Navigation Links
RNAClean - RNA & cDNA in vitro Reaction Purification


Agencourts RNAClean kit provides a simple, flexible and highly reproducible method for purifying nucleic acid products generated in common enzymatic RT and IVT reactions such as cDNA and cRNA. The kit is formatted for microarray gene expression analysis experiments and is easily performed in manual or automated formats. RNAClean eliminates the need for vacuum filtration or centrifugation, and delivers superior nucleic acid yield and purity.


Key Benefits
Minimize required starting material: use as little as 1 g of total RNA for Affymetrix GeneChip gene expression experiments.
Easy and fast protocol: purification performed in less than 20 minutes.
Application flexibility: high quality, efficient recovery of small to large size nucleic acid fragments.
Formatted for Affymetrix GeneChip and Eberwine-based RNA amplification methods.


High Yield and Consistent Recovery
5 g of input total RNA was isolated and processed in the standard Affymetrix GeneChip single round amplification protocol. RNAClean purification was compared to Qiagens MinElute (cDNA) and RNeasy (rRNA) column-based filtration methods. The results show that RNAClean delivers consistently higher yields of the final cRNA product (Figure 1). RNAClean recovery of RNA fragments with sizes ranging from ~155 bp9.6 kb was assessed using gel electrophoresis (Figure 2 and Table 1). RNAClean showed higher recovery rates of both small and large RNAs.


RNA Quality
RNA purified using RNAClean is free of nucleases and other contaminates that can affect downstream data integ rity. RNA was incubated at varying temperatures following RNAClean purification for up to 2 days and analyzed using an Agilent 2100 Bioanalyzer to detect RNA degradation (Figure 3). There were no signs of degradation even after a 2 hour incubation at 37 C.

Recovery from Low Concentrations
The RNAClean method is a versatile technique that provides efficient recovery from a wide range of starting RNA concentrations. A 1.9 kb RNA fragment was produced via in vitro transcription and purified using RNAClean reagent (Figure 4). The concentration of the RNA transcript prior to purification ranged from ~100 to 1 ng/L. Recovery results demonstrate that RNAClean efficiently recovers nearly 100% down to 13 ng/L, and was capable of slightly over 60% recovery at concentrations as low as 2 ng/L.


back to top
'"/>

Source:


Page: All 1 2

Related biology technology :

1. RNA & cDNA Purification Using RNAClean
2. GeneChip Sample Cleanup Module for cleanup of cDNA and in vitro transcribed cRNA
3. Analysis of in vitro micronucleus assays using the IN Cell Analyzer 3000 Micronuclei Formation Analysis Module
4. XL10-Gold Cells Supplied in New Single-Reaction Format
5. Optimizing pfuturbo DNA Polymerase Amplification Reactions with Perfect Match PCR Enhancer
6. High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli
7. Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels
8. Yeast Protein Production System Features High Yields and One-Step Purification
9. Expression and Purification of Recombinant Proteins That Have Native Amino Acid Sequence
10. Easy Cosmid DNA Purification with StrataPrep Kits
11. MinElute PCR Purification Kit
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:6/27/2016)... 27, 2016  Sequenom, Inc. (NASDAQ: SQNM ... lives through the development of innovative products and services, ... the United States denied its petition to ... of Sequenom,s U.S. Patent No. 6,258,540 (",540 Patent") are ... by the Supreme Court,s Mayo Collaborative Services v. Prometheus ...
(Date:6/27/2016)... Raleigh, NC (PRWEB) , ... June 27, 2016 ... ... a mission to bring innovative medical technologies, services and solutions to the healthcare ... development and implementation of various distribution, manufacturing, sales and marketing strategies that are ...
(Date:6/27/2016)... BOSTON , June 27, 2016   Ginkgo ... biology to industrial engineering, was today awarded as ... a selection of the world,s most innovative companies. ... at scale for the real world in the ... organism engineers work directly with customers including Fortune ...
(Date:6/24/2016)... , ... June 24, 2016 , ... While the majority ... as the Cary 5000 and the 6000i models are higher end machines that use ... height of the spectrophotometer’s light beam from the bottom of the cuvette holder. ...
Breaking Biology Technology:
(Date:4/28/2016)... 28, 2016 First quarter 2016:   ... compared with the first quarter of 2015 The gross ... M (loss: 18.8) and the operating margin was 40% (-13) ... Cash flow from operations was SEK 249.9 M (21.2) , ... is unchanged, SEK 7,000-8,500 M. The operating margin for ...
(Date:4/15/2016)... 2016  A new partnership announced today will ... decisions in a fraction of the time it ... high-value life insurance policies to consumers without requiring ... Force Diagnostics, rapid testing (A1C, Cotinine and HIV) ... pressure, weight, pulse, BMI, and activity data) available ...
(Date:3/31/2016)... 2016   LegacyXChange, ... "Company") LegacyXChange is excited to release its ... to be launched online site for trading 100% guaranteed ... will also provide potential shareholders a sense of the ... an industry that is notorious for fraud. The video ...
Breaking Biology News(10 mins):