For ultra-fast, sequencing grade plasmid DNA minipreps
The QIAGEN QuickLyse Miniprep Kit uses one-step lysis technology for ultra-fast preparation of up to 15 g plasmid DNA. The quality of the DNA ensures robust performance in applications such as sequencing, restriction analyses, and cloning.
Benefits of the QuickLyse Miniprep Kit include:
One-step lysis reduced number of handling steps saves time
Reproducible yields sufficient plasmid DNA for any downstream application (Figure 1)
Pure plasmid DNA suitable for standard molecular biology applications
One-step lysis for fast results
Unlike most protocols that use a 3-step lysis procedure, the QuickLyse Miniprep Kit combines enzymatic and osmotic processes to lyse bacterial cells in a single, 3-minute step. In addition, QuickLyse technology uses fewer buffers, simplifying handling, and saving time: 24 plasmid DNA minipreps can be prepared in less than 22 minutes.
Optimized procedure for simplified handling
Bacterial cells are resuspended and lysed and DNA is bound in a single buffer, enabling fast DNA purification with minimal hands-on time. After a 3-minute lysis step, the clear lysate is applied to a QuickLyse Spin Column without any additional centrifugation step. Plasmid DNA binds to the column membrane and is eluted after a single wash step. The isolated DNA can be used directly in standard applications, including automated sequencing, PCR, restriction analysis, and cloning.
High yields of high-quality DNA
The QuickLyse Miniprep Kit yields s ufficient plasmid DNA for any downstream application, including automated sequencing (Figure 2). Up to 15 g of high-copy plasmid DNA can be obtained from 1.5 ml of bacterial culture (Figure 3).
Isolated plasmid DNA has OD260/280 ratios of 1.71.9, indicating that the quality and purity is suitable for all sensitive applications. Sequencing read lengths of over 700 bases are easily achieved.
Visit www.qiagen.com to discover the benefits of ultra-fast minipreps today!
back to top