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RNAlater Around the World: The Art of Processing Difficult Samples

pathways or to RNA degradation were reduced. To learn more, see Genes Immun (2004) 5: 347-353.


Environmental Bacteria--Netherlands Antilles

To study differential gene expression in bacterial communities associated with coral black band disease (BBD), scuba-diving scientists collected active BBD mat samples from infected coral surfaces and BBD mat samples that had been moved 0.3 to 1 m from the reef. Upon removal from the ocean, their samples were stabilized in RNAlater for eventual RNA-arbitrarily primed PCR (RAP-PCR) and RT-PCR analyses. These experiments are providing important clues to the global problem of BBD migration as well as the infection and destruction of coral reefs by BBD. To learn more, see Appl Environ Microbiol (2004) 70: 3687-94.


Environmental Virus-- Laos People's Democratic Republic

To find ways of improving diagnostic testing of classical swine fever (hog cholera), researchers simulated collection and transport conditions in tropical ambient temperatures for virus-positive spleen samples in RNAlater or a glycerol/saline buffer. In both widely used diagnostic tests (RT-PCR and antigen-capture ELISA), viral detection was more sensitive in samples preserved with RNAlater. To learn more, see J Virol Methods (2004) 118: 33-7.


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