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RNAlater Preserves Bacterial Gene Expression Profiles for Array Analysis


RNAlater Tissue Collection: RNA Stabilization Solution is the worldwide standard for the preservation of RNA in intact animal tissues, organs, and cells. RNAlater eliminates the need to immediately process tissue samples or to freeze samples in liquid nitrogen for later processing. Here we demonstrate that RNAlater provides the same outstanding benefits when used with bacteria. It immediately stabilizes RNA expression profiles in both gram-positive and gram-negative species.


Integrity Analysis of RNA Isolated From RNAlater Stabilized Bacteria
To demonstrate the effectiveness of RNAlater at preserving RNA in intact bacteria, samples from five bacterial species, Escherichia coli, Pseudomonas aeruginosa, Campylobacter fetus, Bacillus subtilis and Staphylococcus aureus, were suspended in RNAlater and incubated at 25C or 4C for up to one week. RNA was isolated from the bacteria, and RNA integrity was evaluated by analysis on the Agilent 2100 bioanalyzer and by filter based array analysis.

Log phase Bacillus subtilis cells were harvested by centrifugation, resuspended in RNAlater (or TE as a negative control), and incubated for four hours at room temperature or one week at 4C. RNA was isolated from cells by bead-beating with zirconium/silica beads in RNAwiz (Ambion). RNA integrity was evaluated using the RNA 6000 LabChip and the Agilent 2100 bioanalyzer (Figure 1, A-D). Evaluation of the electropherogram
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