( ater are protected from RNases as long...)RNAlater FAQs: Preserving RNA Before Isolation

ater are protected from RNases as long as the tissue remains in the solution, and they can typically be disrupted using the simpler methods appropriate for freshly collected samples (grinding in liquid nitrogen is only required for extremely hard or tough tissues such as bone or tumor tissue). Thus, in addition to making sample disruption easier, storage in RNAlater eliminates the risks of sample loss and mess due to transferring or thawing frozen powdered tissue.

3) Has RNAlater been tested with my tissue or with blood samples?
RNAlater has been successfully tested with many different tissues, (including brain, heart, kidney, liver, skeletal muscle, fat, lung) and cell types (E. coli, Drosophila, cultured mammalian cells, and some plant cells). RNAlater can also be used to store anticoagulated whole blood or the white blood cell fraction of whole blood. Ambion's RiboPure-Blood RNA Isolation Kit incorporates RNAlater and provides instructions on how best to use RNAlater with blood.

4) Which downstream applications can be used with tissue stored in RNAlater?
Tissue stored in RNAlater is compatible with all commonly used RNA isolation methods, including single reagent isolation products like Trizol, and all of Ambion's RNA isolation kits. It is also possible to
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