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RNAi Gene Silencing System

Gene silencing by RNA interference (RNAi) in eukaryotic cell cultures requires double-stranded molecules known as short interfering RNA (siRNA). QIAGEN is a licensed supplier of siRNA, offering a custom siRNA oligonucleotide synthesis service, library siRNA directed against common target genes, and a Cancer siRNA Oligo Set for screening a large number of cancer-related genes using RNAi techniques. Features and benefits
  • siRNA synthesis using patented TOM-Amidite chemistry for high-coupling efficiency
  • High-purity siRNA for efficient gene silencing
  • Expert advice on siRNA design for maximizing gene-silencing potential
  • Labeled siRNA allowing transfection to be easily followed
  • siRNA is annealed and ready-to-use in cell transfection
Principle The application of RNAi to mammalian cells has revolutionized the field of functional genomics by allowing targeted inhibition of gene expression using double-stranded RNA (dsRNA) that carries the same sequence as mRNA transcribed from the target gene. In mammalian cell systems, double-stranded siRNA oligos can be used to induce gene silencing.

After siRNAs are delivered to the cytoplasm of a cell, they bind to a multi-protein complex to form an RNA-induced silencing complex (RISC). The siRNA-protein complex targets en
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