Navigation Links
RNA, DNA, and Protein from a Single Sample

Now Available: TRI Reagent
Use one reagent to isolate DNA-free RNA, RNA-free DNA, and/or protein

Obtain higher yields than traditional guanidine thiocyanate or cesium chloride methods

Compatible with many sample sources (e.g., human, plant, yeast, bacterial, or viral samples)

Easily scalable depending on starting material and amountof nucleic acid or protein needed

Fast, simple complete sample processing in ~1 hour


TRI Reagent (patented product/reagent) is a ready-to-use reagent for the isolation of total RNA or the simultaneous isolation of RNA, DNA, and protein from diverse biological samples. During a one-step sample homogenization/ lysis procedure, TRI Reagent disrupts cells and denatures endogenous nucleases. The homogenate is separated into aqueous and organic phases by addition of bromochloropropane (or chloroform) followed by centrifugation (see sidebar, Advantages of Using Bromochloropropane Instead of Chloroform). RNA partitions to the aqueous phase, DNA to the interphase, and protein to the organic phase.

This highly reliable technique performs well with both small and large quantities of tissues and cultured cells (e.g., samples larger than ~5 mg tissue or 5 x 105 cultured cells) (Figure 1). RNA isolated using TRI
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. A New C-Terminal GST Vector for Protein Production in S. pombe
2. New Mammalian Two-Hybrid System Detects Protein-Protein Interactions
3. Antibodies for Studying NMDA Receptor Protein Expression and Synapse-Specific Immunolabeling
4. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
5. New Yeast Cloning System for Producing Proteins with Native Amino Acid Sequences
6. Optimized Imaging of Protein Gels Stained with Coomassie Brilliant Blue Dye
7. High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli
8. Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
9. Yeast Protein Production System Features High Yields and One-Step Purification
10. Efficient Cleavage of Fusion Proteins to Yield Native Amino Termini
11. Expression and Purification of Recombinant Proteins That Have Native Amino Acid Sequence
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: RNA DNA and Protein from Single Sample

(Date:5/20/2013)... May 20, 2013 The World Molecular ... appointment of Lisa Baird as Executive Director. Baird ... 2013. The WMIS vision is to encompass ... understand and effectively treat diseases in the developed and ... was established in 2011 by integrating the Academy of ...
(Date:5/17/2013)... Topricin Pain Relief and Healing Cream, ... among nationally recommended over-the-counter (OTC) health products 2012 ... News" Health in collaboration with "Pharmacy Times." ... Pharmacists Recommended designation” in the “Topical Analgesics” category, ... is the only homeopathic formula to earn that ...
(Date:5/17/2013)... rejection occurs when the transplant recipient,s immune system identifies ... It was previously thought that T cells, the immune ... molecules known as chemokines in order to migrate to ... Journal of Clinical Investigation , Fadi Lakkis and colleagues ... that chemokine stimulation of T cells is not required ...
(Date:5/17/2013)... UK (PRWEB) May 17, 2013 The ... Photonics21 at the association’s recent annual meeting ... including EC Vice-President Neelie Kroes, who called the action ... sectors, and a stimulant to European innovation.” , The ... structure of the new Photonics PPP at its 29 ...
Breaking Biology Technology:WMIS Appoints Lisa Baird as Next Executive Director 2Pharmacists' Picks: Topical BioMedics' Topricin Listed on Top Recommended OTC Health Products 2SPIE Joins in Praise for ‘Huge Step Forward’ for Innovation R&D Enabled by Photonics21 Actions 2SPIE Joins in Praise for ‘Huge Step Forward’ for Innovation R&D Enabled by Photonics21 Actions 3
... Alfacell Corporation (OTC Bulletin Board: ACEL) today reported ... U.S. Food and Drug Administration (FDA) to discuss the ... ONCONASE rolling New Drug Application (NDA) for the treatment ... pre-NDA meeting, the FDA provided guidance to the company ...
... PUBLISHED BY UNIVERSAL , NETTLETON, Miss., Jan. 27 ... that certain unauthorized inaccurate information is being published ... website, potentially other websites and publications, and an ... company has found errors in statements concerning the ...
... Scheduled for Tuesday, February 3, 2009 SAN DIEGO, Jan. ... ) announced today that the Company will report its ... Nasdaq market closes on Tuesday, February 3, 2009. Neurocrine ... to discuss its financial results and provide a Company ...
Cached Biology Technology:Alfacell Provides ONCONASE(R) NDA Submission Update 2Alfacell Provides ONCONASE(R) NDA Submission Update 3Neurocrine Biosciences Announces Conference Call and Webcast to Present Fourth Quarter and Year End 2008 Financial Results 2
(Date:5/17/2013)... a group of proteins in the brain responsible for ... target that could increase cell survival. , The discovery, ... and published in the EMBO journal with ... new therapies for stroke and other brain diseases. , ... which identified a protein, known as SUMO, responsible for ...
(Date:5/17/2013)... An international team of scientists ... and cell movement inside a living frog embryo in ... new method to advance biological research and the search ... University and the Karlsruher Institut fr Technologie in Germany, ... U.S. Department of Energy,s Argonne National Laboratory, released the ...
(Date:5/16/2013)... ancient shorelines to predict the stability of today,s largest ice ... from three million years ago, for example when Earth ... be evidence of a high sea level due to ice ... scientists to think that if the world,s largest ice sheets ... same in our modern, progressively warming world. , However, ...
Breaking Biology News(10 mins):SUMO wrestling cells reveal new protective mechanism target for stroke 2New X-ray method shows how frog embryos could help thwart disease 2World's biggest ice sheets likely more stable than previously believed 2
... Technological University (NTU), and Israel,s number one university, ... new collaboration to jointly offer a Doctor of ... PhD degree programme is a university-wide agreement and ... Bioengineering. Significantly, the joint PhD degree will also ...
... the study and manipulation of human embryonic stem cells ... competition for a Hebrew University of Jerusalem researcher. ... Benvenisty of the Silberman Institute of Life Sciences was ... the Hebrew University of Jerusalem Board of Governors 73rd ...
... Guam,s territorial entomologist and Aubrey Moore, UOG extension entomologist, ... early June. Jackson was invited to assist in ... (Oryctes rhinoceros) population. This virus only infects rhino ... of the pest on other Pacific islands. The ...
Cached Biology News:NTU ramps up collaboration with Israel's top university 2NTU ramps up collaboration with Israel's top university 3Research on stem cells wins first prize for Hebrew University researcher 2Research on stem cells wins first prize for Hebrew University researcher 3Unusual rhino beetle behavior discovered 2