Navigation Links
RNAlater Around the World: The Art of Processing Difficult Samples


Draw on the strength of RNAlater to protect cellular RNA within specimens during sample collection or shipping. Whether you are in the lab or out in the field, this aqueous, non-toxic Tissue Collection:RNA Stabilization Solution functions from ambient temperatures down to -20C. Here is a snapshot of how researchers have used RNAlater in exciting discoveries around the world.


Pancreas--Germany

Isolating intact RNA from pancreas can be difficult because of high levels of nucleases expressed in this tissue. In a study about circadian expression of clock genes, scientists used RNAlater to collect rat pancreata at three hour intervals for eventual quantitative RT-PCR analyses. These experiments provide growing evidence of circadian pacemakers in islets and begin to examine the role of dysrhythmic insulin secretion at the onset of diabetes mellitus. To learn more, see FEBS Lett (2004) 564: 91-96.


Blood--United States

RNAlater can be used to protect RNA in blood samples for any research or diagnostic test. One research group has shown that this may be critical for maintaining gene expression patterns in peripheral blood mononuclear cells (PBMCs). When PBMCs were resuspended in RNAlater within 30 minutes of collection, major changes in gene expression profiles related to ex vivo stress response pathways or to RNA degradation were reduced. To learn more, see Genes Immun (2004) 5: 347-353.


Environmental Bacteria--Netherlands Antilles

To study differential gene expression in bacterial communities associated with coral black band disease (BBD), scuba-diving scientists collected active BBD mat samples from infected coral surfaces and BBD mat samples that had been moved 0.3 to 1 m from the reef. Upon removal from the ocean, their samples were stabilized in RNAlater for eventual RNA-arbitrarily primed PCR (RAP-PCR) and RT-PCR analyses. These experiments are providing important clues to the global problem of BBD migration as well as the infection and destruction of coral reefs by BBD. To learn more, see Appl Environ Microbiol (2004) 70: 3687-94.


Environmental Virus-- Laos People's Democratic Republic

To find ways of improving diagnostic testing of classical swine fever (hog cholera), researchers simulated collection and transport conditions in tropical ambient temperatures for virus-positive spleen samples in RNAlater or a glycerol/saline buffer. In both widely used diagnostic tests (RT-PCR and antigen-capture ELISA), viral detection was more sensitive in samples preserved with RNAlater. To learn more, see J Virol Methods (2004) 118: 33-7.


back to top


Ordering Information
Cat# Product Name Size 7020 RNAlater 100 ml 7021 RNAlater 500 ml 7022 RNAlater 50 x 1.5 ml 7023 RNAlater 20 x 5 ml 7024 RNAlater 250 ml
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Preserve Samples for RNA Expression Microarrays RNAlater Around the World
2. RNAlater Preserves Bacterial Gene Expression Profiles for Array Analysis
3. RNAlater FAQs: Preserving RNA Before Isolation
4. Your Data: RNAlater Stories
5. RNArticles: Keeping RNA Intact Prior to RNA Isolation - RNAlater
6. Genomic DNA Preparation from RNAlater Preserved Tissues
7. New Tools for Making and Processing Protein Microarrays.
8. Amplification of Epstein-Barr Virus Exon C with Difficult Primers Using Eppendorf Mastercycler gradient
9. Delivering siRNAs to Difficult Cell Types
10. Quantitation of the Protein MIA as a Marker for Chondrocytes in Research Samples
11. Isolate High Quality Total RNA from LCM Samples Suitable for Microarray and qRT-PCR Analysis
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:3/28/2017)... 2017  The National Pharmaceutical Council (NPC) today announced ... research organization as its newest member. David Cox ... North America ), will serve as his ... "We,re pleased to have Ipsen and Dr. Cox join ... . "We welcome their insights in helping us identify ...
(Date:3/28/2017)... 2017 Biostage, Inc. (Nasdaq: BSTG), ... organ implants to treat cancers and other life-threatening conditions ... Jim McGorry, CEO of Biostage, will present ... at the MassBio 2017 Annual Meeting on ... Cambrige, Massachussetts. The 3D Printing and BioEngineering ...
(Date:3/28/2017)... 2017 Focus is on ... due to the scientifically intensive operations of companies ... environmental, and industrial. In today,s pre-market research, Stock-Callers.com dedicates ... (NASDAQ: PTLA), OvaScience Inc. (NASDAQ: OVAS), Ocera Therapeutics ... OCUL ). Learn more about these ...
(Date:3/28/2017)... , ... March 28, 2017 , ... ... the development of a new orally administered treatment for Alzheimer’s disease (AD), today ... John Didsbury states, “As we seek to uniquely treat the metabolic dysfunctions inherent ...
Breaking Biology Technology:
(Date:3/13/2017)... , March 13, 2017 Future of security: Biometric Face ... ... DERMALOGs Face Matching enables to match face pictures against ... basis to identify individuals. (PRNewsFoto/Dermalog Identification Systems) ... DERMALOG,s "Face Matching" is the fastest software for biometric Face Matching ...
(Date:3/6/2017)... Calif. , March 6, 2017 ... and sales technology, today announced Predictive Sales Coach ... for infusing actionable sales intelligence into Salesforce. This ... automatically enable their sales organizations with deep knowledge ... that allow for intelligent engagement. Predictive Sales Coach ...
(Date:3/1/2017)... Mass. , March 1, 2017  Aware, Inc. ... services, announced that Richard P. Moberg has ... and co-President and Chief Financial Officer and Treasurer of ... continue to serve as a member of the Board ... Russell , Aware,s co-Chief Executive Officer and co-President, General ...
Breaking Biology News(10 mins):