Navigation Links
QuickLyse Miniprep Kit

For ultra-fast, sequencing grade plasmid DNA minipreps


The QIAGEN QuickLyse Miniprep Kit uses one-step lysis technology for ultra-fast preparation of up to 15 g plasmid DNA. The quality of the DNA ensures robust performance in applications such as sequencing, restriction analyses, and cloning.


Benefits of the QuickLyse Miniprep Kit include:

One-step lysis reduced number of handling steps saves time

Reproducible yields sufficient plasmid DNA for any downstream application (Figure 1)

Pure plasmid DNA suitable for standard molecular biology applications



One-step lysis for fast results
Unlike most protocols that use a 3-step lysis procedure, the QuickLyse Miniprep Kit combines enzymatic and osmotic processes to lyse bacterial cells in a single, 3-minute step. In addition, QuickLyse technology uses fewer buffers, simplifying handling, and saving time: 24 plasmid DNA minipreps can be prepared in less than 22 minutes.


Optimized procedure for simplified handling
Bacterial cells are resuspended and lysed and DNA is bound in a single buffer, enabling fast DNA purification with minimal hands-on time. After a 3-minute lysis step, the clear lysate is applied to a QuickLyse Spin Column without any additional centrifugation step. Plasmid DNA binds to the column membrane and is eluted after a single wash step. The isolated DNA can be used directly in standard applications, including automated sequencing, PCR, restriction analysis, and cloning.


High yields of high-quality DNA
The QuickLyse Miniprep Kit yields s ufficient plasmid DNA for any downstream application, including automated sequencing (Figure 2). Up to 15 g of high-copy plasmid DNA can be obtained from 1.5 ml of bacterial culture (Figure 3).

Isolated plasmid DNA has OD260/280 ratios of 1.71.9, indicating that the quality and purity is suitable for all sensitive applications. Sequencing read lengths of over 700 bases are easily achieved.









Visit www.qiagen.com to discover the benefits of ultra-fast minipreps today!


back to top
'"/>

Source:


Page: All 1 2

Related biology technology :

1. Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels
2. DirectPrep 96 Miniprep System for cost-effective, high-throughput plasmid DNA purification
3. QIAprep Spin Miniprep Kit
4. Plasmid Miniprep Kit Selection
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:9/14/2017)... ... September 14, 2017 , ... ... launch of its CliniControl™ (CC) product portfolio, clinically-relevant starting and ancillary materials ... of human Mesenchymal Stem/Stromal Cell (hMSC)-based therapies. The CliniControl product portfolio will ...
(Date:9/14/2017)... ... September 14, 2017 , ... Cambridge Semantics , the ... that its Anzo Smart Data Lake has been named a KMWorld Trend-Setting ... that help organizations succeed in surpassing their knowledge management goals. KMWorld searches for ...
(Date:9/12/2017)... NC (PRWEB) , ... September ... ... Inc. ( http://www.globalspecimensolutions.com ) has announced the release of their latest product ... workflows and storage requirements. LabCODE™ offers pre-built capabilities for Biobanking, Pharmacokinetics, ...
(Date:9/12/2017)... PA (PRWEB) , ... September 12, 2017 , ... Facet ... growing life sciences teams, today announced that Tobias Massa, PhD has joined the company’s ... biotech, life sciences, and technology to his Board position. Most recently he served as ...
Breaking Biology Technology:
(Date:5/23/2017)... , May 23, 2017  Hunova, the first robotic gym for ... has been officially launched in Genoa, Italy . The ... and the USA . The technology was ... the market by the IIT spin-off Movendo Technology thanks to a 10 ... Multimedia News Release, please click: ...
(Date:4/24/2017)... , April 24, 2017 ... and partner with  Identity Strategy Partners, LLP (IdSP) ... "With or without President Trump,s March 6, 2017 ... Terrorist Entry , refugee vetting can be instilled with ... resettlement. (Right now, all refugee applications are suspended ...
(Date:4/13/2017)... , April 13, 2017 UBM,s Advanced ... will feature emerging and evolving technology through ... Innovation Summits will run alongside the expo portion of ... sessions, panels and demonstrations focused on trending topics within ... advanced design and manufacturing event will take place June ...
Breaking Biology News(10 mins):