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Quantitative end-point RT-PCR gene,,,expression measurement using the,,,Agilent 2100 Bioanalyzer and,,,standardized RT-PCR

> Conclusions
StaRT-PCR in combination with Agilent 2100 bioanalyzer electrophoresis provides results statistically similar to those obtained with agarose gel or ABI 310 electrophoresis, with better reproducibility. StaRT PCR combined with the Agilent 2100 bioanalyzer provides an excellent method for quantitative RT-PCR gene expression measurement for the following reasons:
The analysis can be performed at the end-point of PCR without need for real-time measurement at each cycle of PCR.
It is more sensitive because quantification can be performed at the first cycle that the bands are detectable without need for multiple points to generate a slope.
Data are standardized and may be entered into a common data bank.
High reproducibility.



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