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RNAi: The Controls You Need

RNAi effect is reversed through expression of a target gene refractory to silencing by a particular siRNA can give final confirmation that an observed effect is specifically due to knocking down the target of interest. Like array analysis, this experiment is time consuming.

While considered the ultimate controls, editors of Nature Cell Biology and other journals do not require these experiments for publication in their journals.


Controls for RNAi Screening Experiments

Use of many of the above controls in large scale RNAi screens is virtually impossible. At a minimum, one or more negative and positive control siRNAs should be used per plate analyzed. And validation of some or all of the "hits" using more extensive controls is warranted. Finally, the best way to improve confidence in RNAi data is to use at least three siRNAs in independent wells of the screen. See Setting up Successful siRNA Library Screens, for more information about ways to use siRNA libraries for both large and small scale RNAi screens.


As the RNAi Field Evolves

The use of RNAi has enormous potential for analyzing gene function, elucidating biological pathways, and identifying and validating potential drug targets. The RNAi field is still relatively new, and recommendations for experimental design and proper c
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Related biology technology :

1. RNAi: A How To for New Users
2. RNAi: Get the Whole Story
3. RNAi: Size Does Matter
4. Housekeeping Genes: Universal Positive Controls in siRNA Knockdown Experiments
5. Designing Controls for siRNA Experiments
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