Navigation Links
RNAlater Preserves Bacterial Gene Expression Profiles for Array Analysis


RNAlater Tissue Collection: RNA Stabilization Solution is the worldwide standard for the preservation of RNA in intact animal tissues, organs, and cells. RNAlater eliminates the need to immediately process tissue samples or to freeze samples in liquid nitrogen for later processing. Here we demonstrate that RNAlater provides the same outstanding benefits when used with bacteria. It immediately stabilizes RNA expression profiles in both gram-positive and gram-negative species.


Integrity Analysis of RNA Isolated From RNAlater Stabilized Bacteria
To demonstrate the effectiveness of RNAlater at preserving RNA in intact bacteria, samples from five bacterial species, Escherichia coli, Pseudomonas aeruginosa, Campylobacter fetus, Bacillus subtilis and Staphylococcus aureus, were suspended in RNAlater and incubated at 25C or 4C for up to one week. RNA was isolated from the bacteria, and RNA integrity was evaluated by analysis on the Agilent 2100 bioanalyzer and by filter based array analysis.

Log phase Bacillus subtilis cells were harvested by centrifugation, resuspended in RNAlater (or TE as a negative control), and incubated for four hours at room temperature or one week at 4C. RNA was isolated from cells by bead-beating with zirconium/silica beads in RNAwiz (Ambion). RNA integrity was evaluated using the RNA 6000 LabChip and the Agilent 2100 bioanalyzer (Figure 1, A-D). Evaluation of the electropherogram
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Preserve Samples for RNA Expression Microarrays RNAlater Around the World
2. RNAlater FAQs: Preserving RNA Before Isolation
3. RNAlater Around the World: The Art of Processing Difficult Samples
4. Your Data: RNAlater Stories
5. RNArticles: Keeping RNA Intact Prior to RNA Isolation - RNAlater
6. Genomic DNA Preparation from RNAlater Preserved Tissues
7. High-Throughput Cultivation of Bacterial Clones
8. Mammalian and Bacterial Expression in One Vector
9. Bacterial mRNA Isolation, Fast and Easy
10. Bacterial RNA Isolation From Infected Eukaryotic Hosts
11. Bacterial RNA Isolation From Infected Eukaryotic Hosts
Post Your Comments:
(Date:5/5/2015)... DIEGO , May 5, 2015  A group ... formation of the Coma Research Institute, a non-profit organization ... patients and families suffering from disorders of consciousness.  ... - http://photos.prnewswire.com/prnh/20150504/213779LOGO   ... disorder of consciousness, in which no voluntary actions can ...
(Date:5/5/2015)... May 05, 2015 Franz Inc ., ... today announced that its flagship product, AllegroGraph, has been named ... Database Market Update report. AllegroGraph is a high ... W3C industry standards. Graph databases are skyrocketing in popularity and ... to a recent DBMS ranking by DB-Engines. , ...
(Date:5/5/2015)... --Research and Markets ( http://www.researchandmarkets.com/research/4r95kr/clinical ) ... Laboratory, Molecular Diagnostics and Genomic Testing Services Market ... Version" report to their offering. ... molecular diagnostics and pharmacogenics hold the promise of ... is moving out of the national and regional ...
(Date:5/5/2015)...  Orexigen Therapeutics, Inc. (Nasdaq: OREX ) will ... Friday, May 8 before the market opens. The announcement ... call at 8:30 a.m. Eastern Time (5:30 a.m. Pacific ... by phone by calling (800) 708-4540 (domestic) or (847) ... accessed live on the Investors section of the Orexigen ...
Breaking Biology Technology:Biotechnology Executives and Medical Doctors Team Up to Tackle Coma and Disorders of Consciousness 2Biotechnology Executives and Medical Doctors Team Up to Tackle Coma and Disorders of Consciousness 3Biotechnology Executives and Medical Doctors Team Up to Tackle Coma and Disorders of Consciousness 4Biotechnology Executives and Medical Doctors Team Up to Tackle Coma and Disorders of Consciousness 5Bloor Research Positions AllegroGraph as a ‘Champion’ in Burgeoning Graph Database Market 2Bloor Research Positions AllegroGraph as a ‘Champion’ in Burgeoning Graph Database Market 3European Clinical Laboratory, Molecular Diagnostics and Genomic Testing Services Market 2015-2019 2
... elected mayor of Colorados largest city, businessman John Hickenlooper invited ... was to ceremoniously bury the hatchet of intra-regional rivalry and ... metropolitan area, or not at all. , ,Every suburban ... a near-unanimous effort to pass a $4.7-billion mass transit initiative ...
... data quality firm Firstlogic for about $69 million ... second quarter. , ,Firstlogic is a privately held firm that ... sure their data is as correct as they need to ... call centers, web sites, customer relationship management systems, and business ...
... settled with a Belgian company that sued last September ... virus genotyping technology from Innogenetics, for undisclosed financial terms. ... Aril 2004 Third Wave forged a deal under which ... line of products in Europe. It was Third Wave's ...
Cached Biology Technology:Economic collaboration in Denver sets tall goal for Wisconsin 2Economic collaboration in Denver sets tall goal for Wisconsin 3
(Date:3/24/2015)... , Mar. 24, 2015 Research and Markets ... the "Global Iris Recognition Market 2015-2019" report ... Global Iris Recognition market to grow at a CAGR ... This report covers the present scenario and the growth ... period 2015-2019. To calculate the market size, the report ...
(Date:3/24/2015)... , March 24, 2015   NexID Biometrics ... with critical security-authentication needs, today announced the beginning of ... (FFD) solution. The company, based in ... at the three-day Connect:ID Expo, which began here today ... NexID said version 2.0 of its SDK boosts the ...
(Date:3/23/2015)... BOSTON, Mass , March 23, 2015 SoundView ... shipment of NXT-ID,s (NASDAQ: NXTD ) Wocket smart wallet. SoundView ... world user feedback on their experience with the Wocket in ... experience at CVS, Whole Foods and other retailers, making both ... Kris Tuttle also says, "If the company meets ...
Breaking Biology News(10 mins):Global Iris Recognition Market 2015-2019 with 3M Cogent, Bioenable Technologies, Cross Match Technology, Iris ID Systems & Safran Dominating 2NexID Begins Shipments Of Version 2.0 Fake-Finger-Detection Solution 2Technology Research Note Update from SoundView; Real-World Usage and Feedback of Wocket Smart Wallet at CVS, Whole Foods and other Retailers 2Technology Research Note Update from SoundView; Real-World Usage and Feedback of Wocket Smart Wallet at CVS, Whole Foods and other Retailers 3Technology Research Note Update from SoundView; Real-World Usage and Feedback of Wocket Smart Wallet at CVS, Whole Foods and other Retailers 4
... 2013 The report "Maritime Security Market ... and Tracking (Underwater, Video, AIS, LRIT, Imaging); Detectors; SCADA; ... - 2018)", defines and segments the global Maritime Security ... forecast of revenues. It also identifies drivers and restraints ...
... , November 26, 2013 L,appareil et ... extérieur dans plus de 30 pays Wonder Technology ... vient de lancer Trax, un traceur GPS pour enfants ... et son application permettent aux utilisateurs de localiser leurs ...
... sunlight. While some wavelengths are indispensable to them for ... plants are equipped to detect these highly toxic rays ... Ulm, Professor at the Faculty of Sciences at the ... plant which acclimatises constitutively, regardless of the level of ...
Cached Biology News:Maritime Security Market Worth $19.48 Billion by 2018 2Maritime Security Market Worth $19.48 Billion by 2018 3Suivez vos enfants et vos animaux domestiques grâce à TRAX, le nouveau traceur GPS intelligent 2Suivez vos enfants et vos animaux domestiques grâce à TRAX, le nouveau traceur GPS intelligent 3A plant which acclimatizes with no exterior influence 2