Navigation Links
RNA & cDNA Purification Using RNAClean

ng precipitation or column-based reagents. LiCl preferentially precipitates RNA (Barlow et al.,1963), but requires a series of manual steps including chilling, washing and multiple centrifugations. LiCl precipitation has also been linked to decreased performance in downstream in vitro translation reactions possibly due to inhibition by chloride ions (Maniatis et al., 1989). Column purification does not require chilling, but it does require multiple centrifugation steps which make automation more challenging.

Solid Phase Reversible Immobilization (SPRI) is a patented magnetic bead-based purification technology (Hawkins et al., 1995). Nucleic acids are immobilized onto magnetic beads allowing for easy contaminant removal and sample washing. Purified nucleic acids are easily eluted in aqueous solutions. Optimization of SPRI immobilization buffers allows for selective isolation of specific nucleic acid species. Importantly, SPRI requires no centrifugation or filtration steps which enables simplified automation.


RNAClean Purification
RNAClean purification of RNA or cDNA is faster, easier and more efficient than traditional methods such as LiCl precipitation. We compared purification of RNA ladders containing a wide range of fragment sizes (0.16 - 9.5kb) using LiCl or RNAClean. RNAClean had a higher percent recovery than standard LiCl precipitation for the entire range of RNA sizes (Figure 1, Table 1). Importantly, the smaller RNA fragments purified using RNAClean maintained the same proportional representation as in the unpurified samples.


Recovery at Low Concentrations
RNAClean is a versatile reagent that provides efficient
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli
2. Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels
3. Yeast Protein Production System Features High Yields and One-Step Purification
4. Expression and Purification of Recombinant Proteins That Have Native Amino Acid Sequence
5. Easy Cosmid DNA Purification with StrataPrep Kits
6. MinElute PCR Purification Kit
7. QIAquick PCR Purification Kit
8. Microarray Target Purification Kit and the Microarray Target Synthesis Kit (T7)
9. Double-Affinity Purification of GST-His Tagged Proteins Using Vivapure 8-to-96 Well Cobalt-Chelate Kit
10. Ultra-Fast Purification of Small Nucleic Acids
11. RNAClean - RNA & cDNA in vitro Reaction Purification
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: RNA cDNA Purification Using RNAClean

(Date:1/22/2015)... CAMBRIDGE, Mass. , Jan. 22, 2015   GenoSpace , ... suite of tools to enable the broad use of genomic, ... today announced the appointment of Michelle Munson , CEO ... independent director. Logo - http://photos.prnewswire.com/prnh/20150122/170713 ...
(Date:12/25/2014)... The report “Service Quality Management and Telco ... Type - Global Advancements, Worldwide Forecasts & Analysis (2014-2019)” ... and forecasting of revenues. , Browse 152 market ... in-depth TOC on “Service Quality Management (SQM) and Telco ...
(Date:12/24/2014)... and NEW YORK , Dec. ... biopharmaceutical company advancing patient care in critical areas, announced the ... common stock, and warrants to purchase up to an aggregate ... $4.00 per share and $.01 per warrant.  The warrants have ...
(Date:12/24/2014)... BOTHELL, Wash. , Dec. 24, 2014   BioLife Solutions ... manufacturer and marketer of proprietary clinical grade hypothermic storage ... thermal shipping products for cells and tissues  ("BioLife" or ... Annual Meeting of Stockholders on May 4, 2015 (the "Annual ...
Breaking Biology Technology:GenoSpace Expands Board with Appointment of Michelle Munson 2Service Quality Management Market worth $2.1 Billion & Telco Customer Experience Management Market worth $2.3 Billion by 2019 – New Report by MarketsandMarkets 2Service Quality Management Market worth $2.1 Billion & Telco Customer Experience Management Market worth $2.3 Billion by 2019 – New Report by MarketsandMarkets 3Service Quality Management Market worth $2.1 Billion & Telco Customer Experience Management Market worth $2.3 Billion by 2019 – New Report by MarketsandMarkets 4PlasmaTech Biopharmaceuticals, Inc. Announces Closing of Public Offering 2PlasmaTech Biopharmaceuticals, Inc. Announces Closing of Public Offering 3BioLife Solutions Sets Date for Annual Meeting of Stockholders 2
... Keck Graduate Institute,s (KGI) Biotechnology Entrepreneurship program today announced ... Dec. 8, 2008. , , ... individual investors who fund early-stage, technology and high-growth companies in ... in becoming angel investors. The educational session begins at ...
... disease,diagnostics market has demonstrated sustained growth due to ... occurring in the,market, promoting technology and product integration. ... policies for autoimmune diagnostic tests,persists, posing a challenge ... (Logo: http://www.newscom.com/cgi-bin/prnh/20081117/FSLOGO ) , ...
... JOSE, Calif., Nov. 21 VNUS(R) Medical,Technologies, Inc. (Nasdaq: ... minimally invasive treatment of venous reflux disease, today announced its,plans ... to be,held December 2-3, 2008, at The New York Palace ... Brian Farley, President and Chief Executive,Officer. , ...
Cached Biology Technology:KGI Entrepreneur Program Hosts Angel Investing Educational Meeting 2Advances in Detection Technology Drive the European Autoimmune Disease Diagnostics Market 2Advances in Detection Technology Drive the European Autoimmune Disease Diagnostics Market 3
(Date:1/22/2015)...  A man-made form of insulin delivered by nasal spray ... adults with mild cognitive impairment and Alzheimer,s disease dementia, ... Wake Forest Baptist Medical Center. The study,s ... impairment (MCI) or mild to moderate Alzheimer,s dementia (AD). ...
(Date:1/22/2015)... Research and Markets ( http://www.researchandmarkets.com/research/wxdglm/global_wearable ) ... Wearable Technologies Market and Applications, Opportunities, Industry Analysis, ... 2014-2020" report to their offering. , ... can be worn on the user,s body and ...
(Date:12/22/2014)... 22, 2014 Research and Markets ( ... "The Global Watermarking and Fingerprinting Markets" ... http://photos.prnewswire.com/prnh/20130307/600769 This insight provides ... and fingerprinting markets. Watermarking aims to control and ...
Breaking Biology News(10 mins):Nasal Spray with Insulin Equivalent Shows Promise as Treatment for Adults with Mild Cognitive Impairment, Alzheimer's Dementia 2Nasal Spray with Insulin Equivalent Shows Promise as Treatment for Adults with Mild Cognitive Impairment, Alzheimer's Dementia 3Global Wearable Technologies Market and Applications, Opportunities, Industry Analysis, Segmentation, Size, Growth, Share, Overview, Trends and Forecast 2014-2020 2The Global Watermarking and Fingerprinting Markets 2
... found for the first time in a variety of freshwater ... , According to experts at the Aquatic Animal Health ... septicemia virus (VHSV), which causes fatal anemia and hemorrhaging in ... poses no threat to humans. , In May ...
... at the University of California, San Diego and three other ... inter-related responses that cells use to avoid becoming diseased or ... The group led by UCSD bioengineering professor Trey Ideker describe ... of gene control that is triggered by chemical damage to ...
... Iranian scientists have linked a chemical that plays an ... damage and male infertility, the 22nd annual conference of ... , Dr Iraj Amiri, embryology laboratory director at the ... years nitric oxide (NO) has been recognised as a ...
Cached Biology News:Cornell researchers find serious fish virus in Northeast for first time 2Heal thyself: Systems biology model reveals how cells avoid becoming cancerous 2Heal thyself: Systems biology model reveals how cells avoid becoming cancerous 3Study links high levels of nitric oxide to infertility and sperm DNA damage 2
... Kit ADAM17 Activity Kit TNF-α Converting Enzyme ... FROM LIGHT. AVOID FREEZE/THAW CYCLES. The InnoZyme™ ... sensitive assay designed to measure human TACE ... and for screening enzyme inhibitors. An Anti-Human ...
... GELase™ Agarose Gel-Digesting Preparation contains a unique ... simple, quantitative recovery of intact DNA and ... gels following electrophoresis in TAE, TBE, MOPS, ... digested directly in the TAE, TBE, MOPS, ...
... Premixed solution for high PCR specificity ... the combination of enzyme with unique ... nonspecific amplification provided by the ... handling convenient Master Mix and ...
Recombinant Cat Interferon Alpha Activity: 2.7 x 10 7 u/mg...
Biology Products: