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RNA Amplification Just Got Better: MessageAmp II

Kit (patent pending) incorporates extensive improvements to the original MessageAmp aRNA Amplification Kit. Improving amplification involves more than simply increasing the final yield of labeled cRNA. Consistency with other amplification methods is also important so data can be compared across methods. Microarray expression profiling data from MessageAmp II and the Affymetrix recommended protocol for GeneChip analysis (see sidebar, Obtain Reproducible Microarray Data) show a high level of concordance. The new MessageAmp II Kit provides a wide dynamic range of RNA input (100 pg-5 g), and a single round of amplification on samples as small as 100 ng generates sufficient cRNA for GeneChip analysis.


Improved Yields of Full-length cDNA
Even before developing MessageAmp II, it was apparent that the efficiencies of the reverse transcription step and the second strand cDNA synthesis reaction were critical for generating high yields of labeled cRNA with minimal change to the representation of message abundance in total RNA samples. This observation led to further optimization of the second strand cDNA synthesis reaction and the development of ArrayScript, an M-MLV reverse transcriptase specifically engineered to generate high yields of full-length cDNA.

Higher Quality cDNA. Ambion's new ArrayScript produces higher
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