Navigation Links
RNAClean - RNA & cDNA in vitro Reaction Purification


Agencourts RNAClean kit provides a simple, flexible and highly reproducible method for purifying nucleic acid products generated in common enzymatic RT and IVT reactions such as cDNA and cRNA. The kit is formatted for microarray gene expression analysis experiments and is easily performed in manual or automated formats. RNAClean eliminates the need for vacuum filtration or centrifugation, and delivers superior nucleic acid yield and purity.


Key Benefits
Minimize required starting material: use as little as 1 g of total RNA for Affymetrix GeneChip gene expression experiments.
Easy and fast protocol: purification performed in less than 20 minutes.
Application flexibility: high quality, efficient recovery of small to large size nucleic acid fragments.
Formatted for Affymetrix GeneChip and Eberwine-based RNA amplification methods.


High Yield and Consistent Recovery
5 g of input total RNA was isolated and processed in the standard Affymetrix GeneChip single round amplification protocol. RNAClean purification was compared to Qiagens MinElute (cDNA) and RNeasy (rRNA) column-based filtration methods. The results show that RNAClean delivers consistently higher yields of the final cRNA product (Figure 1). RNAClean recovery of RNA fragments with sizes ranging from ~155 bp9.6 kb was assessed using gel electrophoresis (Figure 2 and Table 1). RNAClean showed higher recovery rates of both small and large RNAs.


RNA Quality
RNA purified using RNAClean is free of nucleases and other contaminates that can affect downstream data integ
'"/>

Source:


Page: All 1 2

Related biology technology :

1. RNA & cDNA Purification Using RNAClean
2. GeneChip Sample Cleanup Module for cleanup of cDNA and in vitro transcribed cRNA
3. Analysis of in vitro micronucleus assays using the IN Cell Analyzer 3000 Micronuclei Formation Analysis Module
4. XL10-Gold Cells Supplied in New Single-Reaction Format
5. Optimizing pfuturbo DNA Polymerase Amplification Reactions with Perfect Match PCR Enhancer
6. High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli
7. Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels
8. Yeast Protein Production System Features High Yields and One-Step Purification
9. Expression and Purification of Recombinant Proteins That Have Native Amino Acid Sequence
10. Easy Cosmid DNA Purification with StrataPrep Kits
11. MinElute PCR Purification Kit
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: RNAClean RNA cDNA vitro Reaction Purification

(Date:8/28/2014)... City, CA (PRWEB) August 28, 2014 ... sanitation products for the food processing industry, is asking ... a side-by side comparison of the E2 soap they’re ... E2 Sanitizing Foam Soap . Hand hygiene is ... in the food processing environment. Six key criteria are ...
(Date:8/28/2014)... LAFAYETTE, Ind. A new research platform ... properties of tiny structures undergoing stress and ... to improve designs for microelectronics and batteries., ... reveals information about how heating and the ... mechanical properties. Researchers have discussed the merits ...
(Date:8/28/2014)... (PRWEB) August 28, 2014 The ability ... a culture that will allow individuals to perform at ... increasingly important for employers looking to compete in this ... are embracing this trend, as the sector increasingly focuses ... a company, which will have real results on the ...
(Date:8/28/2014)... 2014 Sterlitech is proud to announce ... its stable of products . These additional ... and include membrane filters with surface charges. ... demand for our membrane process testing equipment, and correspondingly, ... sheet membranes,” explains Sterlitech President Mark Spatz. “The ...
Breaking Biology Technology:Best Sanitizers, Inc. Asks Food Industry Professionals: With Fall Harvest On the Way, Is Your E2 Hand Soap Up to the Task? 2New analytical technology reveals 'nanomechanical' surface traits 2New analytical technology reveals 'nanomechanical' surface traits 3Klein Hersh International Leads Philadelphia Area Life Science Industry in Company Culture 2Klein Hersh International Leads Philadelphia Area Life Science Industry in Company Culture 3Sterlitech Corporation Enhances Its Selection of Flat Sheet Membrane Filters 2
... Testing Market, FULLERTON, Calif., March 6 ... announced today that it has launched the ... integrated work cell which,consolidates chemistry and immunoassay ... to the company,s work cell family,combines Beckman ...
... Earlier Stage ... Chronic Kidney Disease Patients, CAMBRIDGE, Mass., March 6 ... launch of the phosphate binder,Renvela(R) (sevelamer carbonate) for dialysis patients, ... additional,approvals for the product., Genzyme has submitted a marketing ...
... SOUTH EASTON, Mass., March 6 Pressure,BioSciences, Inc. (Nasdaq: ... host,a teleconference to discuss its 2007 financial results and ... the teleconference either,by telephone or through a live web-cast. ... Wednesday March 12, 2008, Time: 4:30 p.m. Eastern ...
Cached Biology Technology:Beckman Coulter Releases the High-Volume UniCel(R) DxC 880i Integrated Work Cell 2Genzyme Launches Renvela(R) in the U.S. for Dialysis Patients 2Genzyme Launches Renvela(R) in the U.S. for Dialysis Patients 3Genzyme Launches Renvela(R) in the U.S. for Dialysis Patients 4Genzyme Launches Renvela(R) in the U.S. for Dialysis Patients 5Pressure BioSciences, Inc. to Discuss 2007 Financial Results and Provide Business Update 2
(Date:8/29/2014)... - In a cell,s nucleus, chromosomal DNA is ... an amalgam biologists call chromatin. Until about two ... "sidekick," the mere packing material around which the ... have developed a greater appreciation for how DNA/histone ... from multiple research institutions studying the sequence of ...
(Date:8/28/2014)... app called TX Invasives is now available from the ... Texas at Austin for identifying harmful non-native plant, insect ... in the United States cause about $137 billion in ... crops, clog waterways, kill native plants and more. , ... for reporting where invasive species occur, we,re more likely ...
(Date:8/28/2014)... new study reports that an expansion of marine protected ... key ecological functions. According to investigators from the ... protecting fish have focused on saving the largest numbers ... that provide key and difficult-to-replace ecological functions. ... performed by fish species that also are food for ...
Breaking Biology News(10 mins):'K-to-M' histone mutations: How repressing the repressors may drive tissue-specific cancers 2'K-to-M' histone mutations: How repressing the repressors may drive tissue-specific cancers 3Mobile app makes ID of harmful plants, insects in Texas a snap 2Study finds marine protected areas inadequate for protecting fish and ocean ecology 2
... another reason why you should listen to your mother ... a chemical present at high levels in a precursor ... etc.), helps prevent the severe blistering and skin breakage ... disease epidermolysis bullosa simplex (EBS). The researchers treated ...
... Colorado at Boulder team excavating an ancient Maya village in ... has discovered an ancient field of manioc, the first evidence ... The manioc field was discovered under roughly 10 feet ... been directing the excavation of the ancient village of Ceren ...
... Researchers from Boston University School of Medicine (BUSM) ... HIV disease progression in HIV-infected persons. The study ... Journal of Acquired Immune Deficiency Syndromes., Alcohol use ... on HIV disease progression has been examined in ...
Cached Biology News:Natural chemical found in broccoli helps combat skin blistering disease 2CU-Boulder team discovers first ancient manioc fields in Americas 2CU-Boulder team discovers first ancient manioc fields in Americas 3Study shows link between alcohol consumption and hiv disease progression 2
This kit only detects beta-galactosidase activity at pH 6 in cultured cells andtissue sections. Beta-galactosidase activity at pH 6 ispresent only in senescent cells, and is not found inpresenescent...
... for Mac OS X provides the most ... protein sequence analysis and molecular biology data ... Vector NTI Suite you can store manage ... types of molecular biology data all within ...
SHEEP ANTI BOVINE LACTOPEROXIDASE...
Growth Medium / Media...
Biology Products: