Navigation Links
RNAqueous-96 RNA Isolation Kit Automation Protocol


This protocol was developed for the MultiPROBE II HT Liquid Handling System with a Gripper Integration Platform. Time required: 1 hr 20 min.

For more information on the protocol, see the RNAqueous-96 Instruction Manual (pdf).

  1. Add 150 l Lysis Solution to each well.

  2. Add 150 l 64% ethanol to each well.

  3. Mix and transfer to the 96-well filter plate.

  4. Apply vacuum suction (20 mm Hg) for 3 min (RNA remains bound to the filter).

  5. Add 400 l Wash Solution A to each well and apply vacuum suction (20 mm Hg) for 2 min.

  6. Add 40 l (100 units) DNase I.

  7. Incubate 20 min at room temperature.

  8. Add 400 l Wash Solution B to each well and apply vacuum suction (20 mm Hg) for 2 min.

  9. Add 400 l Wash Solution C/D to each well and apply vacuum suction (20 mm Hg) for 2 min.

  10. Add 400 l 100% ethanol to each well and apply vacuum suction (20 mm Hg) for 2 min.

  11. Move 96-well filter plate to dry heat block covered with fresh paper towels and heat at 42C for 3 min (this removes any residual wash solution).

  12. Move Spacer and Collection plates to the vacuum manifold. Place 96-well filter plate on top.

  13. Add 80 l Elution Buffer to each well.

  14. Apply vacuum suction at 10 mm Hg for 30 sec then at 20 mm Hg for 2 min (RNA is transferred to collection plate).

  15. (optional) Manually check for fluid remaining on filter plate. If fluid is present, centrifuge the filter plate/collection assembly to recover residual sample.


Related Articles
RNAqueous-96 Kit Instruction Manual

High Throughput RNA Purification with Ambion RNAqueous Kits on the Robbins Tango Liquid Handling System (PDF, 136 k)


back to top


Ordering Information
Cat# Product Name Size 1920 RNAqueous-96 Kit 192 purifications
'"/>

Source:


Page: All 1 2

Related biology technology :

1. Fast and Easy Isolation of PCR-Ready Genomic DNA from Whole Blood
2. High-Throughput Isolation of Total RNA
3. Simple Isolation of RNA from Tissue and Cultured Cells
4. Fast Isolation of Total RNA from Small Numbers of Cells
5. Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit
6. Isolation of Microorganisms
7. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
8. Mouse Tail Genomic DNA Isolation Protocol(1)
9. Genomic DNA Isolation Protocol(1,2,3)
10. Basic Plasmid DNA Isolation Protocol
11. Protocol for RNA Isolation Using TRIzol Reagent with Phase Lock Gel-Heavy
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:3/22/2017)... ... March 21, 2017 , ... Okyanos Cell Therapy has ... part of their live events series, “Stem Cell Therapy: The Next Phase in the ... the 2013 Stem Cell Research and Therapy Act, Okyanos maintains a mission ...
(Date:3/22/2017)... NY (PRWEB) , ... March 21, 2017 , ... The ... Summit (CMO Summit) to be held on May 10-11, 2017, at the Colonnade ... the country specifically for Chief Medical Officer peer-to-peer learning, benchmarking and support. , “The ...
(Date:3/22/2017)... ... 22, 2017 , ... March 22, 2017...Council for Agricultural Science and Technology, Ames, ... utilizes technological innovation in smart, sustainable ways. Humans depend on plants for food, feed, ... environmental stability. This paper is the first in a series that connects science and ...
(Date:3/22/2017)... , March 22, 2017   Invitae Corporation ... genetic information companies, today announced the availability of ... Spinal Muscular Atrophy (SMA) , a neuromuscular ... genetic disorders among infants as well as a ... The new test, announced during the American College ...
Breaking Biology Technology:
(Date:3/22/2017)... 21, 2017 Vigilant Solutions , a ... enforcement agencies, announced today the appointment of retired FBI ... public safety business development. Mr. Sheridan brings ... including a focus on the aviation transportation sector, to ... position, Mr. Sheridan served as the Aviation Liaison Agent ...
(Date:3/13/2017)... Future of security: Biometric Face Matching software  ... ... Matching enables to match face pictures against each other or against large databases. ... Systems) ... the fastest software for biometric Face Matching on the market. The speed is ...
(Date:3/7/2017)... , March 7, 2017   HireVue , ... top global companies identify the best talent, faster, today ... Chief Sales Officer (CSO) and Diana Kucer ... round out a seasoned executive team poised to drive continued ... building on a year of record bookings in 2017. ...
Breaking Biology News(10 mins):