Navigation Links
RNAi: A How To for New Users


RNA interference, the biological mechanism by which double-stranded RNA (dsRNA) induces gene silencing by targeting complementary mRNA for degradation, is revolutionizing the way researchers study gene function. For the first time, scientists can quickly and easily reduce the expression of a particular gene in mammalian cell systems, often by 90% or greater, to analyze the effect that gene has on cellular function. The ease of the technique, as well as the wide availability of high quality kits and reagents for performing RNAi, have contributed to its rapid adoption by the research community. This article provides an overview of RNAi, the requirements for a typical RNAi experiment, and the Ambion products that simplify each step.


The RNAi Mechanism
In non-mammalian systems, introducing or expressing long double-stranded RNA (dsRNA) triggers the RNAi pathway. The cytoplasmic nuclease Dicer first cleaves the long dsRNA into 2123 bp small interfering RNAs (siRNAs), that then unwind and assemble into RNA-induced silencing complexes (RISCs) (Figure 1). The antisense siRNA strand then guides the RISC to complementary RNA molecules, and the RISC cleaves the mRNA, leading to specific gene silencing. However, since most mammalian cells mount a potent antiviral response upon introduction of dsRNA longer than 30 bp, researchers transfect cells with 2123 bp siRNAs to induce RNAi in these systems without eliciting the antiviral response.

Figure 1. Overview of RNA Interference.


'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9

Related biology technology :

1. RNAi: Get the Whole Story
2. RNAi: Size Does Matter
3. RNAi: The Controls You Need
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: RNAi How for New Users

(Date:11/26/2014)... PA (PRWEB) November 25, 2014 The ... of its official conference and exposition mobile app, ... for free download in the App Store for iOS ... a valuable resource tool before, during and after the ... containing details on exhibiting companies, technical sessions, Conferee Networking ...
(Date:11/26/2014)... 2014 CURE™ magazine will ... December 5, 2014, in San Francisco prior to the ... event will honor clinicians, caregivers, and advocates who have ... (MPNs). , Special guest speaker Charles Esten, from ... journey at the reception. Over a decade ago, ...
(Date:11/26/2014)... TOKYO , 25 novembre 2014 /PRNewswrie/ -- Theravalues ... per il mercato europeo in occasione di Hi Europe ... ). Si tratta della curcumina con ... tecnologia amorfa (senza cristalli) e ingredienti approvati dalle norme ... presente nella spezia della pianta curcumina ( Curcuma longa ...
(Date:11/24/2014)... 24, 2014  Last week, Representatives Gus ... (D-NC) took a bold bipartisan step on behalf ... OPEN Act. Original co-sponsors of the legislation include ... will incentivize drug makers and innovators to "repurpose" ... pediatric cancers, which opens the door to the ...
Breaking Biology Technology:Pittcon Releases 2015 Mobile App 2CURE Magazine and Incyte to Honor MPN Heroes 2CURE Magazine and Incyte to Honor MPN Heroes 3CURE Magazine and Incyte to Honor MPN Heroes 4La curcumina con la maggiore biodisponibilità di sempre farà il suo debutto in Europa 2La curcumina con la maggiore biodisponibilità di sempre farà il suo debutto in Europa 3OPEN Act Introduced to Repurpose Drugs for Rare Diseases 2
... Limited (VNRX.OB), a life sciences company focused on developing ... a specialist company spun out from University College London ... biobank of over 5,000,000 human serum samples to validate ... which were collected by scientists at UCL, will be ...
... for Biologically Inspired Engineering at Harvard University have developed ... seek out specific cell targets within a complex mixture ... as telling cancer cells to self-destruct. Inspired by the ... might one day be used to program immune responses ...
... WASHINGTON, Feb. 16, 2012 Senior analysts from The ... on-site in Las Vegas preparing to provide an immediate ... Healthcare Information and Management Systems Society,s HIMSS12 Annual Conference ... (Logo: http://photos.prnewswire.com/prnh/20110802/PH45999LOGO ) ...
Cached Biology Technology:Volition Signs Collaboration Agreement With Abcodia 2Volition Signs Collaboration Agreement With Abcodia 3Harvard's Wyss Institute develops DNA nanorobot to trigger targeted therapeutic responses 2Harvard's Wyss Institute develops DNA nanorobot to trigger targeted therapeutic responses 3Meaningful Use Stage Two: The Advisory Board Company Helps Hospitals Set Strategy 2Meaningful Use Stage Two: The Advisory Board Company Helps Hospitals Set Strategy 3
(Date:11/7/2014)... , November 6, 2014 Leading Biometric ... better mobile security revolutionizing online transactions.  Companies in focus today ... Ltd. (NYSE: BABA ), Google Inc. (NASDAQ: ... eBay Inc. (NASDAQ: EBAY ) and MasterCard Inc. ... and NXTDW), a biometric authentication company focused on the ...
(Date:11/6/2014)... 2014 - Insilico Medicine, Inc, a Baltimore-based bioinformatics ... related diseases announced a research collaboration with the ... Oncology, Inc (OTC: CSBR). , "There are many ... but Champions Oncology,s TumorGraft technology is unique in ... regimen experimentally generating vast amounts of valuable data. ...
(Date:11/4/2014)... of Utah engineers developed the first room-temperature fuel cell ... without needing to ignite the fuel. These new fuel ... power and sensors. , A study of the new ... journal ACS Catalysis . , Fuel cells convert ... fuel and an oxygen-rich source such as air. If ...
Breaking Biology News(10 mins):Universal Authentication Methods Provide More Secure Operations for Biometrics Devices - Company Joins FIDO Alliance for Consumer Protection 2Universal Authentication Methods Provide More Secure Operations for Biometrics Devices - Company Joins FIDO Alliance for Consumer Protection 3Universal Authentication Methods Provide More Secure Operations for Biometrics Devices - Company Joins FIDO Alliance for Consumer Protection 4Universal Authentication Methods Provide More Secure Operations for Biometrics Devices - Company Joins FIDO Alliance for Consumer Protection 5Universal Authentication Methods Provide More Secure Operations for Biometrics Devices - Company Joins FIDO Alliance for Consumer Protection 6Insilico Medicine, Inc announces research collaboration with Champions Oncology, Inc 2Jet-fueled electricity at room temperature 2
... ultimate enzyme responsible for all aerobic life on Earth, ... component of the cellular machinery that generates energy in ... that scientists would have a clear understanding of how ... Collman, professor emeritus of chemistry at Stanford University. , ...
... Researchers at the University of California, Santa Cruz, have determined ... carries out a fundamental reaction required to make new RNA ... the first self-replicating molecule to arise billions of years ago ... In all forms of life known today, the synthesis of ...
... bound in vesicles, miniscule membrane-enclosed packages of proteins and ... vesicle membrane must fuse with another membrane, creating channels ... membrane fusion, is fundamental to health and disease. It ... hormones circulating and brain cells firing. Membrane fusion is ...
Cached Biology News:Researchers create artificial enzyme that mimics the body's internal engine 2Researchers create artificial enzyme that mimics the body's internal engine 3RNA enzyme structure offers a glimpse into the origins of life 2Biologists prove critical step in membrane fusion 2
... media are rigid macroporous hydrophilic media. The ... at moderate pressures, while the macroporous beads ... hydrophilic matrix reduces nonspecific binding. Macro-Prep High ... exchanger with very high flow rates and ...
... collection of phage display libraries, human ... and mouse tissues. All cDNA libraries ... size of cDNA inserts has been ... 1000bp by gel electrophoresis of DNA ...
... is a competitive enzymeimmunoassay (EIA) for the ... rat serum or plasma. Corticosterone is the ... and mice, and in most non-mammalian vertebrates ... mammals produce both corticosterone and cortisol, in ...
Mouse monoclonal [1.F.1] to Yersinia pestis F1 antigen ( Abpromise for all tested applications). Antigen: Purified F1 antigen of Y. pestis vaccine strain EB....
Biology Products: