Navigation Links
RNA, DNA, and Protein from a Single Sample

Reagent is suitable for use in real-time and end-point RT-PCR, amplification/labeling for microarray analyses, in vitro translation, cDNA synthesis, Northern blotting, and RNase protection assays. RNA preparations isolated using bromochloropropane often do not require an additional DNase treatment, but Ambion's TURBO DNA-free Kit can be used to remove residual genomic DNA before RT-PCR assays. In addition, PCR, Southern blotting, and cloning can be performed using DNA, and denaturing polyacrylamide gel electrophoresis, and Western blot assays can be performed using protein isolated with TRI Reagent. See www.ambion.com/prod/tri for more information and detailed protocols.

Figure 1. Bromochloropropane (Phase Separation Reagent) Improves Genomic DNA Removal in the TRI Reagent Single-Step RNA Isolation Method. Total RNA was isolated from RNAlater-treated mouse liver, kidney, brain, lung, heart, and spleen and from fresh cultured human cells using the standard TRI reagent protocol with either bromochloropropane (BCP) or chloroform (CHCl3).(A) Genomic DNA removal was assessed by one-step, (+) or () RT, quantitative PCR targeting mouse or human TATA binding protein (TBP) mRNA and gene sequences, and is presented as percent of residual gDNA. (B) Total RNA yield was determined by A260 using the NanoDrop spectrophotometer, and RNA yield (g/mg) was normalized to mg of mouse tissue input or to 0.5 x 105 human cultured cells.


Scientific Contributors

Quoc Hoang, WeiWei Xu, Ro
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. A New C-Terminal GST Vector for Protein Production in S. pombe
2. New Mammalian Two-Hybrid System Detects Protein-Protein Interactions
3. Antibodies for Studying NMDA Receptor Protein Expression and Synapse-Specific Immunolabeling
4. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
5. New Yeast Cloning System for Producing Proteins with Native Amino Acid Sequences
6. Optimized Imaging of Protein Gels Stained with Coomassie Brilliant Blue Dye
7. High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli
8. Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
9. Yeast Protein Production System Features High Yields and One-Step Purification
10. Efficient Cleavage of Fusion Proteins to Yield Native Amino Termini
11. Expression and Purification of Recombinant Proteins That Have Native Amino Acid Sequence
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: RNA DNA and Protein from Single Sample

(Date:6/18/2013)... 18, 2013 DuPont welcomes 48 of ... annual DuPont National AgriScience Teachers Ambassador Academy this week ... Chestertown, Md. , The NATAA “Ag Academy” ... is a special project of the National FFA ... Educators (NAAE) . The highly recommended agriscience teachers, ...
(Date:6/18/2013)... 18, 2013 The Pistoia ... the HELM biomolecular representation standard software toolkit and ... HELM (Hierarchical Editing Language for Macromolecules) ... biomolecules (e.g. proteins, nucleotides, antibody drug conjugates) whose ... informatics methodologies impractical or unusable. HELM solves this ...
(Date:6/18/2013)... 18, 2013  AMRI (NASDAQ: AMRI ... Mass. , site has received approval to ... registration to handle Schedule 2 and 2N controlled ... Burlington,s physical security and ... (Logo: http://photos.prnewswire.com/prnh/20120229/NY61160LOGO ) ...
(Date:6/18/2013)... MINNEAPOLIS , June 18, 2013  Techne ... today that it has agreed to acquire 100% ... subsidiary Bionostics, Inc. (Bionostics).  Bionostics is a global ... control solutions that verify the proper operation of ... utilized in point of care blood glucose and ...
Breaking Biology Technology:DuPont Sponsors National AgriScience Ambassador Academy 2DuPont Sponsors National AgriScience Ambassador Academy 3The Pistoia Alliance Releases HELM Biomolecular Representation Standard Open Source Tools 2AMRI Burlington Receives DEA Approval to Handle Controlled Substances 2Techne Corporation Announces Acquisition Agreement 2Techne Corporation Announces Acquisition Agreement 3Techne Corporation Announces Acquisition Agreement 4Techne Corporation Announces Acquisition Agreement 5
... Liko, a leading supplier of,patient lifting equipment, has ... multi-purpose handling straps designed to assist,caregivers with a ... the Multi-Strap product line consists of a highly ... repetitive,daily physical tasks incurred by healthcare caregivers including ...
... form Hebei Kiwa ... Huaxing Bio-Pharmaceuticals Co., Ltd., BEIJING and CLAREMONT, ... (OTC Bulletin Board: KWBT),announced entry into a contract for ... The joint venture company aims at,developing and producing Kiwa,s ...
... 27 Informed Medical Communications,(IMC), a leading provider ... announced the appointment of Bert,Marchio to the position ... http://www.newscom.com/cgi-bin/prnh/20080512/NYM105LOGO ), Marchio brings to IMC ... positions in both the public and private sectors, ...
Cached Biology Technology:New Liko Multiple Purpose Lifting Straps Facilitate Patient Care Tasks 2Kiwa Bio-Tech Joint Venture to Produce Preventative for Avian Flu 2Kiwa Bio-Tech Joint Venture to Produce Preventative for Avian Flu 3Informed Medical Communications Appoints New Chief Financial Officer 2
(Date:6/18/2013)... refers to a chemical modification of DNA and ... in the DNA sequence. Until now, scientists believed ... of certain genes. Today, a team of researchers ... by Emmanouil Dermitzakis, Louis-Jeantet Professor at the Faculty ... the case and that DNA methylation may play ...
(Date:6/17/2013)... a new virus in patients with severe brain infections ... the virus is responsible for the symptoms of disease. ... 28 out of 644 patients with severe brain infections ... in any of the 122 patients with non-infectious brain ... and central nervous system are often fatal and patients ...
(Date:6/17/2013)... the concept of improving original foundations with more modern ... University of Pittsburgh,s Kenneth P. Dietrich School of Arts ... could make drug delivery, gas storage, and gas transport ... were reported in the latest issue of the ... ). , The recent work builds upon Pitt Associate ...
Breaking Biology News(10 mins):The secret of DNA methylation 2New virus isolated from patients with severe brain infections 2New virus isolated from patients with severe brain infections 3'Chemical architects' build materials with potential applications in drug delivery and gas storage 2
... today at the International Liver Congress 2012 demonstrates that ... bile acid metabolism.(1) Bile acids are synthesised from ... gut microbiota into secondary bile acids. The main function ... fat. In addition, hepatic synthesis of bile acids is ...
... most crucial act that plants undergo, as the fruits of ... seeds from which the next generation grows. While ... the timing of their flowering in response to environmental conditions, ... little has been known about what exactly triggers plants to ...
... 27, the New York Academy of Sciences will ... Symposium, focusing on the design of oligosaccharides and ... the symposium will focus on the following areas: ... and the role of these macromolecules in materials ...
Cached Biology News:Scientists discover 'switch' in plants to create flowers 2