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RNA, DNA, and Protein from a Single Sample

Now Available: TRI Reagent
Use one reagent to isolate DNA-free RNA, RNA-free DNA, and/or protein

Obtain higher yields than traditional guanidine thiocyanate or cesium chloride methods

Compatible with many sample sources (e.g., human, plant, yeast, bacterial, or viral samples)

Easily scalable depending on starting material and amountof nucleic acid or protein needed

Fast, simple complete sample processing in ~1 hour


TRI Reagent (patented product/reagent) is a ready-to-use reagent for the isolation of total RNA or the simultaneous isolation of RNA, DNA, and protein from diverse biological samples. During a one-step sample homogenization/ lysis procedure, TRI Reagent disrupts cells and denatures endogenous nucleases. The homogenate is separated into aqueous and organic phases by addition of bromochloropropane (or chloroform) followed by centrifugation (see sidebar, Advantages of Using Bromochloropropane Instead of Chloroform). RNA partitions to the aqueous phase, DNA to the interphase, and protein to the organic phase.

This highly reliable technique performs well with both small and large quantities of tissues and cultured cells (e.g., samples larger than ~5 mg tissue or 5 x 105 cultured cells) (Figure 1). RNA isolated using TRI
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