Navigation Links
RNAClean - RNA & cDNA in vitro Reaction Purification


Agencourts RNAClean kit provides a simple, flexible and highly reproducible method for purifying nucleic acid products generated in common enzymatic RT and IVT reactions such as cDNA and cRNA. The kit is formatted for microarray gene expression analysis experiments and is easily performed in manual or automated formats. RNAClean eliminates the need for vacuum filtration or centrifugation, and delivers superior nucleic acid yield and purity.


Key Benefits
Minimize required starting material: use as little as 1 g of total RNA for Affymetrix GeneChip gene expression experiments.
Easy and fast protocol: purification performed in less than 20 minutes.
Application flexibility: high quality, efficient recovery of small to large size nucleic acid fragments.
Formatted for Affymetrix GeneChip and Eberwine-based RNA amplification methods.


High Yield and Consistent Recovery
5 g of input total RNA was isolated and processed in the standard Affymetrix GeneChip single round amplification protocol. RNAClean purification was compared to Qiagens MinElute (cDNA) and RNeasy (rRNA) column-based filtration methods. The results show that RNAClean delivers consistently higher yields of the final cRNA product (Figure 1). RNAClean recovery of RNA fragments with sizes ranging from ~155 bp9.6 kb was assessed using gel electrophoresis (Figure 2 and Table 1). RNAClean showed higher recovery rates of both small and large RNAs.


RNA Quality
RNA purified using RNAClean is free of nucleases and other contaminates that can affect downstream data integ
'"/>

Source:


Page: All 1 2

Related biology technology :

1. RNA & cDNA Purification Using RNAClean
2. GeneChip Sample Cleanup Module for cleanup of cDNA and in vitro transcribed cRNA
3. Analysis of in vitro micronucleus assays using the IN Cell Analyzer 3000 Micronuclei Formation Analysis Module
4. XL10-Gold Cells Supplied in New Single-Reaction Format
5. Optimizing pfuturbo DNA Polymerase Amplification Reactions with Perfect Match PCR Enhancer
6. High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli
7. Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels
8. Yeast Protein Production System Features High Yields and One-Step Purification
9. Expression and Purification of Recombinant Proteins That Have Native Amino Acid Sequence
10. Easy Cosmid DNA Purification with StrataPrep Kits
11. MinElute PCR Purification Kit
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: RNAClean RNA cDNA vitro Reaction Purification

(Date:5/23/2013)... Tucson, Ariz. (PRWEB) May 23, 2013 ... world’s first and only FDA, Health Canada and CE ... Glasgow Science Centre in Scotland, which boasts more than ... Total Artificial Heart powered by the Freedom® portable ... one of the most advanced exhibitions of its kind. ...
(Date:5/23/2013)... (PRWEB) May 23, 2013 Bed bugs already ... the pests is still ongoing, a report from kmov.com suggested ... for the bed bug treatment. Meanwhile, to be of help, ... from the costly bed bug exterminator service. , ... that AHA has so far spent $35,000 for ...
(Date:5/23/2013)... 2013 Watlow®, an industry leader ... systems, recently introduced its new catalog, Watlow® Heating ... in designing and manufacturing industrial heaters, Watlow provides ... serve diverse customer needs. , A description of ... beginning of the book. These options allow high ...
(Date:5/23/2013)... May 23, 2013 Just released this month ... coach and author Barbara Roche: “Commit to Confidence: 30 ... Filled with helpful tips and quotes from the fields of ... and tangible exercises that readers can do on their own ... achieve their personal and professional goals. , “My ...
Breaking Biology Technology:SynCardia Total Artificial Heart Featured in $3 Million Exhibition at Glasgow Science Centre 2SynCardia Total Artificial Heart Featured in $3 Million Exhibition at Glasgow Science Centre 3Bed Bug Exterminator Service Could Cost AHA $250K, My Cleaning Products Gives Tips How to Save Apartments from Costly Bed Bug Treatment 2Watlow Introduces Its New Industrial Heater Catalog 2
... , MONROVIA, Calif., June 11 STAAR Surgical Company ... and marketer of minimally invasive ophthalmic products, today announced ... Epiphany Injector System for use with the Affinity(TM) Collamer(R) ... of the Epiphany will usher in a new era ...
... COLLEGEVILLE, Pa., June 11 New data from ... that Wyeth,s (NYSE: WYE ) investigational pneumococcal vaccine, ... potential to provide coverage against the 13 most prevalent serotypes ... death in children younger than five worldwide. , ...
... HONG KONG, June 11 /PRNewswire-Asia/ -- With ... week, Filligent, the Australian-led biotech company,is mobilizing stocks of ... A (H1N1) across Australia. The BioMask is the first ... seconds of contact while retaining,the breathability required by front-line ...
Cached Biology Technology:STAAR Surgical Receives FDA Clearance for the Epiphany(TM) Injector System 2STAAR Surgical Receives FDA Clearance for the Epiphany(TM) Injector System 3New Phase 3 Data Continue to Indicate that Wyeth's Investigational 13-valent Vaccine Has the Potential to Broaden Coverage Against Pneumococcal Disease 2New Phase 3 Data Continue to Indicate that Wyeth's Investigational 13-valent Vaccine Has the Potential to Broaden Coverage Against Pneumococcal Disease 3New Phase 3 Data Continue to Indicate that Wyeth's Investigational 13-valent Vaccine Has the Potential to Broaden Coverage Against Pneumococcal Disease 4New Phase 3 Data Continue to Indicate that Wyeth's Investigational 13-valent Vaccine Has the Potential to Broaden Coverage Against Pneumococcal Disease 5New Phase 3 Data Continue to Indicate that Wyeth's Investigational 13-valent Vaccine Has the Potential to Broaden Coverage Against Pneumococcal Disease 6Australia: Face Mask That Kills Swine Flu Readied for Australian Pandemic Fight 2Australia: Face Mask That Kills Swine Flu Readied for Australian Pandemic Fight 3Australia: Face Mask That Kills Swine Flu Readied for Australian Pandemic Fight 4
(Date:5/22/2013)... human health effects from disposal of millions of ... to recommend stronger government policies to encourage recovery, ... That,s the conclusion of a new paper in ... . , Oladele A. Ogunseitan and colleagues point ... powering everything from smart phones to components in ...
(Date:5/22/2013)... insects and wild plants have slowed in recent years, ... the University of Leeds and the Naturalis Biodiversity Centre ... in the diversity of species in Britain, Belgium and ... the picture brightened markedly after 1990, with a slowdown ... and wild plants. , Professor Bill Kunin, ...
(Date:5/21/2013)... that the nation,s land and water resources could likely ... to 25 billion gallons of algae-based fuel a year ... needs. , The findings come from an in-depth look ... grow significant amounts of algae in large, specially built ... 7 issue of Environmental Science and Technology , ...
Breaking Biology News(10 mins):Encouraging signs for bee biodiversity 2Encouraging signs for bee biodiversity 3Going green: Nation equipped to grow serious amounts of pond scum for fuel 2Going green: Nation equipped to grow serious amounts of pond scum for fuel 3Going green: Nation equipped to grow serious amounts of pond scum for fuel 4
... it turns out, children were not the only ones with ... past holiday season. In a new research report published in ... Biology ( http://www.jleukbio.org ), a team of scientists from ... Children show how a compound from licorice root (glycyrrhizin from ...
... biodegradable nanosized particles that can easily slip through ... deliver a sustained-release medication cargo. , The ... into harmless components, could one day carry life-saving ... conditions, including diseases of the eye, lung, gut ...
... study shows that a bacterial protein may help cocaine addicts ... bacterial enzyme that breaks down cocaine, thereby reducing its addictive ... for treatment of addiction has been limited by its short ... in the Journal of Pharmacology and Experimental Therapeutics ...
Cached Biology News:A trip to the candy store might help ward off rare, but deadly infections 2Biodegradable particles can bypass mucus, release drugs over time 2Biodegradable particles can bypass mucus, release drugs over time 3
Porcine Serum Available Anticoagulants: N-02: Citrate N-04: Heparin (Na) N-06: K2EDTA N-08: Potassium Oxalate N-10: EDTA (Na) N-03: Alsevers N-05: ACD N-07: CPD N-09: K3EDTA N-11...
Rat Normal Multiple Tissue Array 1 slide...
... other RNA precipitation methods in that it does ... the method of choice for removing inhibitors of ... LiCl precipitation may not remove all DNA contamination ... to be used for RT-PCR, it should be ...
...
Biology Products: