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RNAi: A How To for New Users

e preferred and most widely used method of siRNA preparation. siRNAs are easier to transfect than plasmids. More importantly, pre-designed, gene-specific siRNAs in a ready-to-use format are available, which makes this method the easiest and the most likely to succeed.

Ambion, in partnership with Cenix BioScience, provides expert designed, guaranteed-to-silence siRNAs to >34,000 human, mouse, and rat targets (>98% of all human, mouse, and rat genes in the RefSeq database). These siRNAs are available individually as Silencer Pre-designed or Validated siRNAs, and in functional class-focused sets as Silencer siRNA Libraries. Individual siRNAs allow detailed analysis of an individual genes role in one or more pathways, whereas sets of siRNAs (libraries) enable large scale screening experiments to tie genes to cellular function.


Delivery of siRNAs into Cultured Cells
Once you have an siRNA, you need a means to deliver it into your cells. For many immortalized cell lines, transfection with a lipid- or amine-based reagent is the preferred option. Ambion's siPORT Lipid and siPORT Amine Transfect
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