Navigation Links
RNAPrep - Total RNA Isolation Purification


RNAPrep is a SPRI based magnetic bead purification kit for extracting and purifying total RNA from Eukaryotic cultured cells. RNAPrep is a simple and reproducible 3-step procedure for isolating total RNA for use in applications such as microarray and RT-PCR gene expression analysis. RNAPreps automation friendly format is scalable and consistently delivers high yield recovery and purity without the need for filtration or centrifugation.


RNAPrep Features
Isolation and purification of high quality RNA from cultured cells in tube and 96-well formats.
Efficient removal of contaminating genomic DNA.
No centrifugation, filtration or precipitation required.
Scalable for automation platforms


Application Flexibility
RNA Amplification
qRT-PCR
cDNA synthesis
Northern Blot analysis


Format Flexibility
Tube-based
96-well plates
Manual and Automation friendly


High RNA Yield
Total RNA yield obtained with RNAPrep was compared to two competitor products. RNAPrep routinely produced 1.5-3.0 times higher yields and demonstrated consistent total RNA recovery over a 5-log range (Figures 1A and 1B).

Figure 1A. Total RNA from 1x105 293T cells was isolated with RNAPrep and two other competitor methods, Q & P. Quantitation was performed by RiboGreen assay. RNAPrep yielded 1.5-3.0 times greater yield than competitors Q & P.

Figure 1B. Total RNA isolated from 100 to 1x106 3638C lymphocyte cells was used in a TaqMan RT-PCR assay for the beta-ac tin transcript. Recovery of total RNA using RNAPrep was linear over a 5 log range.


Quality Nucleic Acid Purification
RNAPrep was compared against two competitor kits for isolated total RNA quality. Analysis of samples by the Agilent 2100 bioanalyzer showed RNAPrep isolated RNA had consistently higher RIN scores (Figure 2) that asses 18/28S ribosomal peak highs and ratios along with several other parameters to provide an objective measure of RNA quality on a scale of 1 to 10 (for more information on RIN RNA quality scores refer to Genomics and Proteomics v.4, no.5, pgs. 14-21).

Figure 2. Total RNA was isolated from 1x105 293T cells using (A) RNA Prep, (B) competitor Qs vacuum filtration isolation kit, or (C) competitor Ps magnetic bead total RNA isolation kit. All were analyzed using the Agilent 2100 bioanalyzer. (D) An equal percentage of eluted RNA from each kit was analyzed by agarose gel electrophoresis using a 1.2% E-gel (Invitrogen). (E) Average RNA Integrity Number (RIN) as determined from Agilent 2100 bioanalyzer, reported on a scale of 1-10, 10 being highest quality.


Automation Compatible
RNAPrep can be performed either in single tube or in full automation plate formats, making it completely scalable. The flexibility of SPRI magnetic bead technology allows you to run partial plates without incurring costs from running fewer than 96-wells. RNA quality obtained with an automated protocol
is indicated in Figure 3.


Quality Downstream Performance
RNA isolated with RNAprep is suitable for downstream applications such as RT-PCR and microarray gene expression analys is. In Figure 4, RNA isolated with RNAPrep from 1x105 293T cells was used as a template for RT-PCR of human genes of varying abundance levels.


Summary
RNAPrep is a simple, 3-step total RNA extraction and purification method that delivers superior performance in yielding high-quality RNA for use in applications such as microarrays and RT-PCR gene expression analysis. The kit provides the benefits of SPRI magnetic bead technology that enable efficient removal of unwanted contaminants without the need for filtration or centrifugation. RNAPreps flexibility and scalability allow you to work in single tube or fully automated plate formats.



Available For Use In Your Laboratory
To Place an Order or Receive Technical Assistance:In the U.S. and Canada call us at 800-361-7780.Outside the U.S. and Canada call us at:+1-978-867-2603.Visit us at www.agencourt.com


back to top
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. New Kit Generates Exceptionally Pure Total RNA for RT-PCR
2. High-Throughput Isolation of Total RNA
3. Isolate and Analyze Total RNA from Cells Harvested by Laser Capture Microdissection
4. Fast Isolation of Total RNA from Small Numbers of Cells
5. Total RNA Isolation Protocol 1,2,3
6. Isolate It All: siRNA miRNA Total RNA Native Protein
7. Isolate Total RNA and Protein From the Same Sample
8. Isolate High Quality Total RNA from LCM Samples Suitable for Microarray and qRT-PCR Analysis
9. Recover High Yields of Total Nucleic Acid from Formalin-fixed, Paraffin-embedded (FFPE) Tissue
10. Total RNA from Whole Blood for Expression Profiling
11. Total RNA Isolation From 500 l Body Fluid
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:5/26/2016)... , May 26, 2016  Agriculture nutrients are ... Des Moines, Iowa is running their nitrate ... Lake Erie and coastal regions nationwide ... to preventing this widespread issue. NECi Superior ... Upper Peninsula, developed a new, easy to use ...
(Date:5/26/2016)... (PRWEB) , ... May 26, 2016 , ... ... manufacturing company, today announced several positive developments that position the Company for the ... result of the transaction, Craig F. Kinghorn has been appointed Chairman of the ...
(Date:5/25/2016)... ... 2016 , ... Founder of the Fitzmaurice Hand Institute in ... of the hand by the National Board of Physicians and Surgeons, as of ... in his pursuit of providing the most comprehensive, effective treatment for his patients, ...
(Date:5/25/2016)... ... May 25, 2016 , ... Biohaven Pharmaceutical Holding Company Ltd. ... the company’s orphan drug designation request covering BHV-4157 for the treatment of Spinocerebellar ... FDA. , Spinocerebellar ataxia is a rare, debilitating neurodegenerative disorder that is ...
Breaking Biology Technology:
(Date:4/15/2016)... , April 15, 2016  A new partnership ... more accurate underwriting decisions in a fraction of ... competitively priced and high-value life insurance policies to ... With Force Diagnostics, rapid testing (A1C, ... data readings (blood pressure, weight, pulse, BMI, and ...
(Date:3/31/2016)...   LegacyXChange, Inc. ... LegacyXChange is excited to release its first ... be launched online site for trading 100% guaranteed authentic ... also provide potential shareholders a sense of the value ... industry that is notorious for fraud. The video is ...
(Date:3/22/2016)... 2016 According to ... for Consumer Industry by Type (Image, Motion, Pressure, ... & IT, Entertainment, Home Appliances, & Wearable ... 2022", published by MarketsandMarkets, the market for ... USD 26.76 Billion by 2022, at a ...
Breaking Biology News(10 mins):