Navigation Links
REPLI-g UltraFast Mini Kit

For the fastest reliable whole genome amplification


The REPLI-g UltraFast Mini Kit provides highly uniform whole genome amplification from a variety of sample types in just 6090 minutes. The new REPLI-g UltraFast DNA Polymerase allows fast, accurate amplification of genomes, even from limited samples, and generates DNA suitable for direct use in a range of genetic analyses.


Benefits of the REPLI-g UltraFast Mini Kit include:

Time savings fast and easy sample amplification in just 6090 minutes

Reliable amplification no change in locus or sequence representation and no amplification in the absence of template

Versatility optimized protocols for different sample types





High-fidelity whole genome amplification (WGA)

REPLI-g UltraFast technology provides fast and highly uniform DNA amplification across the entire genome, with negligible sequence bias (1). The method is based on Multiple Displacement Amplification (MDA) technology isothermal genome amplification utilizing a new, uniquely processive DNA polymerase that gives DNA products of up to 100 kb in length in a much shorter time while still avoiding the high sequence bias of PCR-based amplification methods. In addition, a unique alkaline denaturation buffer guarantees gentle denaturation of genomic DNA and avoids fragmentation of template DNA caused by heat denaturation methods. This ensures more sensitive and more reliable results in downstream assays.


Fast amplification of small samples
Amplification of genomic DNA using the REPLI-g UltraFast Mini Kit involves 3 basic steps (see flowchart). First, the sample (10 ng purified genomic DNA, 0.5 l whole blood, or 300 tissue culture cells) undergoes gentle alkaline denaturation, avoiding fragmentation and damage of template DNA. Next the sample is neutralized, and finally incubated with REPLI-g UltraFast DNA Polymerase at 30C. The amplified DNA is ready to use after 60 minutes without further purification. The average DNA yield is 10 g per 20 l reaction after 90 minutes. If time is very limited, sufficient product is available for downstream genetic analysis after just 45 minutes (Figure 1).



Reliable control reactions
Negative control samples (without template DNA) are not amplified, providing a reliable indicator of the presence/absence of sample or contaminating DNA (Figure 1).


Applications
REPLI-g amplified genomic can be used in a variety of downstream applications, including:
SNP and STR genotyping
RFLP and Southern blot analysis
Comparative genome hybridization (CGH)
PCR and real-time PCR
Microarray analysis


Reference
1. Hosono, S. et al. (2003) Unbiased whole-genome amplification directly from clinical samples. Genome Res. 13, 954.





Visit www.qiagen.com/goto/REPLI-g to discover the benefits of REPLI-g technology today!


back to top
'"/>

Source:


Page: All 1 2

Related biology technology :

1. REPLI-g Mitochondrial DNA Kit
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:2/8/2016)... 8, 2016  Diplomat Pharmacy, Inc. (NYSE: DPLO) announced today that its new website has gone ... story-driven, knowledge-based and mobile-friendly. Visit the new site: ... ... ... "The goal was to reimagine the ...
(Date:2/5/2016)... 5, 2016  In the pharmaceutical industry the medical ... of launch activities including the identification and engagement of ... is especially high in the oncology therapeutic area where ... and the Role of Medical Affairs in Oncology Launch ... oncology therapies find better ways to utilize medical affairs ...
(Date:2/4/2016)... New FDA action date of July 22, ... July 22, 2016   --> - ... - Lifitegrast has the potential to be the only ... signs and symptoms of dry eye disease in adults --> ... approved in the U.S. in the past decade indicated for the treatment of ...
(Date:2/4/2016)... , Feb. 4, 2016  CytoSorbents Corporation (NASDAQ: ... commercializing its flagship CytoSorb® blood filter to treat ... around the world, announced that CEO Dr. ... the Source Capital Group,s 2016 Disruptive Growth & ... the company.  Conference Presentation Details: ...
Breaking Biology Technology:
(Date:1/11/2016)... 11, 2016  higi, the leading retail and ... locations, web and mobile, today announced it has ... existing investors. --> ... further innovate higi,s health platform – its network ... – including expanding services and programs to retail ...
(Date:1/7/2016)... 7, 2016 This BCC Research report studies ... technologies and devices, identifying newer markets and exploring the ... of biometric devices. Includes forecast from 2015 to 2020. ... markets and explore the expansion of the present application ... type of biometric technology, determine its current market size, ...
(Date:1/6/2016)... PLEASANTON, Calif. , Jan. 6, 2016  Varam ... SayPay as their trusted partner to deliver advanced authentication ... other financial services to the poor. A loan of ... in their lives, giving them the ability to purchase ... machine to make clothing, or stock for a local ...
Breaking Biology News(10 mins):