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RNAlater FAQs: Preserving RNA Before Isolation

extract both genomic DNA and total protein from samples stored in RNAlater. RNAlater will denature proteins, so it is only compatible with routine protein analyses such as Western blotting and 2D gel electrophoresis that do not require native protein [protocols] .

Note: RNAlater is not recommended for use with tissues that will be sectioned using a cryostat.


5) How can I ship my sample in RNAlater?
Samples in RNAlater can safely be shipped on wet ice for several days. For longer shipping times use dry ice.


6) Can I add RNAlater to samples that are already frozen?
A different product, RNAlater-ICE, is used with samples that are already frozen. RNAlater-ICE transitions tissue from a frozen to a non-frozen state. The frozen tissue is simply placed in RNAlater-ICE and left at -20C overnight. Treated tissues can then be used directly in standard homogenization and isolation protocols and processed like fresh tissue.

RNAlater and RNAlater-ICE provide flexibility for sample collection and storage, and help ensure that high quality RNA is preserved in samples. Both are available in a variety of convenient sizes.


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