facility will provide advanced protocols and state-of-the-art techniques in ... world. , The new GSCG clinic is headed by four prominent Ecuadorian ...
(Date:2/10/2016)... Feb.10, 2016 ASAE is introducing a hybrid ... Companies (AMC) the option of joining or renewing through ... determined by staff size, every employee in any size ... and reap all available member benefits.   ... organizational membership options will allow organizations of any size ...
Breaking Biology Technology:
(Date:1/20/2016)... Calif. , Jan. 20, 2016  Synaptics ... of human interface solutions, today announced sampling of ... solution for wearables and small screen applications including ... as printers. Supporting round and rectangular shapes, as ... offers excellent performance with moisture on screen, while ...
(Date:1/13/2016)... , January 13, 2016 ... published a new market report titled - Biometric Sensors Market ... Forecast, 2015 - 2023. According to the report, the global biometric ... is anticipated to reach US$1,625.8 mn by 2023, expanding ... In terms of volume, the biometric sensors market is ...
(Date:1/11/2016)...  higi, the leading retail and omni-channel community engagement ... and mobile, today announced it has closed funding ... --> --> ... higi,s health platform – its network of health ... expanding services and programs to retail partners and ...
Breaking Biology News(10 mins):