For reliable whole genome amplification from human mitochondrial DNA in total DNA samples
The REPLI-g Mitochondrial DNA Kit provides specific and uniform whole genome amplification from human mitochondrial DNA in total DNA samples. The simple procedure enriches for mitochondrial DNA with minimal contamination from nuclear DNA, thus avoiding the need for time-consuming isolation of mitochondrial DNA and increasing the sensitivity of downstream assays.
Benefits of the REPLI-g Mitochondrial DNA Kit include:
High sensitivity of downstream assays due to enrichment of mitochondrial DNA
Accurate and reproducible results even from small total DNA samples
Versatility suitable for human DNA isolated from various starting materials, including hair
Fast and easy protocol with all reagents included in the kit
Working with mitochondrial DNA
One limitation to the use of mitochondrial DNA has been the need to isolate it from nuclear DNA, particularly in cases where the sensitivity of mitochondrial DNA marker assays needs to be increased. The isolation procedure involves many time-consuming steps and leads to substantial losses of mitochondrial DNA. The REPLI-g Mitochondrial DNA Kit overcomes this limitation by enriching for mitochondrial DNA sequences in total DNA samples.
High fidelity mitochondrial specific amplification
REPLI-g Mitochondrial DNA technology provides fast and highly uniform DNA amplification across the entire mitochondrial genome. The method is based on Multiple Displacement Amplification (MDA) technology, which carries out isothermal genome amplification utilizing a uniquely process