Navigation Links
RNAlater FAQs: Preserving RNA Before Isolation


Appropriate storage of cells or tissue samples prior to RNA isolation is critical for preservation of intact RNA within those samples. RNAlater is an aqueous solution designed specifically to maintain RNA integrity during storage of fresh tissue and cell samples. Here are some commonly asked questions regarding RNAlater:


1) How do I use RNAlater to store my tissue/cell sample?
For fresh tissue, simply cut samples to a maximum thickness of 0.5 cm in any one dimension and submerge in 5 volumes of RNAlater.

Cultured cells should be pelleted, resuspended in a small volume of PBS, then mixed with 5-10 volumes of RNAlater.

Once in RNAlater, samples can be stored for up to 1 day at 37C, 1 week at 25C, 1 month or more at 4C, and long-term at -20C or -80 .


2) Does storing samples in RNAlater have advantages over freezing samples on dry ice or in liquid nitrogen?
Using samples that were stored in RNAlater is much easier than using frozen samples. Frozen samples must be ground to a powder and then the frozen powder must be transferred to a tube for homogenization. This procedure is laborious, messy, risks loss of sample, and perhaps most importantly, may lead to sample thawing, which can compromise RNA integrity. Samples stored in RNAl
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Preserve Samples for RNA Expression Microarrays RNAlater Around the World
2. RNAlater Preserves Bacterial Gene Expression Profiles for Array Analysis
3. RNAlater Around the World: The Art of Processing Difficult Samples
4. Your Data: RNAlater Stories
5. RNArticles: Keeping RNA Intact Prior to RNA Isolation - RNAlater
6. Genomic DNA Preparation from RNAlater Preserved Tissues
7. Fast and Easy Isolation of PCR-Ready Genomic DNA from Whole Blood
8. High-Throughput Isolation of Total RNA
9. Simple Isolation of RNA from Tissue and Cultured Cells
10. Fast Isolation of Total RNA from Small Numbers of Cells
11. Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: RNAlater FAQs Preserving RNA Before Isolation

(Date:12/24/2014)... PharmaBoardroom,s new report, , ... available for free download , digs deep into this ... in the sector today. One area where ... in developing a homegrown pharmaceutical manufacturing base, even if the ... some way off. A cursory comparison with neighboring ...
(Date:12/24/2014)... and NEW YORK , ... from its open-label pilot study of mazindol in children ... Development and Therapy in December 2014 . ... mazindol in children with attention deficit/hyperactivity disorder" ( Konofal ... 2014 Dec 1;8:2321-2332. eCollection 2014 ) shows ...
(Date:12/22/2014)... - RepliCel Life Sciences Inc. (OTCQB: REPCF) (TSX.V: RP), ... development of autologous cell therapies, announced today the publication ... in conjunction with co-authors from Kyoto University and the ... ongoing clinical research using dermal sheath cup (DSC) cells ... The paper entitled " Hair Follicle Dermal Stem Cells ...
(Date:12/22/2014)... , Dec. 22, 2014  ( www.competitivehealth.com ) ... an online medical bill review and advocacy service, has ... the FREE WellCard Savings discount health services marketplace. ... that was more than they expected to pay. As ... health care costs, WellCard Savings is pleased to offer ...
Breaking Biology Technology:PharmaBoardroom Releases New Algeria Pharmaceuticals Report 2PharmaBoardroom Releases New Algeria Pharmaceuticals Report 3Redefining ADHD: A New Approach & A Shift of Paradigm in ADHD Therapeutics 2Redefining ADHD: A New Approach & A Shift of Paradigm in ADHD Therapeutics 3Newly Released Peer-Reviewed Publication Further Validates RepliCel's Use of Dermal Sheath Cup Cells to Treat Pattern Baldness 2Newly Released Peer-Reviewed Publication Further Validates RepliCel's Use of Dermal Sheath Cup Cells to Treat Pattern Baldness 3Newly Released Peer-Reviewed Publication Further Validates RepliCel's Use of Dermal Sheath Cup Cells to Treat Pattern Baldness 4Newly Released Peer-Reviewed Publication Further Validates RepliCel's Use of Dermal Sheath Cup Cells to Treat Pattern Baldness 5WellCard Savings Announces CoPatient Medical Bill Review Service for Members 2
... Positive results from Pyratine XR(TM) rosacea-specific study also slated ... March 8, 2009 - NAPA, Calif., March 5 ... sciences company engaged in the development of technologies that ... Pyratine XR(TM) is officially launching at the American Academy ...
... the launch of a suite of miRNA functional ... vector based precursor miRNA expression clones; 2) miTarget(TM) ... 3) All-in-One(TM) miRNA RT-qPCR system.Constructed in a lentiviral ... collections cover all known miRNAs for human ...
... 5 Poniard Pharmaceuticals, Inc. (Nasdaq: ... oncology therapies, today announced that it will hold ... quarter and year-end financial results and provide a ... the Company,s lead product candidate.Jerry McMahon, Ph.D., chairman ...
Cached Biology Technology:Pyratine XR(TM) Launches at 67th AAD Meeting, Offering Hope to 14 Million Rosacea Sufferers 2Pyratine XR(TM) Launches at 67th AAD Meeting, Offering Hope to 14 Million Rosacea Sufferers 3GeneCopoeia Introduces Genome-Wide MicroRNA (miRNA) Functional Analysis Tools 2Poniard Pharmaceuticals to Host Fourth Quarter and Year-End 2008 Financial Results Conference Call on March 12 2
(Date:12/5/2014)... VIEW, Calif. , Dec. 4, 2014 /PRNewswire/ ... safety has boosted investments in new testing and ... has also given rise to a range of ... by the requirements of Generation Y, which is ... technologies. As a result, product development strategies of ...
(Date:12/4/2014)... 3, 2014  Crossmatch™, a leading provider of ... DigitalPersona ® Pro Enterprise software and U.are.U ... throughout First Bank branch locations in ... and Virginia. First Bank, a progressive community ... North Carolina , selected the Crossmatch biometrics-based ...
(Date:12/3/2014)... , Dec. 2, 2014 As part ... readers, Inception Technologies is pleased to announce the release ... for customers to collect the workforce data that they ... that have been left by existing readers. Many such ... interface, connectivity and modern technology. Older models force users ...
Breaking Biology News(10 mins):Emerging Technologies Enable Smarter Monitoring of Assets, Push Industrial Testing, Inspection and Monitoring Market 2Emerging Technologies Enable Smarter Monitoring of Assets, Push Industrial Testing, Inspection and Monitoring Market 3Emerging Technologies Enable Smarter Monitoring of Assets, Push Industrial Testing, Inspection and Monitoring Market 4First Bank Partners with Crossmatch to Increase Log In Security 2First Bank Partners with Crossmatch to Increase Log In Security 3Inception Technologies to Release New Biometric Reader 2
... International Liver CongressTM 2013 has demonstrated the, accuracy of ... Non Alcoholic Fatty Liver disease (NAFLD) comprises two ... relatively benign and one group with NASH which may ... the only means of distinguishing the two was to ...
... 23, 2013  Aware, Inc. (NASDAQ: AWRE ), a ... assurance software products, today reported financial results for its first ... the first quarter of 2013 was $5.6 million, an increase ... last year. Operating income before patent related income in the ...
... April 23, 2013 Governor Tom Corbett ... with its 2013 BIO Governor of the Year Award in ... and public health sectors within Pennsylvania . ... BIO International Convention in Chicago, Ill. ...
Cached Biology News:Aware, Inc. Reports First Quarter 2013 Financial Results 2Aware, Inc. Reports First Quarter 2013 Financial Results 3Aware, Inc. Reports First Quarter 2013 Financial Results 4Governor Corbett Presented 2013 Biotechnology Industry Organization Governor of the Year Award 2Governor Corbett Presented 2013 Biotechnology Industry Organization Governor of the Year Award 3
... Mutation Explorer processes up to 48 ... traces. Data input can be from either ... terminator or primer chemistries. The detection parameters ... analyst to analyst consistency. We have ...
Molecular Biological Resources offers bulk quantities of existing protein products. Please see the Chimerx divisions website for existing products and contact lloconne@molbiores.com for pricing....
... Certified USDA Research Registered OLAW Assured AAALAC ... AALAS Certified Technicians Documentation ... Upon Request Focus CRP is ... your custom immunology service needs. We ...
... XL provides balance data collection and automation that ... integration with Microsoft Excel , Pre-configured library ... , New balances can be added and configured ... from multiple balances into one spreadsheet ...
Biology Products: