Navigation Links
RNA Interference and Gene Silencing: History and Overview

Caenorhabditis elegans. Seven years ago, researchers Guo and Kemphues were attempting to use antisense RNA to shut down expression of the par-1 gene in order to assess its function. As expected, injection of the antisense RNA disrupted expression of par-1, but quizzically, injection of the sense-strand control did too (9).

This result was a puzzle until three years later. It was then that Fire and Mello first injected dsRNA a mixture of both sense and antisense strands into C. elegans (10). This injection resulted in much more efficient silencing than injection of either the sense or the antisense strands alone. Indeed, injection of just a few molecules of dsRNA per cell was sufficient to completely silence the homologous gene's expression. Furthermore, injection of dsRNA into the gut of the worm caused gene silencing not only throughout the worm, but also in its first generation offspring (10).

The potency of RNAi inspired Fire and Timmons to try feeding nematodes bacteria that had been engineered to express dsRNA homologous to the C. elegans unc-22 gene. Surprisingly, these worms developed an unc-22 null-like phenotype (11-13). Further work showed that soaking worms in dsRNA was also able to induce silencing (14). These strategies, whereby large numbers of nematodes are exposed to dsRNA, have enabled large-scale screens to select for RNAi-defective C. elegans mutants and have led to large numbers of gene knockout studies within this organism (15-18).

RNAi in Drosophila
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Related biology technology :

1. More siRNA Vectors for RNA Interference
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: RNA Interference and Gene Silencing History and Overview

(Date:7/1/2015)... ... ... The next-generation sequencing (NGS) clinical market is poised for huge growth as it ... reveals in its new report that the market drivers of this vibrant industry include ... better diagnostics as part of a molecular diagnostics trend. , The global market is ...
(Date:7/1/2015)... July 1, 2015  Axovant Sciences Ltd. (NYSE: ... the treatment of dementia, today announced that the ... 22 at 5:45 p.m. EDT following its presentation ... International Conference 2015 (AAIC). The ... , Dr. Lawrence Friedhoff , Chief Development ...
(Date:7/1/2015)... -- InferMed , s ... will augment ... olutions suite   Elsevier , a ... services, announced today the acquisition of  InferMed , a ... InferMed,s Arezzo technology supports clinicians in choosing the ...
(Date:7/1/2015)... YORK , July 1, 2015  UCB, ... G ), a global leader in designing, transforming, ... Aecus Innovation Award in recognition of their close ... efficiencies in UCB,s global business services (GBS) finance ... service providers and clients that work together effectively ...
Breaking Biology Technology:NGS-Based Diagnostics, the ‘Must-Have Platform’, Sends Market Segment CAGRs Soaring According to BCC Research 2NGS-Based Diagnostics, the ‘Must-Have Platform’, Sends Market Segment CAGRs Soaring According to BCC Research 3Axovant Sciences To Host Investor Briefing And Live Webcast During AAIC 2015 Meeting 2Axovant Sciences To Host Investor Briefing And Live Webcast During AAIC 2015 Meeting 3Elsevier Acquires InferMed, Provider of Clinical Decision Support Technology 2Elsevier Acquires InferMed, Provider of Clinical Decision Support Technology 3Elsevier Acquires InferMed, Provider of Clinical Decision Support Technology 4UCB and Genpact Receive Aecus Innovation Award for Success in Global Business Services 2UCB and Genpact Receive Aecus Innovation Award for Success in Global Business Services 3UCB and Genpact Receive Aecus Innovation Award for Success in Global Business Services 4UCB and Genpact Receive Aecus Innovation Award for Success in Global Business Services 5
... , ... Bioinformatics Solution Provider to Support High Throughput Molecular Data Analysis and Result Sharing ... ... 2010 -- Genedata, a leading provider of advanced software solutions for drug discovery ...
... the airbag in your car to inflate and rotate ... couch. Now a research group of Tel Aviv University,s ... useful. Prof. Yael Hanein, Dr. Slava Krylov and ... make sensors for microelectromechanical systems (MEMS) significantly more sensitive ...
... ... Advanced Plant Genetics to Help Farmers Boost Agricultural Productivity , ... Des Moines, Iowa (Vocus) March 18, 2010 -- DuPont ... DuPont business Pioneer Hi-Bred will expand its presence in central Iowa with ...
Cached Biology Technology:Genedata Announces Five-Year Agreement Extension with Kluyver Centre for Genomics of Industrial Fermentation 2Genedata Announces Five-Year Agreement Extension with Kluyver Centre for Genomics of Industrial Fermentation 3Genedata Announces Five-Year Agreement Extension with Kluyver Centre for Genomics of Industrial Fermentation 4A more sensitive sensor 2DuPont to Expand Plant Genetics Research Facilities 2DuPont to Expand Plant Genetics Research Facilities 3
(Date:6/24/2015)... June 24, 2015 This report provides market ... next six years. It contains an analysis of the ... with their impact from the short, medium, and long ... the industry, market, and technology trends that currently, prevail ... of concerned authorities to efficiently manage the increasing marine ...
(Date:6/23/2015)... 2015 Research and ... the "Body-Worn Temperature Sensors Market - Global ... 2014 - 2020" report to their offering. ... the global body-worn temperature sensors market. The global ... the basis of types, care setting, patient demographic, ...
(Date:6/17/2015)... HILDEN , Deutschland und GERMANTOWN, Maryland ... Investigator ®   Produktlinie mit ... QIAGEN N.V. (NASDAQ: QGEN ; Frankfurt Prime ... neue STR-Testkits der Produktlinie Investigator ... DNA-Spuren in den Markt eingeführt. Die neuen Kits zur ...
Breaking Biology News(10 mins):Automatic Identification System Market by Class, by Platform, by Application and by Geography - Global Forecasts & Analysis to 2014 - 2020 2Body-Worn Temperature Sensors Market - Global Industry Analysis 2QIAGEN bringt in den USA neue Kits für die forensische DNA-Analyse auf den Markt 2QIAGEN bringt in den USA neue Kits für die forensische DNA-Analyse auf den Markt 3QIAGEN bringt in den USA neue Kits für die forensische DNA-Analyse auf den Markt 4QIAGEN bringt in den USA neue Kits für die forensische DNA-Analyse auf den Markt 5QIAGEN bringt in den USA neue Kits für die forensische DNA-Analyse auf den Markt 6
... has reviewed data from studies of controlling human-caused algae ... the nutrient phosphorus is the key to fighting the ... human input of both nitrogen and phosphorus into lakes ... contrast, Schindler concluded that only phosphorus control is necessary ...
... MINNEAPOLIS / ST. PAUL (08/22/2012) The University of Minnesota ... of Energy (DOE) totaling $13.1 million over the next ... the Department of Chemistry in the University,s College of ... will include developing new materials related to capturing greenhouse ...
... Andrew Lee Lovering, Ph.D., School of Biosciences, University ... Investigator Award for his seminal work on the ... synthesize and modify cell walls in bacteria. Natalie ... of Lovering,s work: "his spectacular abilities in structural ...
Cached Biology News:University of Minnesota receives $13.1 million in DOE funding for 2 new nationwide centers 2University of Minnesota receives $13.1 million in DOE funding for 2 new nationwide centers 3The American Society for Microbiology honors Andrew Lovering 2
... dispensers save space and organize gloves, ... garb. They are the perfect benchtop ... in labs, processing and manufacturing. Clear ... the particles they attract. Many other ...
... spectrofluorometer with a minimum 1 nm resolution ... or greater for the Raman band of ... keep in mind in order to meet ... or anisotropic measurements. ,The FP-6500 is designed ...
... for genome-wide microRNA expression profiling and ... Paraflo microfluidic on-chip synthesis platform. These ... our comprehensive microRNA Expression Profiling Service. ... Maize microRNA Microarray contains all known ...
... 6850 Networked GC is an excellent choice for ... a standard dual-channel GC, this one-channel workhorse can ... the space of one. You get all the ... analyses in an instrument that has the same ...
Biology Products: