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RNAClean - RNA & cDNA in vitro Reaction Purification

rity. RNA was incubated at varying temperatures following RNAClean purification for up to 2 days and analyzed using an Agilent 2100 Bioanalyzer to detect RNA degradation (Figure 3). There were no signs of degradation even after a 2 hour incubation at 37 C.

Recovery from Low Concentrations
The RNAClean method is a versatile technique that provides efficient recovery from a wide range of starting RNA concentrations. A 1.9 kb RNA fragment was produced via in vitro transcription and purified using RNAClean reagent (Figure 4). The concentration of the RNA transcript prior to purification ranged from ~100 to 1 ng/L. Recovery results demonstrate that RNAClean efficiently recovers nearly 100% down to 13 ng/L, and was capable of slightly over 60% recovery at concentrations as low as 2 ng/L.


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