Navigation Links
RNAqueous-96 RNA Isolation Kit Automation Protocol


This protocol was developed for the MultiPROBE II HT Liquid Handling System with a Gripper Integration Platform. Time required: 1 hr 20 min.

For more information on the protocol, see the RNAqueous-96 Instruction Manual (pdf).

  1. Add 150 l Lysis Solution to each well.

  2. Add 150 l 64% ethanol to each well.

  3. Mix and transfer to the 96-well filter plate.

  4. Apply vacuum suction (20 mm Hg) for 3 min (RNA remains bound to the filter).

  5. Add 400 l Wash Solution A to each well and apply vacuum suction (20 mm Hg) for 2 min.

  6. Add 40 l (100 units) DNase I.

  7. Incubate 20 min at room temperature.

  8. Add 400 l Wash Solution B to each well and apply vacuum suction (20 mm Hg) for 2 min.

  9. Add 400 l Wash Solution C/D to each well and apply vacuum suction (20 mm Hg) for 2 min.

  10. Add 400 l 100% ethanol to each well and apply vacuum suction (20 mm Hg) for 2 min.

  11. Move 96-well filter plate to dry heat block covered with fresh paper towels and heat at 42C for 3 min (this removes any residual wash solution).

  12. Move Spacer and Collection plates to the vacuum manifold. Place 96-well filter plate on top.

  13. Add 80 l Elution Buffer to each well.

  14. Apply vacuum suction at 10 mm Hg for 30 sec then at 20 mm Hg for 2 min (RNA is transferred to collection plate).

  15. (optional) Manually check for fluid remaining on filter plate. If fluid is present, centrifuge the filter plate/collection assembly to recover residual sample.


Related Articles
RNAqueous-96 Kit Instruction Manual

High Throughput RNA Purification with Ambion RNAqueous Kits on the Robbins Tango Liquid Handling System (PDF, 136 k)


back to top


Ordering Information
Cat# Product Name Size 1920 RNAqueous-96 Kit 192 purifications
'"/>

Source:


Page: All 1 2

Related biology technology :

1. Fast and Easy Isolation of PCR-Ready Genomic DNA from Whole Blood
2. High-Throughput Isolation of Total RNA
3. Simple Isolation of RNA from Tissue and Cultured Cells
4. Fast Isolation of Total RNA from Small Numbers of Cells
5. Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit
6. Isolation of Microorganisms
7. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
8. Mouse Tail Genomic DNA Isolation Protocol(1)
9. Genomic DNA Isolation Protocol(1,2,3)
10. Basic Plasmid DNA Isolation Protocol
11. Protocol for RNA Isolation Using TRIzol Reagent with Phase Lock Gel-Heavy
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:10/11/2017)... ... October 11, 2017 , ... Disappearing forests and increased ... of over 5.5 million people each year. Especially those living in larger cities are ... - based in one of the most pollution-affected countries globally - decided to take ...
(Date:10/11/2017)... Florida (PRWEB) , ... October 11, 2017 , ... ... Drug Administration (FDA) has granted orphan drug designation to SBT-100, its novel anti-STAT3 ... for the treatment of osteosarcoma. SBT-100 is able to cross the cell membrane ...
(Date:10/10/2017)... ... October 10, 2017 , ... ... advancing targeted antibody-drug conjugate (ADC) therapeutics, today confirmed licensing rights that give ... Liposomal Nanoparticle), a technology developed in collaboration with Children’s Hospital Los Angeles ...
(Date:10/10/2017)... ... , ... Dr. Bob Harman, founder and CEO of VetStem Biopharma, Inc. ... The event entitled “Stem Cells and Their Regenerative Powers,” was held on August ... MPVM was joined by two human doctors: Peter B. Hanson, M.D., Chief of Orthopedic ...
Breaking Biology Technology:
(Date:4/17/2017)... , April 17, 2017 NXT-ID, Inc. ... company, announces the filing of its 2016 Annual Report on Form ... Exchange Commission. ... Form 10-K is available in the Investor Relations section of the ... on the SEC,s website at http://www.sec.gov . 2016 ...
(Date:4/11/2017)... -- Research and Markets has announced the addition of ... offering. ... market to grow at a CAGR of 30.37% during the period ... has been prepared based on an in-depth market analysis with inputs ... growth prospects over the coming years. The report also includes a ...
(Date:4/5/2017)... -- The Allen Institute for Cell Science today announces the ... and dynamic digital window into the human cell. The ... of deep learning to create predictive models of cell ... growing suite of powerful tools. The Allen Cell Explorer ... available resources created and shared by the Allen Institute ...
Breaking Biology News(10 mins):