Navigation Links
RNA Interference and Gene Silencing: History and Overview

- RNA-induced silencing complex. A nuclease complex, composed of proteins and siRNA (see below), that targets and destroys endogenous mRNAs complementary to the siRNA within the complex.

RNA interference (RNAi) - Post-transcriptional gene silencing (PTGS) induced by the direct introduction of dsRNA. The term "RNA interference" was first used by researchers studying C. elegans.

siRNAs - Small interfering RNAs. Current models of PTGS indicate that these 21-23 nucleotide dsRNAs mediate PTGS. Introduction of siRNAs can induce PTGS in mammalian cells. siRNAs are apparently produced in vivo by cleavage of dsRNA introduced directly or via a transgene or virus. Amplification by an RNA-dependent RNA polymerase (RdRP) may occur in some organisms. siRNAs are incorporated into the RNA-induced silencing complex (RISC), guiding the complex to the homologous endogenous mRNA where the complex cleaves the transcript.


The Biochemical Mechanism of RNAi

So how does injection of dsRNA lead to gene silencing? Many research groups have diligently worked over the last few years to answer this important question. A key finding by Baulcombe and Hamilton provided the first clue. They identified RNAs of ~25 nucleotides in plants undergoing cosuppression that were absent in non-silenced plants. These RNAs were complementary to both the sense and antisense strands of the gene being silenced (24).

Further work in Drosophila using embryo lysates and an in vitro system derived from S2 cells shed more ligh
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Related biology technology :

1. More siRNA Vectors for RNA Interference
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: RNA Interference and Gene Silencing History and Overview

(Date:7/31/2014)... -- Nearly 20,000 medical professionals and healthcare leaders gathered ... Lab Expo in Chicago from ... research and technology that will advance medicine and get ... Wednesday, July 30, more than 19,500 attendees had registered ... Expo, with more than 10,000 of these attendees coming ...
(Date:7/31/2014)... sound-processing part of the mouse brain in a way ... in view. Their imaging technique allows zooming in and ... it enabled the team to watch brain cells light ... which represent a step toward better understanding how our ... journal Neuron . , In the past, researchers ...
(Date:7/31/2014)... Calif. , July 31, 2014 Rogne ... San Francisco Bay Area , has acquired a ... of Oxford,s commercialisation company. The potent anti-inflammatory activity of ... at the University of Oxford,s Sir William ... company, which has raised over $1.5 million in seed ...
(Date:7/31/2014)... , July 31, 2014 Research ... "Global Genomics Market 2014-2018" report to their ... Genomics is the study of the genetic material ... be a strong basis for the discovery and development ... human diseases or health conditions. Genomics is supported by ...
Breaking Biology Technology:Research on Digital Health, Alzheimer's, Ebola Draws Nearly 20,000 Attendees to 2014 AACC Annual Meeting & Clinical Lab Expo 2Research on Digital Health, Alzheimer's, Ebola Draws Nearly 20,000 Attendees to 2014 AACC Annual Meeting & Clinical Lab Expo 3New mapping approach lets scientists zoom in and out as the brain processes sound 2Oxford licenses anti-inflammatory peptides to San Francisco Bay Area start-up 2Oxford licenses anti-inflammatory peptides to San Francisco Bay Area start-up 3Global Genomics Market 2014-2018: Key Vendors are F. Hoffmann-La Roche, Life Technologies, Qiagen and Illumina 2
... Madison, Wis. - The University of Wisconsin System ... patents, but ranks lower in transferring that technology to commercial ... included nearly 500 universities worldwide and more than 200 in ... of Santa Monica, Calif. In terms of number and ...
... soil. He also knows how to build a viable ... his ideas about turning landscape data into packaged products ... everywhere," said Rooney, founder and president of Earth ... the growing power of digital communications, he explained, creates ...
... Wis. - Raghu Ramakrishnan wishes he had a crystal ... driver in business. , ,Ramakrishnan, a vice president and research ... next great driver is search, which he called "the cash ... his preference trumps his hunch, it is user-generated content (combined ...
Cached Biology Technology:UW among top biotech patent producers 2Global information firm reaches diverse markets 2Global information firm reaches diverse markets 3Global information firm reaches diverse markets 4Online communities may be next business market 2Online communities may be next business market 3
(Date:7/30/2014)... at New York University is paving the way ... in civilians and military troops exposed to poisonous ... , An article in the current issue of ... in detoxifying organophosphates, which are compounds commonly used ... was developed by NYU School of Engineering Associate ...
(Date:7/30/2014)... Carroll,s Through the Looking Glass, collaborators from the ... improved a 35-year-old ecology model to better understand ... years. , The new model, called a mean ... Effect," an evolutionary hypothesis introduced by Lee Van ... must constantly increase their fitness (or ability to ...
(Date:7/30/2014)... that David L. Kaplan, Ph.D., will head the brand-new, ... as editor-in-chief. With the first issue slated for publication ... research in the rapidly burgeoning field of biomaterials, the ... with living or biological systems. , "This new journal ... the field of biomaterials over the past decade and ...
Breaking Biology News(10 mins):Engineering a protein to prevent brain damage from toxic agents 2Classic Lewis Carroll character inspires new ecological model 2Classic Lewis Carroll character inspires new ecological model 3ACS Biomaterials Science & Engineering: Brand-new journal launches in 2015, names editor 2
... Children,s National Medical Center have discovered that ... are significantly more likely to have low levels of ... This study supports recent research that suggests vitamin D ... keeping bones healthy. Vitamin D deficiency has been recently ...
... March 17 Orbel,s innovative Board Shield ... PCB shield. Visit the BLS section   ... variety of surface mount or through-hole configurations. Then click ... lets you enter shield dimensions, mounting style, and ...
... In the 1930s, soil used as ballast to weigh down ... red imported fire ant to the southern United States. Since ... Maryland and as far west as California, shorting out streetlights ... Plant pests like the fire ant cost the U.S. ...
Cached Biology News:Study: Low levels of vitamin D linked to higher rates of asthma in African-American kids 2Orbel Introduces the Board Shielding Wizard 2From international harbor to native habitat 2From international harbor to native habitat 3
Silica bead method is an economic method of DNA extraction from agarose...
BD IMag Anti-Mouse CD90.2 (Thy1.2) Particles-MSC 2 x 109 cells...
AgarPlaque Plus Agarose 50 grams...
...
Biology Products: