Navigation Links
RNA Amplification Just Got Better: MessageAmp II

ield in MessageAmp II. A comparison of ArrayScript RT (AS; 200 U), SuperScript II (SSII; 200 U); wild type M-MLV RT (200 U), and wild type AMV RT (10 U) in MessageAmp II conditions demonstrates that ArrayScript outperforms other commercial RTs. 90 ng HeLa-S3 total RNA (28S/18S ratio = 1.7) was heat denatured in the presence of T7 Oligo(dT) at 70C for 10 minutes. Reverse transcription was initiated with the RT enzyme and incubated at 42C for 2 hr in an air incubator. Following first strand cDNA synthesis, DNA polymerase was added for second strand synthesis, which was incubated at 16C for 2 hr in a thermocycler (heated lid option turned off). The double-stranded cDNA was then filter purified and added to a 4 hr in vitro transcription reaction. Last, the resulting aRNA was purified by glass filter and eluted in nuclease-free water for analysis by Agilent 2100 bioanalyzer and NanoDrop Spectrophotometer.


An Improved Amplification Kit
The RT and second-strand cDNA synthesis improvements, combined with the use of MEGAscript technology in the in vitro transcription reaction (IVT) result in the ability to use shortened IVT incubation times, and the generation of expression information from total RNA samples <1 g. In two round amplification reactions, the MessageAmp II Kit facilitates robust amplification of total RNA from samples as low as 100 pg.

The MessageAmp II aRNA Amplification Kit contains all the necessary reagents for first-strand cDNA synthesis, RNase H digestion, second-strand synthesis, cDNA purification, in vitr
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification
2. Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels
3. Greater Amplification Specificity with New Hot Start PCR Enzyme
4. Enhanced Amplification of Long Targets with PfuTurbo DNA Polymerase
5. Optimizing pfuturbo DNA Polymerase Amplification Reactions with Perfect Match PCR Enhancer
6. Improve Amplification Specificity with Hot Start PCR Enzyme
7. Amplification of the amoA Gene of Nitrosococcus oceani Bacteria Using Eppendorf MasterTaq
8. Amplification of Mouse cDNAs for Microarrays Using the Eppendorf MasterTaq Kit
9. Optimizing the Direct Amplification of Missing cDNA 5 Ends Using the Eppendorf Mastercycler gradient
10. Optimizing DNA Amplification Protocols using the Eppendorf Mastercycler
11. Amplification of Epstein-Barr Virus Exon C with Difficult Primers Using Eppendorf Mastercycler gradient
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: RNA Amplification Just Got Better MessageAmp

(Date:7/24/2014)... PARK, Calif. , July 24, 2014 /PRNewswire/ ... company in the emerging field of regenerative medicine, ... webcast of a presentation to investors on Tuesday, ... p.m. PDT.  The presentation will include an overview ... To access the webcast, visit http://www.ustream.tv/channel/asterias-biotherapeutics ...
(Date:7/24/2014)... computers have yet to materialise. Yet, scientists are ... such computers faster. One such approach relies on ... by applying an electric field. A new study ... coupled quantum dots (TQDs) with electrical impulses can ... example, should TQDs be used as quantum information ...
(Date:7/24/2014)... 24, 2014 Research and Markets ... and Proteomics Analytical Instruments Market 2014-2018" report to ... Genomics is the study of the gene ... the structure and functions of proteomes or sets of ... technology. Genomics involves the mapping of genes and DNA ...
(Date:7/24/2014)... July 24, 2014 Three companies from ... in Livestrong’s Big C Competition. Out of 700 competition ... headed to the semi-final round. In this round twenty ... accelerator program, complete with mentoring from thought-leaders and medical ... part of the angelMD commitment to the Livestrong vision ...
Breaking Biology Technology:Asterias Biotherapeutics Announces Live Investor Webcast 2Asterias Biotherapeutics Announces Live Investor Webcast 3Asterias Biotherapeutics Announces Live Investor Webcast 4Global Genomics and Proteomics Analytical Instruments Market 2014-2018: Key Vendors are Agilent, Bio-Rad , F. Hoffmann, La Roche, Illumina, PerkinElmer and Thermo Fisher Scientific 23 angelMD Startups Make Livestrong Big C Semi-finals 2
... SAN DIEGO, July 24 Orexigen Therapeutics, Inc. ... fully exercised their overallotment option to purchase an additional 1,500,000 ... announced public offering that priced on July 23, 2009. ... at a public offering price of $7.50 per share, resulting ...
... , , , ... NES-ZIONA, Israel, July 24 PROLOR Biotech, Inc. (OTC Bulletin Board: PBTH), formerly ... private investors to sell up to $10 million of the company,s newly issued ... As part of the agreements, the company closed on the issuance of one ...
... , ... it used to be. ,Affordable biometric technology means you can,t get someone to buddy punch for you, ... from anywhere on the web, at anytime! , ... NSW (PRWEB) July 24, 2009 -- Technology is putting an end to the cat and mouse game ...
Cached Biology Technology:Orexigen(R) Therapeutics Announces Full Exercise of Overallotment Option by Underwriters 2PROLOR Biotech Announces $10 Million Preferred Stock Line From Members of The Frost Group 2PROLOR Biotech Announces $10 Million Preferred Stock Line From Members of The Frost Group 3PROLOR Biotech Announces $10 Million Preferred Stock Line From Members of The Frost Group 4Time Clocks Technology Catches Up With Crooks 2Time Clocks Technology Catches Up With Crooks 3
(Date:7/24/2014)... average over a 35 year period in which the ... of humans on declining animal numbers. This decline matters ... spiders, crustaceans, slugs and worms bring to our day-to-day ... for nutrient cycling, water filtration and human health. , ... by UCL, Stanford and UCSB, focused on the demise ...
(Date:7/24/2014)... two factors that characterize sustainable university and college ... qualified physics teachers. Specifically, one or more faculty ... in combination with institutional motivation and commitment can ... Engineering and Math (STEM) teacher shortages are especially ... way for institutions seeking to increase the number ...
(Date:7/24/2014)... 3.5 billion years of evolutionary trial and error, is ... may be reaching a tipping point. , In a ... published in Science , an international team of ... is contributing to what appears to be the early ... , Since 1500, more than 320 terrestrial vertebrates have ...
Breaking Biology News(10 mins):Invertebrate numbers nearly halve as human population doubles 2Invertebrate numbers nearly halve as human population doubles 3Creating sustainable STEM teacher preparation programs 2Stanford biologist warns of early stages of Earth's 6th mass extinction event 2Stanford biologist warns of early stages of Earth's 6th mass extinction event 3
... March 24, 2008) New BARACLUDE (entecavir) data ... resistance in nucleoside-nave patients through five years of ... patients analyzed, no additional patient developed resistance in ... treatment, the cumulative probability of developing mutations in ...
... HIV-positive African adults who become infected with Salmonella die ... diarrhea. Now, UC Davis School of Medicine scientists have ... findings also implicate a mechanism by which HIV evades ... have found the defect in the immune response that ...
... most often produced from biomass burning, cooking with solid ... the atmosphere three to four times greater than prevailing ... in the journal Nature Geoscience. Scripps Institution of ... and University of Iowa chemical engineer Greg Carmichael, said ...
Cached Biology News:BARACLUDE data show low resistance over 5 years in nucleoside-naive hepatitis B patients 2BARACLUDE data show low resistance over 5 years in nucleoside-naive hepatitis B patients 3UC Davis researchers discover how HIV turns food-poisoning into lethal infection 2UC Davis researchers discover how HIV turns food-poisoning into lethal infection 3Black carbon pollution emerges as major player in global warming 2Black carbon pollution emerges as major player in global warming 3
Mouse monoclonal antibody raised against a partial recombinant ZNF167. NCBI Entrez Gene ID = ZNF167...
...
...
... The Amplifluor UniPrimer-fluorescein is a hairpin ... transfer pair (fluorescein and non-fluorescent quencher) ... The UniPrimer is identical to ... Universal Detection System (S7901) and is ...
Biology Products: