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RNAPrep - Total RNA Isolation Purification


RNAPrep is a SPRI based magnetic bead purification kit for extracting and purifying total RNA from Eukaryotic cultured cells. RNAPrep is a simple and reproducible 3-step procedure for isolating total RNA for use in applications such as microarray and RT-PCR gene expression analysis. RNAPreps automation friendly format is scalable and consistently delivers high yield recovery and purity without the need for filtration or centrifugation.


RNAPrep Features
Isolation and purification of high quality RNA from cultured cells in tube and 96-well formats.
Efficient removal of contaminating genomic DNA.
No centrifugation, filtration or precipitation required.
Scalable for automation platforms


Application Flexibility
RNA Amplification
qRT-PCR
cDNA synthesis
Northern Blot analysis


Format Flexibility
Tube-based
96-well plates
Manual and Automation friendly


High RNA Yield
Total RNA yield obtained with RNAPrep was compared to two competitor products. RNAPrep routinely produced 1.5-3.0 times higher yields and demonstrated consistent total RNA recovery over a 5-log range (Figures 1A and 1B).

Figure 1A. Total RNA from 1x105 293T cells was isolated with RNAPrep and two other competitor methods, Q & P. Quantitation was performed by RiboGreen assay. RNAPrep yielded 1.5-3.0 times greater yield than competitors Q & P.

Figure 1B. Total RNA isolated from 100 to 1x106 3638C lymphocyte cells was used in a TaqMan RT-PCR assay for the beta-ac
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