Navigation Links
RNAi as a Tool for Mammalian Gene Analysis: Applications of siRNAs

David Brown (Senior Scientist, Ambion, Inc.)


The realization that RNA interference (RNAi) can be used as a general tool for mammalian gene function studies occurred with the seminal Elbashir et al. (1) publication describing the use of small interfering RNAs (siRNAs) in mammalian cells in 2001. In less than two years, siRNAs have become the cornerstone of many research programs. This rapid uptake has resulted primarily from the ease of use of siRNAs and the strong need for a method to reduce the expression of individual genes in mammalian cells in order to establish a link between gene identity and gene function. Provided below are some of the more prominent applications for RNAi in mammalian systems and examples of these applications from the literature.


Testing Hypotheses of Gene Function
Array analysis and other methods for identifying differentially expressed genes have created an enormous database of genes and associated phenotypes. In many cases, scientists make predictions about gene function based on expression patterns in different samples. Other predictions of mammalian gene function are developed using homology searches with genes whose functions are known in model organisms like Drosophila, C. elegans, and S. cerevisiae. In many cases, testing the accuracy of these predictions can be accomplished using siRNAs. <
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. New Mammalian Two-Hybrid System Detects Protein-Protein Interactions
2. Highest Transfection Efficiency of an Endotoxin-Sensitive Mammalian Cell Line
3. An Epitope Tagging Vector for Gene Expression in Mammalian Cells
4. Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
5. New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter
6. A New Lambda Vector for Mammalian Expression
7. Mammalian and Bacterial Expression in One Vector
8. Mammalian Expression Vector for Efficient Cloning of PCR Fragments
9. Mammalian Expression Vectors with Interchangeable Selectable Markers
10. Low-Toxicity, Lipid-Mediated Transfection of Mammalian Cells
11. Mycoplasma Contamination Reduces the Effect of Lipid-Mediated Transfection of Mammalian Cells

Post Your Comments:
*Name:
*Comment:
*Email:
TAG: RNAi Tool for Mammalian Gene Analysis Applications siRNAs

(Date:11/5/2009)...USTIN, Texas, Nov. 5 SpineSmith Pa...eo, Inc. to co-develop a unique scaffold that mimi...owing evaluation of several commercial products by... the company believes the GenOsteo scaffold repres...creates a unique and ideal environment for the del...
(Date:11/5/2009)... LASIK Surgery and vision correc...directory were actively involved at the recently c...009 Annual Meeting in San Francisco, California. D...D, Dr. Jonathan Davidorf, MD, Dr. Peter Rapoza, MD... Dr. R. Wayne Bowman, MD, Dr. Edward Manche, MD, a...
(Date:11/4/2009)... The Human Anthrax Vaccine Progr...completed the feature film "A Call to Arms:20...entary "Fatal Immunity: The Human Anthrax Vac...ear and present danger of top secret government sc...s with no accountability to its victims. It is av...
(Date:11/4/2009)... ,, ROCKVILLE, Md., Nov. 4 Zy...pany focused on next-generation antibody drug deve...illion in Series A funding from New Enterprise Ass...capital firms. The capital will be used to create ... address two or more biological targets with a sin...
Breaking Biology Technology:SpineSmith Partners and GenOsteo Announce Collaborative Product Development Agreement 2Leading Vision Correction Experts in Trusted LASIK Surgeons Directory Educate Fellow Doctors at AAO Annual Meeting 2Leading Vision Correction Experts in Trusted LASIK Surgeons Directory Educate Fellow Doctors at AAO Annual Meeting 3Leading Vision Correction Experts in Trusted LASIK Surgeons Directory Educate Fellow Doctors at AAO Annual Meeting 4Leading Vision Correction Experts in Trusted LASIK Surgeons Directory Educate Fellow Doctors at AAO Annual Meeting 5Leading Vision Correction Experts in Trusted LASIK Surgeons Directory Educate Fellow Doctors at AAO Annual Meeting 6Leading Vision Correction Experts in Trusted LASIK Surgeons Directory Educate Fellow Doctors at AAO Annual Meeting 7Leading Vision Correction Experts in Trusted LASIK Surgeons Directory Educate Fellow Doctors at AAO Annual Meeting 8Leading Vision Correction Experts in Trusted LASIK Surgeons Directory Educate Fellow Doctors at AAO Annual Meeting 9Scott Miller's 'A Call To Arms' And 'Fatal Immunity' Are Available For World-Wide Licensing 2Scott Miller's 'A Call To Arms' And 'Fatal Immunity' Are Available For World-Wide Licensing 3Scott Miller's 'A Call To Arms' And 'Fatal Immunity' Are Available For World-Wide Licensing 4Scott Miller's 'A Call To Arms' And 'Fatal Immunity' Are Available For World-Wide Licensing 5Scott Miller's 'A Call To Arms' And 'Fatal Immunity' Are Available For World-Wide Licensing 6Scott Miller's 'A Call To Arms' And 'Fatal Immunity' Are Available For World-Wide Licensing 7Zyngenia, Inc. Raises $10 Million in Series A Funding 2Zyngenia, Inc. Raises $10 Million in Series A Funding 3Zyngenia, Inc. Raises $10 Million in Series A Funding 4
...ically everybody knows that ERP isnt the rude nois...s and soda. And most of that savvy population also... silver bullet solving your company-wide functiona...not out to shoot that duck in a barrel today. What...nt equal portfolio management. Ive run into severa...
...mega Corporation ,Source: www.promega.com FITCHBU...panies will receive $250,000 each in tax credits t...oyle announced on Tuesday. , ,Placon Corporation, ...l use the tax relief to create 19 new jobs, and bi... officials said. , ,Both companies are in the Cap...
...ther plays in concert. Source: Brother Pricks and ...as didgeridoos and bagpipes, the Australian band ... as mongrel music and emerged as a prolific figur... known in other circles as advocates for research ...uch as diabetes, Parkinsons, and Alzheimers. Curre...
Other Biology Technology:Steering a project portfolio takes more than expensive software 2Steering a project portfolio takes more than expensive software 3Aussie rock band to raise stem-cell awareness with Milwaukee concert 2Aussie rock band to raise stem-cell awareness with Milwaukee concert 3Aussie rock band to raise stem-cell awareness with Milwaukee concert 4
(Date:11/4/2009)...Mass., Oct. 20 Aware, In...nd technology and biometrics software, today repor... September 30, 2009. ,, Michael Tzannes, Awar...arlier today, we announced an agreement to sell ou... a new fabless semiconductor company being spun ou...
(Date:11/4/2009)... Calif., Oct. 23 GenVault Corporat...anagement for genomic discovery, medicine and iden...D., GenVault,s President and CEO, will be presenti...on October 26th and 27th in La Jolla, CA. ,, Du...overview of the company together with recent custo...
(Date:11/4/2009)... Oct. 27 Global Rainmakers, Inc., ...ced R&D laboratory, today announced that the c...ed to actively commercialize its suite of technolo...ecurity offerings. ,, During the past four year...rs to develop and deploy a series of core--but exp...
Breaking Biology News(10 mins):Aware, Inc. Reports Third Quarter 2009 Financial Results 2Aware, Inc. Reports Third Quarter 2009 Financial Results 3Aware, Inc. Reports Third Quarter 2009 Financial Results 4Aware, Inc. Reports Third Quarter 2009 Financial Results 5Aware, Inc. Reports Third Quarter 2009 Financial Results 6Aware, Inc. Reports Third Quarter 2009 Financial Results 7GenVault Corporation to Present at the 2009 BIOCOM Investor Conference 2Global Rainmakers Brings Cutting-Edge Biometric Security Products to Reality 2Global Rainmakers Brings Cutting-Edge Biometric Security Products to Reality 3Nearly half of all US children will use food stamps says poverty expert at WUSTL 60553 1Nearly half of all US children will use food stamps says poverty expert at WUSTL 60553 2Smokers with common autoimmune disorder at higher risk for skin damage 60551 1Smokers with common autoimmune disorder at higher risk for skin damage 60551 2High throughput genotyping protein purification featured in Cold Spring Harbor Protocols 10608 1High throughput genotyping protein purification featured in Cold Spring Harbor Protocols 10608 2
...clerosis (MS) tends to go into remission while wom...e devastating neurodegenerative disease, according...wn that a pregnancy-related hormone is responsible...e cells. , In a paper to be published in the Febru...m of researchers from the U of C,s Faculty of Medi...
...ch for the genetic roots of the most common form o...ns scientists have newly identified 34 unique vari...ed subjects with ALS. , The 34 so-called single nu...ndidate genes predisposing people to the non-inher... , “Although we haven’t located the exact gene r...
...nd the world die of this type of meningitis, accor...rial meningitis, as the disease is called, can eve...ffected in a matter of weeks in the late 1990s. W...ct the disease is 85-90 percent, with treatment so...f serious disability after recovery. , Only humans...
Other Biology News:Pregnancy hormone key to repairing nerve cell damage 2Pregnancy hormone key to repairing nerve cell damage 3Gene hunters close in on Lou Gehrig’s disease 2
Goat Anti-Human SART1 Polyclonal Antibody, Unconjugated from Abcam
Rhodamine-B-isothiocyanate from Research Organics, Inc.
Rabbit Anti-Schizosaccharomyces pombe SpUlp2 Polyclonal Antibody, Unconjugated from Abcam
Anti-Human sRANKL Polyclonal Antibody, Biotin Conjugated from Cell Sciences
Biology Products: