Navigation Links
RNA Interference and Gene Silencing: History and Overview

- RNA-induced silencing complex. A nuclease complex, composed of proteins and siRNA (see below), that targets and destroys endogenous mRNAs complementary to the siRNA within the complex.

RNA interference (RNAi) - Post-transcriptional gene silencing (PTGS) induced by the direct introduction of dsRNA. The term "RNA interference" was first used by researchers studying C. elegans.

siRNAs - Small interfering RNAs. Current models of PTGS indicate that these 21-23 nucleotide dsRNAs mediate PTGS. Introduction of siRNAs can induce PTGS in mammalian cells. siRNAs are apparently produced in vivo by cleavage of dsRNA introduced directly or via a transgene or virus. Amplification by an RNA-dependent RNA polymerase (RdRP) may occur in some organisms. siRNAs are incorporated into the RNA-induced silencing complex (RISC), guiding the complex to the homologous endogenous mRNA where the complex cleaves the transcript.


The Biochemical Mechanism of RNAi

So how does injection of dsRNA lead to gene silencing? Many research groups have diligently worked over the last few years to answer this important question. A key finding by Baulcombe and Hamilton provided the first clue. They identified RNAs of ~25 nucleotides in plants undergoing cosuppression that were absent in non-silenced plants. These RNAs were complementary to both the sense and antisense strands of the gene being silenced (24).

Further work in Drosophila using embryo lysates and an in vitro system derived from S2 cells shed more ligh
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Related biology technology :

1. More siRNA Vectors for RNA Interference
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: RNA Interference and Gene Silencing History and Overview

(Date:9/6/2008)... A free paper helps visitors to electronica ...ultilingual help, electronica information availabl...rmation useful in planning a trip, online registra...es and events. It includes an extensive list of we... , Munich, Germany (PRWEB...
(Date:9/5/2008)...-- With the publication of a study led by Yuntao W...s Department of Molecular and Microbiology, the me...g how the human immunodeficiency virus (HIV) attac...ed by HIV, affected more than 33 million people wo...tion statistics. , In the Sept. 5 issue of the j...
(Date:9/5/2008)...t pilots push planes to explore their limits, rese... Technology (NIST) are probing the newest microsco...acy at the nanoscale. Better nanoscale measurement... production in the semiconductor and nanomanufactu...y uses helium ions to generate the signal used to ...
(Date:9/5/2008)...ood & Drug Administration (FDA) issued in July 200...t acknowledged that nanoscale materials potentiall... the agency and that those materials present chall...vant to product safety and effectiveness may chang...pating the potential for rapid commercialization i...
Breaking Biology Technology:Free White Paper Now Available: How to get the most from your visit to electronica 2008 in Munich 2Free White Paper Now Available: How to get the most from your visit to electronica 2008 in Munich 3Researchers decode viral process that prepares cells for HIV infection 2NIST studies how new helium ion microscope measures up 2Nanotechnology and the FDA: size matters 2
...ry Welwyn, 40 Broadwater Road, Welwyn , G...nalytical Sciences, , Syngenta, Jealotts ...kshire, , RG42 6EY, UK, , ...Bio-Rad PROTEAN Plus Dodeca cell (Figure 1) for ,...rative protein loads. The Dodeca , cell w...
...Seggerson 2 , 1 Tech/Aid, , 7700 Edgewat...boratories, , Inc., 2000 Alfred Nobel Dri...Introduction , Running PCR samples on a...re. , It is common to hand cast a horizon...el of such large size is difficult to cast, handle...
, , , , back to top
... Amplification of DNA through the polymerase c...ve method capable of copying millions of DNA molec...cause of the extreme sensitivity of this method, ,...ctions can result in false positives , or...ded by using positive , displacement pipe...
Other Biology Technology:Use of the PROTEAN Plus Dodeca Cell for Second-Dimension SDS-PAGE, Rev A 2Use of the PROTEAN Plus Dodeca Cell for Second-Dimension SDS-PAGE, Rev A 3Use of the PROTEAN Plus Dodeca Cell for Second-Dimension SDS-PAGE, Rev A 4Use of the PROTEAN Plus Dodeca Cell for Second-Dimension SDS-PAGE, Rev A 5Advantages of ReadyAgarose Precast Gels Over 96-Well Handcast Gels for High-Throughput Analysis, Rev A 2Advantages of ReadyAgarose Precast Gels Over 96-Well Handcast Gels for High-Throughput Analysis, Rev A 3Advantages of ReadyAgarose Precast Gels Over 96-Well Handcast Gels for High-Throughput Analysis, Rev A 4Inactivation of Contaminating Templates in PCR Using High Intensity Ultraviolet Light 2Inactivation of Contaminating Templates in PCR Using High Intensity Ultraviolet Light 3
(Date:9/4/2008)...5 September 2008 Even scientists define ,a gene, ...that the media also have various ways of framing t...earing in the October 2008 issue of EMBO reports ...nalysis of 300 articles in British and Norwegian n...l from the UK; and Aftenposten, Dagbladet, and VG ...
(Date:9/4/2008)...Sept. 4, 2008 As the anniversary of the September...ety (ACS) has issued a new podcast describing an a...y and national security. The podcast is the sixth...mistry Solutions )series. , With new episodes po...nting problems facing society in the 21st Century,...
(Date:9/4/2008)...uter model help stabilize the tuna population? Can...out jeopardizing food production? , Those and ot...ts, applied mathematicians, economists, biologists...ell University,s new Institute for Computational S...rant from the National Science Foundation (NSF). T...
(Date:9/4/2008)...ass., Sept. 4, 2008 Declaring the success of an u...y, Los Angeles-based philanthropists Eli and Edyth...heir total gift to the Broad Institute of Harvard ...gifts totaling $200 million, the Broads today gave...tute, which had been launched as a 10-year "ventur...
Breaking Biology News(10 mins):What is a gene? 2New 'chemical radar' among national security innovations in ACS podcast 2New 'chemical radar' among national security innovations in ACS podcast 3Cornell gets $10 million NSF grant to establish new sustainability institute 2Eli and Edythe L. Broad make unprecedented gift to endow Broad Institute of Harvard & MIT 2Eli and Edythe L. Broad make unprecedented gift to endow Broad Institute of Harvard & MIT 3Eli and Edythe L. Broad make unprecedented gift to endow Broad Institute of Harvard & MIT 4Eli and Edythe L. Broad make unprecedented gift to endow Broad Institute of Harvard & MIT 5Touch Bionics a 2008 da Vinci Award winner 6828 1Touch Bionics a 2008 da Vinci Award winner 6828 2New Data Highlight Positive Results of Namenda 28R 29 28memantine HCl 29 Once Daily Extended Release Formulation 2750 1New Data Highlight Positive Results of Namenda 28R 29 28memantine HCl 29 Once Daily Extended Release Formulation 2750 2New Data Highlight Positive Results of Namenda 28R 29 28memantine HCl 29 Once Daily Extended Release Formulation 2750 3New Data Highlight Positive Results of Namenda 28R 29 28memantine HCl 29 Once Daily Extended Release Formulation 2750 4New study shows calcium significantly improves childrens bone health 24668 1New study shows calcium significantly improves childrens bone health 24668 2Geecf 3A Spectrum Blue Steel Announces Strategic Partnership with Al Manhal International Group of Abu Dhabi to Deploy Biosphere Facilities 6825 1Geecf 3A Spectrum Blue Steel Announces Strategic Partnership with Al Manhal International Group of Abu Dhabi to Deploy Biosphere Facilities 6825 2
... Though most rabies fatalities in the United State... for raccoon rabies. In 1990, raccoons topped the ...ing the spread of rabies depends on predicting the...eaks might occur and how the virus might spread. I...ine journal PLoS Biology, Real and colleagues appl...
... This press release isn,t very clear, especially ...to read the ,note, part at the end if you want to ..., Switzerland and London, United Kingdom, February...IV/AIDS today announced that a phase I clinical tr...NA-HIV-C and NYVAC-HIV-C for the prevention of HIV...
... A recently published article in Environmental Mic...e Galician shore is readily able to degrade crude ... Zaidín (Spanish Council for Research, CSIC) in Gr...fter the Prestige oil spill in November 2002. , Af...chemical processes such as evaporation or photoche...
... For patients with cancer of the mouth and throat,...leading to speech and swallowing dysfunction and e...of Pennsylvania School of Medicine,s Department of...ompleted two studies ?the most comprehensive and l... of the daVinci Surgical Robotic System to perform...
Other Biology News:Rabies spread speeds up 2'EuroVacc 02' HIV Vaccine Trial Begins 2'EuroVacc 02' HIV Vaccine Trial Begins 3A hope for oil spill bioremediation 2Two new retroviruses—transmitted from animals—identified 2Two new retroviruses—transmitted from animals—identified 3
Taq DNA Polymerase (T. aquaticus), 250 units. For PCR and other procedures requiring DNA polymerase activity at elevated temperatures. Category: Nucleotides & Enzymes & Biochemicals, Modifying Enzyme
...units/µL ,• High specific activity an...Leaves an 'A' overhang ,• Processes ,A...igh activity in primer extension and other molecul...the enzyme, each lot has been functionally tested ...
Recombinant DNA polymerase from Thermus aquaticus expressed in E. coli; 5 units/L; 1 tube of 1, 000 units enzyme/tube w/Buffer II and MgCl2
...units/µL ,• High specific activity an...Leaves an 'A' overhang ,• Processes ,A ...gh activity in primer extension and other molecula...he enzyme, each lot has been functionally tested f...
Biology Products: