( tegorized based on their response. The ...)RNAi as a Tool for Mammalian Gene Analysis: Applications of siRNAs

tegorized based on their response. The genes that were highly and quickly up-regulated included the proto-oncogene, fra-1. siRNA-induced reduction in fra-1 expression caused an increase in the expression of both cd44 and c-met, connecting fra-1 with genes governing cell motility and invasion in mesothelioma.

Gene Redundancy
In many cases, eliminating the expression of a single gene in higher eukaryotes can be tolerated even if that gene product functions in a critical pathway. This is because many critical cell functions are accomplished by more than one gene product. When one gene product is eliminated, the redundant gene product compensates to allow the cell or animal to survive. Identifying redundant genes could be achieved by co-transfecting siRNAs and assaying for a given phenotype. For example, a gene that is identified as being important in cell cycle regulation might fail to elicit a cell-cycle defective phenotype. Co-transfecting this siRNA with other siRNAs targeting other cell cycle genes and assaying for a cell cycle phenotype could identify genes that might serve at the same point in the cell cycle. Evaluating each of the candidate genes alone to ensure that they only cause the cell cycle defect when reduced in combination with the target gene would help pinpoint the most likely redundant gene.

* Glucose levels are thought to be regulated by the family of Akt serine/threonine kinases. When Katome et al (8) reduced
'"/>

Source:

Page: All 1 2 3 4 5 6 7 8
Related biology technology :

1. New Mammalian Two-Hybrid System Detects Protein-Protein Interactions
2. Highest Transfection Efficiency of an Endotoxin-Sensitive Mammalian Cell Line
3. An Epitope Tagging Vector for Gene Expression in Mammalian Cells
4. Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
5. New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter
6. A New Lambda Vector for Mammalian Expression
7. Mammalian and Bacterial Expression in One Vector
8. Mammalian Expression Vector for Efficient Cloning of PCR Fragments
9. Mammalian Expression Vectors with Interchangeable Selectable Markers
10. Low-Toxicity, Lipid-Mediated Transfection of Mammalian Cells
11. Mycoplasma Contamination Reduces the Effect of Lipid-Mediated Transfection of Mammalian Cells