RNAi in Non-Mammalian Cultured Cells ( siRNA Labeling Kits wer...)

RNAi in Non-Mammalian Cultured Cells

siRNA Labeling Kits were developed for labeling siRNA, they can be readily adapted for labeling long dsRNA. A protocol for this application can be found at www.ambion.com/techlib/misc/labeling_dsRNA.html

REFERENCES

1. Brown D, Jarvis R, Pallotta V, Byrom M, and Ford L. (2002) RNA Interference in Mammalian Cell Culture: Design, Execution and Analysis of the siRNA Effect. Ambion TechNotes 9(1):35.

2. Jarvis R and Ford L (2001) The siRNA Target Site is an Important Parameter for Inducing RNAi in Human Cells. Ambion TechNotes 8(5):35.

3. Byron M, Pallotta V, Brown B, and Ford L. (2002) Visualizing siRNA in Mammalian Cells: Fluorescence Analysis of the RNAi Effect. Ambion TechNotes 9(3):68.

Cy3 is a trademark of Amersham Biosciences.

The Cy3 and Fluorescein Labeling reagents manufactured for Ambion by Mirus Corporation.

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( siRNA Labeling Kits wer...)RNAi in Non-Mammalian Cultured Cells