For the fastest reliable whole genome amplification
The REPLI-g UltraFast Mini Kit provides highly uniform whole genome amplification from a variety of sample types in just 6090 minutes. The new REPLI-g UltraFast DNA Polymerase allows fast, accurate amplification of genomes, even from limited samples, and generates DNA suitable for direct use in a range of genetic analyses.
Benefits of the REPLI-g UltraFast Mini Kit include:
Time savings fast and easy sample amplification in just 6090 minutes
Reliable amplification no change in locus or sequence representation and no amplification in the absence of template
Versatility optimized protocols for different sample types
High-fidelity whole genome amplification (WGA)
REPLI-g UltraFast technology provides fast and highly uniform DNA amplification across the entire genome, with negligible sequence bias (1). The method is based on Multiple Displacement Amplification (MDA) technology isothermal genome amplification utilizing a new, uniquely processive DNA polymerase that gives DNA products of up to 100 kb in length in a much shorter time while still avoiding the high sequence bias of PCR-based amplification methods. In addition, a unique alkaline denaturation buffer guarantees gentle denaturation of genomic DNA and avoids fragmentation of template DNA caused by heat denaturation methods. This ensures more sensitive and more reliable results in downstream assays.
Fast amplification of small samples
Amplification of genomic DNA using the REPLI-g UltraFast Mini Kit involves 3 basic steps (see flowchart). First,