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REPLI-g UltraFast Mini Kit

the sample (10 ng purified genomic DNA, 0.5 l whole blood, or 300 tissue culture cells) undergoes gentle alkaline denaturation, avoiding fragmentation and damage of template DNA. Next the sample is neutralized, and finally incubated with REPLI-g UltraFast DNA Polymerase at 30C. The amplified DNA is ready to use after 60 minutes without further purification. The average DNA yield is 10 g per 20 l reaction after 90 minutes. If time is very limited, sufficient product is available for downstream genetic analysis after just 45 minutes (Figure 1).



Reliable control reactions
Negative control samples (without template DNA) are not amplified, providing a reliable indicator of the presence/absence of sample or contaminating DNA (Figure 1).


Applications
REPLI-g amplified genomic can be used in a variety of downstream applications, including:
SNP and STR genotyping
RFLP and Southern blot analysis
Comparative genome hybridization (CGH)
PCR and real-time PCR
Microarray analysis


Reference
1. Hosono, S. et al. (2003) Unbiased whole-genome amplification directly from clinical samples. Genome Res. 13, 954.





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