Navigation Links
RNAi for High Throughput Analysis


Large scale gene expression studies require reliable high-throughput methods to synthesize and then purify high quality RNA from a large number of samples. Although the MEGAscript RNAi Kit includes reagents for both dsRNA production and clean up, it is not optimized for clean up in a 96 well plate format. Thus for high-throughput applications, we recommend combining Ambion's MEGAscript High Yield Transcription Kit with MEGAclear-96, which is designed specifically for high-throughput and rapid clean up of large scale transcription reactions.

To demonstrate the utility of this approach, Ambion developed an automation protocol based on the MEGAclear technology to purify RNA from high yield in vitro transcription reactions. Two dsRNAs specific for the U2AF50 gene product, synthesized using MEGAscript, were purified using the MEGAclear-96 Kit on the Packard MultiPROBE II HT or the Biomek 2000 platforms. The activities of the purified dsRNAs were then tested in Drosophila L2 cells. As shown in Figure 1, a silencing effect similar to dsRNAs prepared and purified with the MEGAscript RNAi Kit was observed.

The MEGAclear-96 Kit can clean up as many as 96 samples in just half an hour. This procedure can be integrated with your favorite robotic platform or used with manual methods for many downstream applications, including RNA interference, aRNA amplification, array hybridization and translation studies.

Figure 1. Comparison of dsRNA Prepared and Purified with the MEGAscript RNAi Kit vs. the MEGAscript Kit Followed by MEGAclear-96 . Western blot analysis was performed on Drosophila L2 cells 72 hr post treatment with 1 or 10 nM of the indicated U2AF50-specific dsRNA. RNAi Kit: dsRNA prepared and purified using the MEGAscript RNAi Kit. Mega 96: dsRNA prepared with the MEGAscript Kit (Cat #1334) and purified with MEGAclear-96 (Cat. #1909).


back to top


Ordering Information
For prices and availability, please contact our Customer Service Department.
Cat# Product Name Size 1334 MEGAscript T7 Kit 40 rxns 1626 MEGAscript RNAi Kit 20 rxns 1909 MEGAclear-96 Kit 96 rxns
'"/>

Source:


Page: All 1 2

Related biology technology :

1. Innovative Tissue Array Technology for High-Throughput Screening of Gene Expression
2. Highest Possible Transformation Efficiencies for High-Throughput Applications
3. High-Throughput Cultivation of Bacterial Clones
4. High-Throughput Isolation of Total RNA
5. High-Throughput System Generates Ultra-Pure PCR Products Suitable for All Applications
6. The DIG System Nonradioactive Automated High-Throughput In Situ Hybridization: a Powerful Tool for Functional Genomics Research
7. High Throughput aRNA Amplification
8. High Throughput siRNA Electroporation
9. High Throughput siRNA Delivery In Vitro: From Cell Lines to Primary Cells
10. High Throughput RNA Isolation for High Performance Microarray Analysis
11. Robust High Throughput RNA Isolation from Blood Samples
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:10/12/2017)... ... October 12, 2017 , ... The Blavatnik Family ... and six Finalists of the 2017 Blavatnik Regional Awards for Young Scientists. ... and administered by the New York Academy of Sciences to honor the excellence ...
(Date:10/12/2017)... ... 2017 , ... AMRI, a global contract research, development and ... outcomes and quality of life, will now be offering its impurity solutions as ... regulatory requirements for all new drug products, including the finalization of ICH M7 ...
(Date:10/11/2017)... ... 11, 2017 , ... Personal eye wash is a basic first aid supply for any work ... which eye do you rinse first if a dangerous substance enters both eyes? It’s one ... with its unique dual eye piece. , “Whether its dirt and debris, or an ...
(Date:10/11/2017)... and LAGUNA HILLS, Calif. , Oct. ... Cancer Research, London (ICR) and University ... SKY92, SkylineDx,s prognostic tool to risk-stratify patients with multiple myeloma ... MUK nine . The University of Leeds ... partly funded by Myeloma UK, and ICR will perform the ...
Breaking Biology Technology:
(Date:6/23/2017)... ITHACA, N.Y. , June 23, 2017 /PRNewswire/ ... leader in dairy research, today announced a new collaboration ... reduce the chances that the global milk supply is ... dairy project, Cornell University has become the newest academic ... Supply Chain, a food safety initiative that includes IBM ...
(Date:5/6/2017)... 5, 2017 RAM Group , ... new breakthrough in biometric authentication based on a ... properties to perform biometric authentication. These new sensors are ... created by Ram Group and its partners. This sensor ... supply chains and security. Ram Group is a ...
(Date:4/13/2017)... SANTA MONICA, Calif. , April 13, 2017 /PRNewswire/ ... New York will feature emerging and ... Innovation Summits. Both Innovation Summits will run alongside the ... variety of speaker sessions, panels and demonstrations focused on ... east coast,s largest advanced design and manufacturing event will ...
Breaking Biology News(10 mins):