Navigation Links
RNA, DNA, and Protein from a Single Sample

Now Available: TRI Reagent
Use one reagent to isolate DNA-free RNA, RNA-free DNA, and/or protein

Obtain higher yields than traditional guanidine thiocyanate or cesium chloride methods

Compatible with many sample sources (e.g., human, plant, yeast, bacterial, or viral samples)

Easily scalable depending on starting material and amountof nucleic acid or protein needed

Fast, simple complete sample processing in ~1 hour


TRI Reagent (patented product/reagent) is a ready-to-use reagent for the isolation of total RNA or the simultaneous isolation of RNA, DNA, and protein from diverse biological samples. During a one-step sample homogenization/ lysis procedure, TRI Reagent disrupts cells and denatures endogenous nucleases. The homogenate is separated into aqueous and organic phases by addition of bromochloropropane (or chloroform) followed by centrifugation (see sidebar, Advantages of Using Bromochloropropane Instead of Chloroform). RNA partitions to the aqueous phase, DNA to the interphase, and protein to the organic phase.

This highly reliable technique performs well with both small and large quantities of tissues and cultured cells (e.g., samples larger than ~5 mg tissue or 5 x 105 cultured cells) (Figure 1). RNA isolated using TRI Reagent is suitable for use in real-time and end-point RT-PCR, amplification/labeling for microarray analyses, in vitro translation, cDNA synthesis, Northern blotting, and RNase protection assays. RNA preparations isolated using bromochloropropane often do not require an additional DNase treatment, but Ambion's TURBO DNA-free Kit can be used to remove residual genomic DNA before RT-PCR assays. In addition, PCR, Southern blotting, and cloning can be performed using DNA, and denaturing polyacrylamide gel electrophoresis, and Western blot assays can be performed using protein isolated with TRI Reagent. See www.ambion.com/prod/tri for more information and detailed protocols.

Figure 1. Bromochloropropane (Phase Separation Reagent) Improves Genomic DNA Removal in the TRI Reagent Single-Step RNA Isolation Method. Total RNA was isolated from RNAlater-treated mouse liver, kidney, brain, lung, heart, and spleen and from fresh cultured human cells using the standard TRI reagent protocol with either bromochloropropane (BCP) or chloroform (CHCl3).(A) Genomic DNA removal was assessed by one-step, (+) or () RT, quantitative PCR targeting mouse or human TATA binding protein (TBP) mRNA and gene sequences, and is presented as percent of residual gDNA. (B) Total RNA yield was determined by A260 using the NanoDrop spectrophotometer, and RNA yield (g/mg) was normalized to mg of mouse tissue input or to 0.5 x 105 human cultured cells.


Scientific Contributors

Quoc Hoang, WeiWei Xu, Ro y Chris Willis, Angela Burrell, Mangkey Bounpheng, Xingwang Fang Ambion, Inc.


back to top


Ordering Information
Cat# Product Name Size 1907 TURBO DNA-free 50 rxns 9738 TRI Reagent 100 ml
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. A New C-Terminal GST Vector for Protein Production in S. pombe
2. New Mammalian Two-Hybrid System Detects Protein-Protein Interactions
3. Antibodies for Studying NMDA Receptor Protein Expression and Synapse-Specific Immunolabeling
4. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
5. New Yeast Cloning System for Producing Proteins with Native Amino Acid Sequences
6. Optimized Imaging of Protein Gels Stained with Coomassie Brilliant Blue Dye
7. High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli
8. Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
9. Yeast Protein Production System Features High Yields and One-Step Purification
10. Efficient Cleavage of Fusion Proteins to Yield Native Amino Termini
11. Expression and Purification of Recombinant Proteins That Have Native Amino Acid Sequence
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:3/23/2017)... Research and Markets has announced the addition of the ... ... The Global Market for Bioproducts Should Reach $714.6 Billion by ... 8.9%, This research report quantifies the two ... segments: bio-derived chemicals, biofuels, pharmaceuticals (biodrugs and herbal/botanicals), biocomposite materials, ...
(Date:3/23/2017)... 2017  BioPharmX Corporation (NYSE MKT: BPMX), a ... market, today reported financial results for the quarter ... provide an update on the company,s clinical development ... "We are pleased to report that last year ... President Anja Krammer. "We achieved key clinical milestones ...
(Date:3/23/2017)... 23, 2017 According to a report by ... market is fragmented due to the presence of a large pool of ... Thermo Fisher , and Sigma-Aldrich, compete with each other in this ... held more than 76% of this market in 2016.  ... As of now, a large ...
(Date:3/22/2017)... N.Y. , March 22, 2017 Regeneron Pharmaceuticals, ... the Regeneron Genetics Center (RGC), U.K. Biobank and GSK to ... the U.K. Biobank resource. The initiative will enable researchers to ... new medicines for a wide range of serious and life ... ...
Breaking Biology Technology:
(Date:2/22/2017)... LONDON , Feb. 22, 2017 ... by 2021, ABI Research identifies four technologies that ... solutions to secure significant share in the changing ... payments, and passive authentication.   "Companies ... it comes to security," says Dimitrios Pavlakis ...
(Date:2/14/2017)... WINSTON-SALEM, N.C. , Feb. 14, 2017  Wake ... FRY-shlog), M.D., as its new chief executive officer (CEO). ... succeeds CEO John D. McConnell , M.D., who ... new position at the Medical Center, after leading it ... oversee the full scope of Wake Forest Baptist,s academic ...
(Date:2/9/2017)... , Feb. 9, 2017 The biomass ... analysis of the biomass boiler market globally in terms ... of biomass boilers. The market for biomass boilers has ... type, end-user, application, and country/region. The market based on ... & forest residues, biogas & energy crops, urban residues, ...
Breaking Biology News(10 mins):