Although it is necessary to monitor mRNA levels to validate siRNAs, most researchers also want to determine the extent of knockdown at the protein level. Ambions PARIS Kit (Protein And RNA Isolation System) provides a simple method for isolating total RNA and protein from the same sample. RNA isolated with the kit can be used for RT-PCR, array analysis, Northern blotting, or other analytical techniques, and the protein lysate is compatible with Western blotting. Since native protein is recovered in most cases, enzymatic assays can also be performed.
The most careful of researchers correlate
siRNA, target mRNA, and target protein levels. Isolat