Navigation Links
RNA Interference and Gene Silencing: History and Overview

with these characteristics are effective. The reasons for this are unclear but may be a result of positional effects (46, 48, 49). For current recommendations on designing siRNAs, see "siRNA Design".


RNAi as a Tool for Functional Genomics

Although the history and mechanism of RNAi and PTGS are fascinating, many researchers are most excited about RNAi's potential use as a functional genomics tool. Already RNAi has been used to ascertain the function of many genes in Drosophila, C. elegans, and several species of plants. With the knowledge that RNAi can be induced in mammalian cells by the transfection of siRNAs, many more researchers are beginning to use RNAi as a tool in human, mouse and other mammalian cell culture systems.

In early experiments with mammalian cells, the siRNAs were synthesized chemically (Ambion is one of several companies that offer custom siRNA synthesis). Recently, Ambion introduced a kit (the Silencer siRNA Construction Kit) to produce siRNAs by in vitro transcription, which is a less expensive alternative to chemical synthesis, particularly when multiple different siRNAs need to be synthesized. Once made, the siRNAs are introduced into cells via transient transfection. Due to differences in efficacy, most researchers will synthesize 34 siRNAs to a target gene and perform pilot experiments to determine the most effective one. Transient silencing of more than 90% has been observed wi
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Related biology technology :

1. More siRNA Vectors for RNA Interference
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: RNA Interference and Gene Silencing History and Overview

(Date:5/23/2013)... , May 23, 2013 Pressure BioSciences, ... that the Company will host a teleconference to discuss ... a business update. The teleconference information is provided below: ... a.m. Eastern Daylight Time (EDT) Dial-in: (800) ... (302) 607-2001 (Int,l) Passcode: VS22823 For those ...
(Date:5/23/2013)... 23, 2013 SynCardia Systems, Inc. ( ... FDA, Health Canada and CE (Europe) approved Total Artificial ... Scotland, which boasts more than 380,000 visitors annually, is ... powered by the Freedom® portable driver as part of ... advanced exhibitions of its kind. , “BodyWorks combines ...
(Date:5/23/2013)... (PRWEB) May 23, 2013 Watlow®, ... of complete thermal systems, recently introduced its new ... years of experience in designing and manufacturing industrial ... of products to serve diverse customer needs. , ... featured at the beginning of the book. These ...
(Date:5/22/2013)... MA (PRWEB) May 22, 2013 ... in Alexandria, VA for the Oak Ridge ... which will take place at the Hilton Alexandria ... Biodetection Technologies 2013 conference on June ... Already Participating: , AdSave, American Elements, Applied Research ...
Breaking Biology Technology:Pressure BioSciences, Inc. to Discuss First Quarter 2013 Financial Results and Provide Business Update 2SynCardia Total Artificial Heart Featured in $3 Million Exhibition at Glasgow Science Centre 2SynCardia Total Artificial Heart Featured in $3 Million Exhibition at Glasgow Science Centre 3Watlow Introduces Its New Industrial Heater Catalog 2Biodetection Technologies 2013 and Oak Ridge National Lab’s Biosurveillance Symposium to Bring Together Leading Organizations in Alexandria, VA from June 17-19, 2013 2Biodetection Technologies 2013 and Oak Ridge National Lab’s Biosurveillance Symposium to Bring Together Leading Organizations in Alexandria, VA from June 17-19, 2013 3Biodetection Technologies 2013 and Oak Ridge National Lab’s Biosurveillance Symposium to Bring Together Leading Organizations in Alexandria, VA from June 17-19, 2013 4Biodetection Technologies 2013 and Oak Ridge National Lab’s Biosurveillance Symposium to Bring Together Leading Organizations in Alexandria, VA from June 17-19, 2013 5
... and individuals are invited to enter the ... recognizes benefits coming from research and engineering of ... New Products Awards are given in four major ... companies (500-999 employees); medium companies (50-499 employees); and ...
... Area Technical College's Network Security Specialist degree program, ... to computer systems and data, has earned federal certification by ... . , ,It is only one of two in the ... had to match its curriculum precisely to what the NSA ...
... could be launched from Wisconsin in the next ten years, ... Committee 14-1 and could reach the Senate for a vote ... of a private spaceport in Wisconsin that would be regulated ... a Wisconsin Aerospace Authority to regulate private space travel in ...
Cached Biology Technology:Space port could open in Sheboygan in next 10 years 2Space port could open in Sheboygan in next 10 years 3
(Date:5/23/2013)... carrying more than 284 million tons of cargo, transit the ... toll fees for the Panama Canal Authority. Each time a ... are used from Gatun Lake, which is also a source ... isthmus. , However, the advent of very large "super" ... at sea, has demanded change. The Panama Canal is being ...
(Date:5/23/2013)... wrapping of genomic DNA around nucleosomes in the ... A team of Ludwig-Maximilians-Universitaet (LMU) in Munich now ... be locally displaced from nucleosomes for transcription. , ... in the cell nucleus, wrapped around disk-shaped particles ... four different histone proteins and accommodating two loops ...
(Date:5/23/2013)... A new report from the Institute of Medicine ... in at least 60 minutes of vigorous or moderate ... than half of American youth meet current evidence-based guidelines ... intensity physical activity daily, according to the report, which ... school for nearly half of their waking hours, the ...
Breaking Biology News(10 mins):Reforestation study shows trade-offs between water, carbon and timber 2Reforestation study shows trade-offs between water, carbon and timber 3Biochemistry: Unspooling DNA from nucleosomal disks 2Schools should provide students with daily physical activity, IOM recommends 2
... suggest that past climate changes and sea level fluctuations may ... region of South America. Today, the Amazon basin ... yet the reasons why this came to be are not ... three species of leafcutter ants from Central and South America ...
... of the most pressing international issuessuch as renewable energy ... pressing than ever that an informed public be capable ... The Ecological Society of America (ESA),s 93rd Annual Meeting, ... importance of using a broad range of tools to ...
... of Texas at Austin is part of a team ... the water desalination process, increasing access to fresh water ... the desalination process more efficient with better membranes, it ... water, which will expand the availability of clean water ...
Cached Biology News:Study suggests past climate changes may have promoted the formation of new species in the Amazon 2Training future scientists at the Ecological Society of America's 93rd Annual Meeting 2New chlorine-tolerant, desalination membrane hopes to boost access to clean water 2
... Vibratome Company has joined forces with the ... Vibratome 800 Tissue Chopper. This tissue sectioning ... of tissue for metabolic experiments and to ... specimens available at biopsy or from small ...
... Custom Peptide Services offer a full ... your research needs. Services include: Peptide ... Peptide synthesis, purified product (70-95% ... (acetylation, amidation, biotinylation, fluorescein, myristylation, phosphorylation, and ...
... an integrated confocal laser scanner designed to ... your specific application. GeneFocus can recommend a ... system to your specifications. The Open ... been modularized and built onto an optical ...
...
Biology Products: