Navigation Links
RNAqueous-96 RNA Isolation Kit Automation Protocol


This protocol was developed for the MultiPROBE II HT Liquid Handling System with a Gripper Integration Platform. Time required: 1 hr 20 min.

For more information on the protocol, see the RNAqueous-96 Instruction Manual (pdf).

  1. Add 150 l Lysis Solution to each well.

  2. Add 150 l 64% ethanol to each well.

  3. Mix and transfer to the 96-well filter plate.

  4. Apply vacuum suction (20 mm Hg) for 3 min (RNA remains bound to the filter).

  5. Add 400 l Wash Solution A to each well and apply vacuum suction (20 mm Hg) for 2 min.

  6. Add 40 l (100 units) DNase I.

  7. Incubate 20 min at room temperature.

  8. Add 400 l Wash Solution B to each well and apply vacuum suction (20 mm Hg) for 2 min.

  9. Add 400 l Wash Solution C/D to each well and apply vacuum suction (20 mm Hg) for 2 min.

  10. Add 400 l 100% ethanol to each well and apply vacuum suction (20 mm Hg) for 2 min.

  11. Move 96-well filter plate to dry heat block covered with fresh paper towels and heat at 42C for 3 min (this removes any residual wash solution).

  12. Move Spacer and Collection plates to the vacuum manifold. Place 96-well filter plate on top.

  13. Add 80 l Elution Buffer to each well.

  14. Apply vacuum suction at 10 mm Hg for 30 sec then at 20 mm Hg for 2 min (RNA is transferred to collection plate).

  15. (optional)
    '"/>


Source:


Page: All 1 2

Related biology technology :

1. Fast and Easy Isolation of PCR-Ready Genomic DNA from Whole Blood
2. High-Throughput Isolation of Total RNA
3. Simple Isolation of RNA from Tissue and Cultured Cells
4. Fast Isolation of Total RNA from Small Numbers of Cells
5. Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit
6. Isolation of Microorganisms
7. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
8. Mouse Tail Genomic DNA Isolation Protocol(1)
9. Genomic DNA Isolation Protocol(1,2,3)
10. Basic Plasmid DNA Isolation Protocol
11. Protocol for RNA Isolation Using TRIzol Reagent with Phase Lock Gel-Heavy
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: RNAqueous RNA Isolation Kit Automation Protocol

(Date:1/15/2014)... RedBrick Health , a fast-growing leader in consumer ... EmblemHealth , the New York ... the RedBrick Compass health assessment, combined with the RedBrick ... members. EmblemHealth is among the first health plans in ...
(Date:1/15/2014)... 2014 The Microcompetition with Foreign DNA theory ... of these latent viruses is the Epstein Barr Virus (EBV), ... arthritis (RA) is a chronic inflammatory disease that destroys the ... that RA patients have high concentrations of EBV DNA in ...
(Date:1/15/2014)... TaiGen Biotechnology Company, Limited ("TaiGen") today announced that ... leading Russian pharmaceutical company, to develop and commercialize nemonoxacin ... , Turkey and other ... novel antibiotic for the treatment of bacterial infections including ...
(Date:1/14/2014)... Calif. , Jan. 14, 2014 As pet owners drew ... doubt many of them wrote: "Take better care of my furry ... even designer clothes and top-brand carrying cases to take the little ... parents might also feel compelled to buy some pricey toys at ...
Breaking Biology Technology:EmblemHealth Selects RedBrick Health to Power its Next-Generation Health Engagement and Behavior Change System 2EmblemHealth Selects RedBrick Health to Power its Next-Generation Health Engagement and Behavior Change System 3Study: Rheumatoid Arthritis (RA) Patients Have EBV; The CBCD Says this is Consistent with Microcompetition 2Study: Rheumatoid Arthritis (RA) Patients Have EBV; The CBCD Says this is Consistent with Microcompetition 3TaiGen Biotechnology Signed Exclusive License Agreement with R-Pharm for Nemonoxacin (Taigexyn(R)) 2TaiGen Biotechnology Signed Exclusive License Agreement with R-Pharm for Nemonoxacin (Taigexyn(R)) 3TaiGen Biotechnology Signed Exclusive License Agreement with R-Pharm for Nemonoxacin (Taigexyn(R)) 4New Year's Resolution: Give Pets the Gift of Top-Notch Health Care 2
... 16 The landscape of scientific,publications strategy, planning ... guidelines. Half of global publications groups are fully,located ... a shift,to medical groups to increase clinical presentation, ... LLC., In a benchmarking study that included ...
... of international, multi-center registry presented at conference, ... BSX ) today announced that results from ... clinical utility,of the SpyGlass(R) Direct Visualization System will ... from May 17-22 in San Diego,CA. Registry results ...
... Products, L.P. Sponsors Program to Recognize Exemplary Work ... ... 16 CURE magazine announced the winner,and finalists of its ... the Oncology Nursing Society,s (ONS) 33rd Annual,Congress. Ortho Biotech Products, ...
Cached Biology Technology:Industry Executives Finding Value in Shift of Scientific Publications 2Boston Scientific's SpyGlass(R) Direct Visualization System to Be Prominently Featured at Digestive Disease Week(R) 2Boston Scientific's SpyGlass(R) Direct Visualization System to Be Prominently Featured at Digestive Disease Week(R) 3Boston Scientific's SpyGlass(R) Direct Visualization System to Be Prominently Featured at Digestive Disease Week(R) 4Boston Scientific's SpyGlass(R) Direct Visualization System to Be Prominently Featured at Digestive Disease Week(R) 5CURE Magazine Announces Winner of 2008 Extraordinary Healer Award 2
(Date:7/9/2014)... most numerous inhabitants of the sea: tiny haptophytes of ... the naked eye, when they are in bloom in ... even visible on satellite images. "Together with other phytoplankton, ... of the global photosynthesis output," states Prof. Dr. Georg ... the process the greenhouse gas carbon dioxide CO2 ...
(Date:7/8/2014)... 2014 Collectors found the first two specimens of the ... for two decades, the 14-inch-tall plant was identified wrongly as ... after a long search turned up a "pathetic, wilted" ... colleagues identified the spiny plant as a new, possibly endangered ... it was found in Valentine, Texas, population 134 in 2010. ...
(Date:7/8/2014)... greenhouse gases could be vulnerable to price spikes ... released by scholars affiliated with the Energy Institute ... prevent that outcome with three straightforward reforms, the ... Board should consider (1) strengthening the new market,s ... permits to be converted from one compliance period ...
Breaking Biology News(10 mins):Short circuit in the food web 2New plant species from the heart of Texas 2New plant species from the heart of Texas 3New plant species from the heart of Texas 4New plant species from the heart of Texas 5Three reforms to protect California's cap-and-trade policy 2
... human blood group that has remained a mystery for the past ... is responsible for the lack of this blood group in some ... blood group, medical scientists can now develop a more reliable DNA ... the risk of severe, and sometimes life threatening, destruction of the ...
... Florida State University College of Medicine has made discoveries ... how life first emerged on Earth billions of years ... supporting the idea that 10 amino acids believed to ... capable of forming foldable proteins in a high-salt (halophile) ...
... water quality studies to genetic counseling, the acquisition of ... opportunity to accelerate biological and medical research in the ... the George I. Alden Trust and a $10,000 award ... Erie Community Foundation, the Mercyhurst Biology Department has purchased ...
Cached Biology News:Final chapter to 60-year-old blood group mystery 2Final chapter to 60-year-old blood group mystery 3Researcher offers clues on the origins of life 2Researcher offers clues on the origins of life 3Mercyhurst University's new DNA sequencer to accelerate scientific research in region 2