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RNA & cDNA Purification Using RNAClean

ng precipitation or column-based reagents. LiCl preferentially precipitates RNA (Barlow et al.,1963), but requires a series of manual steps including chilling, washing and multiple centrifugations. LiCl precipitation has also been linked to decreased performance in downstream in vitro translation reactions possibly due to inhibition by chloride ions (Maniatis et al., 1989). Column purification does not require chilling, but it does require multiple centrifugation steps which make automation more challenging.

Solid Phase Reversible Immobilization (SPRI) is a patented magnetic bead-based purification technology (Hawkins et al., 1995). Nucleic acids are immobilized onto magnetic beads allowing for easy contaminant removal and sample washing. Purified nucleic acids are easily eluted in aqueous solutions. Optimization of SPRI immobilization buffers allows for selective isolation of specific nucleic acid species. Importantly, SPRI requires no centrifugation or filtration steps which enables simplified automation.


RNAClean Purification
RNAClean purification of RNA or cDNA is faster, easier and more efficient than traditional methods such as LiCl precipitation. We compared purification of RNA ladders containing a wide range of fragment sizes (0.16 - 9.5kb) using LiCl or RNAClean. RNAClean had a higher percent recovery than standard LiCl precipitation for the entire range of RNA sizes (Figure 1, Table 1). Importantly, the smaller RNA fragments purified using RNAClean maintained the same proportional representation as in the unpurified samples.


Recovery at Low Concentrations
RNAClean is a versatile reagent that provides efficient
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