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RNA & cDNA Purification Using RNAClean


RNAClean is a SPRI-based nucleic acid purification system that efficiently isolates RNA or cDNA from in vitro transcription, cDNA or RNA labeling and antisense RNA amplification reactions. As gene expression profiling analysis and the use of microarrays and RNAi technologies grow, a highly effective, automated purification reagent is required. Traditional purification reagents can be time consuming, less efficient over a wide range of fragment sizes and not easily automated. This application note demonstrates the high quality of nucleic acids purified using RNAClean versus precipitation and column technologies.


Introduction
As the genomics revolution moves from gene discovery to gene function analysis, techniques requiring RNA manipulation are becoming more prominent. Development of antisense RNA (aRNA) amplification technology has revolutionized gene expression analysis by allowing experimentation from minute amounts of starting RNA materials. Another technical breakthrough is RNAi, which is rapidly becoming an important tool for manipulating gene expression. The use of RNAi treatment coupled with detailed microarray studies based on aRNA amplification is a powerful combination of techniques useful for understanding gene expression and functional data. Procuring this type of information will be aided greatly by a high throughput method for purifying RNA or cDNA from the in vitro reactions associated with microarray and RNAi studies, namely cDNA synthesis, in vitro transcription, aRNA amplification and cDNA/RNA labeling.


Purification Technologies
Purification of RNA and cDNA from in vitro reactions has traditionally been performed usi
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