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RNAlater Around the World: The Art of Processing Difficult Samples


Draw on the strength of RNAlater to protect cellular RNA within specimens during sample collection or shipping. Whether you are in the lab or out in the field, this aqueous, non-toxic Tissue Collection:RNA Stabilization Solution functions from ambient temperatures down to -20C. Here is a snapshot of how researchers have used RNAlater in exciting discoveries around the world.


Pancreas--Germany

Isolating intact RNA from pancreas can be difficult because of high levels of nucleases expressed in this tissue. In a study about circadian expression of clock genes, scientists used RNAlater to collect rat pancreata at three hour intervals for eventual quantitative RT-PCR analyses. These experiments provide growing evidence of circadian pacemakers in islets and begin to examine the role of dysrhythmic insulin secretion at the onset of diabetes mellitus. To learn more, see FEBS Lett (2004) 564: 91-96.


Blood--United States

RNAlater can be used to protect RNA in blood samples for any research or diagnostic test. One research group has shown that this may be critical for maintaining gene expression patterns in peripheral blood mononuclear cells (PBMCs). When PBMCs were resuspended in RNAlater within 30 minutes of collection, major changes in gene expression profiles related to ex vivo stress response
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