For ultra-fast, sequencing grade plasmid DNA minipreps
The QIAGEN QuickLyse Miniprep Kit uses one-step lysis technology for ultra-fast preparation of up to 15 g plasmid DNA. The quality of the DNA ensures robust performance in applications such as sequencing, restriction analyses, and cloning.
Benefits of the QuickLyse Miniprep Kit include:
One-step lysis reduced number of handling steps saves time
Reproducible yields sufficient plasmid DNA for any downstream application (Figure 1)
Pure plasmid DNA suitable for standard molecular biology applications
One-step lysis for fast results
Unlike most protocols that use a 3-step lysis procedure, the QuickLyse Miniprep Kit combines enzymatic and osmotic processes to lyse bacterial cells in a single, 3-minute step. In addition, QuickLyse technology uses fewer buffers, simplifying handling, and saving time: 24 plasmid DNA minipreps can be prepared in less than 22 minutes.
Optimized procedure for simplified handling
Bacterial cells are resuspended and lysed and DNA is bound in a single buffer, enabling fast DNA purification with minimal hands-on time. After a 3-minute lysis step, the clear lysate is applied to a QuickLyse Spin Column without any additional centrifugation step. Plasmid DNA binds to the column membrane and is eluted after a single wash step. The isolated DNA can be used directly in standard applications, including automated sequencing, PCR, restriction analysis, and cloning.
High yields of high-quality DNA
The QuickLyse Miniprep Kit yields s