Navigation Links
RNAClean - RNA & cDNA in vitro Reaction Purification


Agencourts RNAClean kit provides a simple, flexible and highly reproducible method for purifying nucleic acid products generated in common enzymatic RT and IVT reactions such as cDNA and cRNA. The kit is formatted for microarray gene expression analysis experiments and is easily performed in manual or automated formats. RNAClean eliminates the need for vacuum filtration or centrifugation, and delivers superior nucleic acid yield and purity.


Key Benefits
Minimize required starting material: use as little as 1 g of total RNA for Affymetrix GeneChip gene expression experiments.
Easy and fast protocol: purification performed in less than 20 minutes.
Application flexibility: high quality, efficient recovery of small to large size nucleic acid fragments.
Formatted for Affymetrix GeneChip and Eberwine-based RNA amplification methods.


High Yield and Consistent Recovery
5 g of input total RNA was isolated and processed in the standard Affymetrix GeneChip single round amplification protocol. RNAClean purification was compared to Qiagens MinElute (cDNA) and RNeasy (rRNA) column-based filtration methods. The results show that RNAClean delivers consistently higher yields of the final cRNA product (Figure 1). RNAClean recovery of RNA fragments with sizes ranging from ~155 bp9.6 kb was assessed using gel electrophoresis (Figure 2 and Table 1). RNAClean showed higher recovery rates of both small and large RNAs.


RNA Quality
RNA purified using RNAClean is free of nucleases and other contaminates that can affect downstream data integ rity. RNA was incubated at varying temperatures following RNAClean purification for up to 2 days and analyzed using an Agilent 2100 Bioanalyzer to detect RNA degradation (Figure 3). There were no signs of degradation even after a 2 hour incubation at 37 C.

Recovery from Low Concentrations
The RNAClean method is a versatile technique that provides efficient recovery from a wide range of starting RNA concentrations. A 1.9 kb RNA fragment was produced via in vitro transcription and purified using RNAClean reagent (Figure 4). The concentration of the RNA transcript prior to purification ranged from ~100 to 1 ng/L. Recovery results demonstrate that RNAClean efficiently recovers nearly 100% down to 13 ng/L, and was capable of slightly over 60% recovery at concentrations as low as 2 ng/L.


back to top
'"/>

Source:


Page: All 1 2

Related biology technology :

1. RNA & cDNA Purification Using RNAClean
2. GeneChip Sample Cleanup Module for cleanup of cDNA and in vitro transcribed cRNA
3. Analysis of in vitro micronucleus assays using the IN Cell Analyzer 3000 Micronuclei Formation Analysis Module
4. XL10-Gold Cells Supplied in New Single-Reaction Format
5. Optimizing pfuturbo DNA Polymerase Amplification Reactions with Perfect Match PCR Enhancer
6. High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli
7. Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels
8. Yeast Protein Production System Features High Yields and One-Step Purification
9. Expression and Purification of Recombinant Proteins That Have Native Amino Acid Sequence
10. Easy Cosmid DNA Purification with StrataPrep Kits
11. MinElute PCR Purification Kit
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:7/24/2017)... ... July 24, 2017 , ... ... the stock market news outlet had initiated coverage on Interpace Diagnostics. Interpace ... and identifies exposure, progression and risk analysis from specific cancers in humans. ...
(Date:7/20/2017)... ... 20, 2017 , ... Resoundant, Inc. is pleased to introduce ... the U.S. that offer MR Elastography for liver fibrosis staging. MR Elastography ... staging liver fibrosis assessment. , “MRE:connect was created in response to an increasing ...
(Date:7/20/2017)... ... 20, 2017 , ... Litmus Health , a clinical ... advisory board. The board comprises leaders spanning business, technology, academia, and pharmaceutical research. ... of Engineering, to Chief Technology Officer. Crooks will lead strategy and development of ...
(Date:7/20/2017)... ... July 20, 2017 , ... Corista, a leader in integrated ... Tuesday, July 25, during the Association of Pathology Chairs’ Jubilee Meeting in Washington, ... Baras, Associate Director of Pathology Informatics, will present “The Digital Pathology Experience at ...
Breaking Biology Technology:
(Date:3/24/2017)... -- The Controller General of Immigration from Maldives Mr. ... have received the prestigious international IAIR Award for the most innovative high ... ... Maldives Immigration Controller ... (small picture on the right) have received the IAIR award for the ...
(Date:3/23/2017)... , March 23, 2017 The report "Gesture Recognition and ... Industry, and Geography - Global Forecast to 2022", published by MarketsandMarkets, the market ... CAGR of 29.63% between 2017 and 2022. Continue ... ... ...
(Date:3/22/2017)... March 21, 2017   Neurotechnology , a ... technologies, today announced the release of the ... provides improved facial recognition using up to 10 ... single computer. The new version uses deep neural-network-based ... and it utilizes a Graphing Processing Unit (GPU) ...
Breaking Biology News(10 mins):