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RNA Amplification Just Got Better: MessageAmp II

o transcription and aRNA purification. Reagents for 20 reactions and a detailed Instruction Manual are included.



Figure 3. Replicate Analysis of Two Amplification Protocols. Normalized array results are plotted from replicate amplifications. Both MessageAmp II (top) and Affymetrix standard protocol (bottom) were used to amplify with the identical total RNA samples (1 g), and equivalent amounts of cRNA were hybridized on matched lot Human Genome Focus arrays. All IVT reactions were performed for 4 hours. Correlation values are included and indicate a measure of reproducibility with both methods.


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Cat# Product Name Size 1751 MessageAmp II aRNA Amplification Kit 20 rxns
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