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RNA Amplification Just Got Better: MessageAmp II


Compatible with GeneChip analysis

Amplify as little as 100 ng RNA in a single round

Includes the new ArrayScript engineered M-MLV RT, engineered to maximize yields of full- length cDNA

cRNA synthesis powered by MEGAscript, the best in vitro transcription technology

Consistently delivers high percent Present Calls and low 3'/5' ratios in GeneChip analysis

Gene arrays simultaneously measure the expression levels of thousands of genes in a single experiment and have become the most widely used method for assessing gene expression on a genome wide scale. The primary limitation of this technology is the requirement for large amounts of RNA for array hybridization. This is especially a problem when working with limited samples, such as small biopsies, mRNA-deficient cells and tissues, primary cell cultures, and laser capture microdissection (LCM) samples. This hurdle is usually overcome by using T7 RNA polymerase driven transcription of cDNA obtained from total RNA samples to generate sufficient amounts of amplified antisense or copy RNA (aRNA or cRNA). This amplification method is commonly referred to as the "Eberwine method" [1], "IVT method" or the "aRNA or cRNA method".

Ambion's new MessageAmp II aRNA Amplification
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