Navigation Links
RNA, DNA, and Protein from a Single Sample

Now Available: TRI Reagent
Use one reagent to isolate DNA-free RNA, RNA-free DNA, and/or protein

Obtain higher yields than traditional guanidine thiocyanate or cesium chloride methods

Compatible with many sample sources (e.g., human, plant, yeast, bacterial, or viral samples)

Easily scalable depending on starting material and amountof nucleic acid or protein needed

Fast, simple complete sample processing in ~1 hour


TRI Reagent (patented product/reagent) is a ready-to-use reagent for the isolation of total RNA or the simultaneous isolation of RNA, DNA, and protein from diverse biological samples. During a one-step sample homogenization/ lysis procedure, TRI Reagent disrupts cells and denatures endogenous nucleases. The homogenate is separated into aqueous and organic phases by addition of bromochloropropane (or chloroform) followed by centrifugation (see sidebar, Advantages of Using Bromochloropropane Instead of Chloroform). RNA partitions to the aqueous phase, DNA to the interphase, and protein to the organic phase.

This highly reliable technique performs well with both small and large quantities of tissues and cultured cells (e.g., samples larger than ~5 mg tissue or 5 x 105 cultured cells) (Figure 1). RNA isolated using TRI Reagent is suitable for use in real-time and end-point RT-PCR, amplification/labeling for microarray analyses, in vitro translation, cDNA synthesis, Northern blotting, and RNase protection assays. RNA preparations isolated using bromochloropropane often do not require an additional DNase treatment, but Ambion's TURBO DNA-free Kit can be used to remove residual genomic DNA before RT-PCR assays. In addition, PCR, Southern blotting, and cloning can be performed using DNA, and denaturing polyacrylamide gel electrophoresis, and Western blot assays can be performed using protein isolated with TRI Reagent. See www.ambion.com/prod/tri for more information and detailed protocols.

Figure 1. Bromochloropropane (Phase Separation Reagent) Improves Genomic DNA Removal in the TRI Reagent Single-Step RNA Isolation Method. Total RNA was isolated from RNAlater-treated mouse liver, kidney, brain, lung, heart, and spleen and from fresh cultured human cells using the standard TRI reagent protocol with either bromochloropropane (BCP) or chloroform (CHCl3).(A) Genomic DNA removal was assessed by one-step, (+) or () RT, quantitative PCR targeting mouse or human TATA binding protein (TBP) mRNA and gene sequences, and is presented as percent of residual gDNA. (B) Total RNA yield was determined by A260 using the NanoDrop spectrophotometer, and RNA yield (g/mg) was normalized to mg of mouse tissue input or to 0.5 x 105 human cultured cells.


Scientific Contributors

Quoc Hoang, WeiWei Xu, Ro y Chris Willis, Angela Burrell, Mangkey Bounpheng, Xingwang Fang Ambion, Inc.


back to top


Ordering Information
Cat# Product Name Size 1907 TURBO DNA-free 50 rxns 9738 TRI Reagent 100 ml
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. A New C-Terminal GST Vector for Protein Production in S. pombe
2. New Mammalian Two-Hybrid System Detects Protein-Protein Interactions
3. Antibodies for Studying NMDA Receptor Protein Expression and Synapse-Specific Immunolabeling
4. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
5. New Yeast Cloning System for Producing Proteins with Native Amino Acid Sequences
6. Optimized Imaging of Protein Gels Stained with Coomassie Brilliant Blue Dye
7. High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli
8. Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
9. Yeast Protein Production System Features High Yields and One-Step Purification
10. Efficient Cleavage of Fusion Proteins to Yield Native Amino Termini
11. Expression and Purification of Recombinant Proteins That Have Native Amino Acid Sequence
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:2/12/2016)... DIEGO , Feb. 12, 2016 Biocom, the ... science community, took a group of San Diego ... as part of its 2016 Precision Medicine Advocacy Fly-In. Biocom ... the Food and Drug Administration (FDA), the Centers for Medicare ... as well as San Diego U.S. Representatives Susan Davis ...
(Date:2/12/2016)... 12, 2016  PTC Therapeutics, Inc. (NASDAQ: ... (Strategies to Realize Innovation, Vision and Empowerment) grant ... provides funds to patient advocacy organizations to develop ... contributions to the rare disease community by increasing ... patient advocates. Mary Frances Harmon ...
(Date:2/11/2016)... DIEGO, Feb. 11, 2016  Neurocrine Biosciences, Inc. (NASDAQ: NBIX ... ended December 31, 2015. --> ... a net loss of $29.3 million, or $0.34 loss per share, ... per share for the same period in 2014. For the year ... $88.9 million, or $1.05 loss per share, as compared to a ...
(Date:2/11/2016)... NEW YORK , Feb. 11, 2016  Bioethics International, ... how medicines are researched, developed, marketed and made accessible to ... BMJ Open had named the publication of the ... for 2015. The publication is also featured as one of ... published in the last year that are most frequently read. ...
Breaking Biology Technology:
(Date:2/8/2016)... 8, 2016 Worldcore ... presents innovation for clients, comfort and unbeatable security, ... --> Worldcore is the first ... clients, comfort and unbeatable security, with a Voice ... Worldcore is the first EU-regulated global ...
(Date:2/3/2016)... --> --> Fourth quarter 2015:   ... compared with fourth quarter of 2014. Gross margin was 46% ... Earnings per share increased to SEK 6.39 (loss: 0.49). ... , --> --> January ... (233.6), up 1,142% compared with 2014. Gross margin was 43% ...
(Date:2/3/2016)... DUBLIN , Feb. 3, 2016 /PRNewswire/ ... announced the addition of the "Emotion ... NLP, Machine Learning, and Others), Software Tools ... Application Areas, End Users,and Regions - Global ... offering. --> http://www.researchandmarkets.com/research/d8zjcd/emotion_detection ) ...
Breaking Biology News(10 mins):