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RNAi: A How To for New Users

ion Agents were designed expressly for this purpose. Delivery into primary cells and suspension cells, however, can be problematic, if not impossible, using standard transfection methodologies. In these cases, electroporation using a specialized, gentle-on-cells buffer and optimized pulsing conditions generally results in very efficient siRNA delivery without compromising cell viability. Ambion's siPORT siRNA Electroporation Buffer is a revolutionary new buffer that makes electroporation an ideal method for delivering siRNAs into primary and other difficult-to-transfect cell types.


Controls for siRNA Experiments
Proper controls are needed for every RNAi experiment. A negative control that does not target any endogenous transcript is needed to control for nonspecific effects on gene expression caused by introducing any siRNA. For the vast majority of experiments, Ambion scientists use and recommend Silencer Negative Control #1 siRNA. This negative control has been extensively tested and proved to have few nonspecific effects in human, mouse, and rat cells. Easy-to-assay positive controls are needed to optimize transfection conditions, ensure that siRNAs are efficiently delivered, and ascertain that a particular downstream assay is working. Since positive cont
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2. RNAi: Size Does Matter
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